A Graphene-enhanced imaging of microRNA with enzyme-free signal amplification of catalyzed hairpin assembly in living cells

Liu, Haiyun; Tian, Tian; Ji, Dandan; Ren, Na; Ge, Shenguang; Yan, Mei; Yu, Jinghua

doi:10.1016/j.bios.2016.06.015

Cited by 61 publications

(24 citation statements)

References 53 publications

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“…Accurate in detecting relative miRNA in bulk samples, are incapable of detecting cell-to-cell variations, they require pooling miRNA from lysates acquired from groups of cells. 12 - 19 Therefore, a major challenge exists to accurately detect relative miRNA levels in individual live cells. Fluorescence analysis provides new possibilities for measurement and visualization of biomarkers in living cells owing to the simplicity and high sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Two-Color-Based Nanoflares for Multiplexed MicroRNAs Imaging in Live Cells

Li¹,

Huang²,

Yang³

et al. 2018

Nanotheranostics

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MicroRNAs (miRNAs) have become an ideal biomarker candidate for early diagnosis of diseases. But various diseases involve changes in the expression of different miRNAs. Therefore, multiplexed assay of miRNAs in live cells can provide critical information for our better understanding of their roles in cells and further validating of their function in clinical diagnoses. Simultaneous detection of multiple biomarkers could effectively improve the accuracy of early cancer diagnosis. Here, we develop the two-color-based nanoflares for simultaneously detecting two distinct miRNA targets inside live cells. The nanoflares consist of gold nanoparticles (AuNPs) functionalized with a dense shell of recognition sequences hybridized to two short fluorophore-labeled DNA molecules, termed “flares”. In this conformation, the close proximity of the fluorophore to the AuNPs surface leads to quenching of the fluorescence. However, when target miRNAs bind to the recognition sequence, the concomitant displacement of the flare can be detected as a corresponding increase in fluorescence. The results demonstrate that the two-color-based nanoflares can simultaneously detect miR-21 and miR-141 expression levels in various live cancer cells successfully. Compared to the traditional single-color-based nanoflares, the two-color-based nanoflares could offer more reliable and practical information for cancer detection, improving the accuracy of early disease diagnosis.

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Section: Introductionmentioning

confidence: 99%

Two-Color-Based Nanoflares for Multiplexed MicroRNAs Imaging in Live Cells

Li¹,

Huang²,

Yang³

et al. 2018

Nanotheranostics

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“…Prior to binding the complete built‐in TPNcodes to GO x , free GO x was synthesized by a modified Hummers' method. GO x is an oxidized form of graphene that is frequently used as a platform for an induced adoptive fluorescence signal based on fluorescence resonance energy transfer (FRET) in gene detection . It is known that GO x interacts with carbon rings in nucleic acid structures through π–π interactions.…”

Section: Resultsmentioning

confidence: 99%

Topological Transformation‐Based Nanobarcoding for Detection and Enumeration of MicroRNAs and Single Nucleotide Polymorphism

et al. 2019

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RNA biomarkers have been recently reported to be associated tightly with the diagnosis and prognosis of many diseases. Particularly, cancers considered to be a serious threat to primates are known to be vastly dominated by genetic networks where RNA plays a key role. RNAs are thus recognized as a major target group that can be used for numerous cancer treatments and it is still required to identify and enumerate them in an effective manner. Here, a new topological transformation‐based nanobarcoding technique (TNT) is first reported using fluorescence‐DNA barcodes engaged with graphene oxide (GOx) for effectively discriminating short RNAs such as miRNAs and their single nucleotide polyporphisms in tissue and plasma. Through topological transformation into 3D DNA–RNA polygonal structures, various kinds of microRNAs have been read at the same time and analyzed quantitatively. Also, it positively discerned epidermal growth factor receptor (EGFR) mutations known as single base variations of typical lung cancer specific RNAs. A single variant of 0.785% in target EGFR mutations is explicitly detected. It is speculated that the TNT may be a versatile method for polymerase chain reaction (PCR)‐free practical diagnosis of several clinical genetic deviations such as significant biotic RNA and genic fragments and would be a promising alternative to conventional PCR terrains.

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“…Versatile CHA circuits are adopted for practical applications in several ways. The CHA circuits can be performed alone first, and then coupled with DNAzymes, nanoparticles, and biotins to generate signals. In addition, CHA circuits can be used for mediating other isothermal amplification methods to achieve cascade signal amplification…”

Section: Characterization and Development Of Chamentioning

confidence: 99%

Applications of Catalytic Hairpin Assembly Reaction in Biosensing

Liu

Zhang

Xie

et al. 2019

Small

213

129

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Nucleic acids are considered as perfect programmable materials for cascade signal amplification and not merely as genetic information carriers. Among them, catalytic hairpin assembly (CHA), an enzyme‐free, high‐efficiency, and isothermal amplification method, is a typical example. A typical CHA reaction is initiated by single‐stranded analytes, and substrate hairpins are successively opened, resulting in thermodynamically stable duplexes. CHA circuits, which were first proposed in 2008, present dozens of systems today. Through in‐depth research on mechanisms, the CHA circuits have been continuously enriched with diverse reaction systems and improved analytical performance. After a short time, the CHA reaction can realize exponential amplification under isothermal conditions. Under certain conditions, the CHA reaction can even achieve 600 000‐fold signal amplification. Owing to its promising versatility, CHA is able to be applied for analysis of various markers in vitro and in living cells. Also, CHA is integrated with nanomaterials and other molecular biotechnologies to produce diverse readouts. Herein, the varied CHA mechanisms, hairpin designs, and reaction conditions are introduced in detail. Additionally, biosensors based on CHA are presented. Finally, challenges and the outlook of CHA development are considered.

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A Graphene-enhanced imaging of microRNA with enzyme-free signal amplification of catalyzed hairpin assembly in living cells

Cited by 61 publications

References 53 publications

Two-Color-Based Nanoflares for Multiplexed MicroRNAs Imaging in Live Cells

Two-Color-Based Nanoflares for Multiplexed MicroRNAs Imaging in Live Cells

Topological Transformation‐Based Nanobarcoding for Detection and Enumeration of MicroRNAs and Single Nucleotide Polymorphism

Applications of Catalytic Hairpin Assembly Reaction in Biosensing

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