Dual-neodymium magnet-based microfluidic separation device

Abstract

Microfluidic-based separation methods have been highlighted for a number of biological applications, such as single cell analysis, disease diagnostics, and therapeutics. Although a number of previous studies have been carried out to minimize the physical damage and chemical deformations of the sample during the separation process, it still remains a challenge. In this paper, we developed a microfluidic device with dual-neodymium magnet-based negative magnetophoresis for the separation of the microparticles and cells. The poly(ethylene oxide) (PEO) was added to the solution to increase the viscoelasticity of the medium which could assist the sorting of the microparticles in the microfluidic device even at low flow rates, while minimizing damage to the cells and microparticles. Following this method, it was possible to separate 10 and 16 μm microparticles with high efficiency of 99 ± 0.1%, and 97 ± 0.8%, respectively. We also demonstrated the separation of glioblastoma cancer cells and neural stem cells (NSCs) in the microfluidic device.

Cell Separations and Sorting

“…Sample processing was completed in 10 min and was demonstrated with cell lines. Kye et al 89 reported a microfuidic device with dual-neodymium magnet-based negative magnetophoresis for the size-dependent sorting of microparticles and cells using non-Newtonian viscoelastic polyethylene oxide (PEO) solution and ferrofluid. PEO solution increased the viscoelasticity of the medium, which assisted in sorting microparticles in the microfuidic device at low flow rates (7 μL/min) (Figure 4D).…”

Clog-free high-throughput microfluidic cell isolation with multifunctional microposts

“…For active methods external forces are used to achieve high-resolution particle separation, consistently requiring a particle’s unique physical property. Active methods mainly include electrophoresis 11 , magnetophoresis 12 – 14 , negative magnetophoresis 13 , 14 , optical 15 and thermal manipulations 16 . They typically require high-power energy consumption to generate sufficient external forces for separation based on intrinsic properties 11 , 17 .…”

Performance improvement study of an integrated photovoltaic system for offshore power production

“…The first is in May, the month with yearly average solar radiation, and the second is January, the month with the lowest solar radiation and solar hours. 30 The actual solar position and the solar intensity are simulated based on the specified time of year and the specified location on earth using COMSOL Multiphysics. Note that the solar incident and absorption model is valid for both a two-PV system and a two-PV panel system; F I G U R E 1 Schematic diagram of the simulated proposed solar PV system with a close on a through cross-sectional panel view, where the reflection coming from the sea surface is utilized on both front and back surfaces of the two PV panels s (the solar PV panel elevation is at an angle of 50 from the horizontal) [Colour figure can be viewed at wileyonlinelibrary.com] however, the difference in the results depends on the electrochemical model of the module type.…”

“…The selection of the month of January is made to investigate the performance of the considered system during a period of low radiation intensity, while the month of May is considered for the performance in a period of high radiation intensity. The first is in May, the month with yearly average solar radiation, and the second is January, the month with the lowest solar radiation and solar hours 30 . The actual solar position and the solar intensity are simulated based on the specified time of year and the specified location on earth using COMSOL Multiphysics.…”