Clog-free high-throughput microfluidic cell isolation with multifunctional microposts

Venugopal, Dilip; Kasani, Nanda; Manjunath, Yariswamy; Li, Guangfu; Kaifi, Jussuf T.; Kwon, Jae Wan

doi:10.1038/s41598-021-94123-6

Cited by 5 publications

(2 citation statements)

References 38 publications

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“…Other types of devices combine inertial effects with post arrays to separate and capture blood components. For instance, clog-free devices for separating CTCs from blood were designed with different tear-shaped microposts capable of operating at high flow rates (1 mL/min), with reported efficiencies of more than 80% [78]. These micropost shapes exploit rotation-induced lift forces in combination with particle densities that push larger particles further from the micropost wall.…”

Section: Passive Separation Of Blood Cellsmentioning

confidence: 99%

Microfluidic Blood Separation: Key Technologies and Critical Figures of Merit

Torres-Castro,

Acuña-Umaña,

Lesser-Rojas

et al. 2023

Micromachines

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Blood is a complex sample comprised mostly of plasma, red blood cells (RBCs), and other cells whose concentrations correlate to physiological or pathological health conditions. There are also many blood-circulating biomarkers, such as circulating tumor cells (CTCs) and various pathogens, that can be used as measurands to diagnose certain diseases. Microfluidic devices are attractive analytical tools for separating blood components in point-of-care (POC) applications. These platforms have the potential advantage of, among other features, being compact and portable. These features can eventually be exploited in clinics and rapid tests performed in households and low-income scenarios. Microfluidic systems have the added benefit of only needing small volumes of blood drawn from patients (from nanoliters to milliliters) while integrating (within the devices) the steps required before detecting analytes. Hence, these systems will reduce the associated costs of purifying blood components of interest (e.g., specific groups of cells or blood biomarkers) for studying and quantifying collected blood fractions. The microfluidic blood separation field has grown since the 2000s, and important advances have been reported in the last few years. Nonetheless, real POC microfluidic blood separation platforms are still elusive. A widespread consensus on what key figures of merit should be reported to assess the quality and yield of these platforms has not been achieved. Knowing what parameters should be reported for microfluidic blood separations will help achieve that consensus and establish a clear road map to promote further commercialization of these devices and attain real POC applications. This review provides an overview of the separation techniques currently used to separate blood components for higher throughput separations (number of cells or particles per minute). We present a summary of the critical parameters that should be considered when designing such devices and the figures of merit that should be explicitly reported when presenting a device’s separation capabilities. Ultimately, reporting the relevant figures of merit will benefit this growing community and help pave the road toward commercialization of these microfluidic systems.

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Section: Passive Separation Of Blood Cellsmentioning

confidence: 99%

Microfluidic Blood Separation: Key Technologies and Critical Figures of Merit

Torres-Castro,

Acuña-Umaña,

Lesser-Rojas

et al. 2023

Micromachines

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“…29 Similarly, curved microchannels create helical streamlines, resulting in Dean flow, where larger particles experience a lift force that moves them toward the capture zone, while smaller particles pass through a bypass. 30 Another technique involves lateral flow microfluidic sieving, where fluid with mixed particles flows through a filter. Gentle vibrations prevent clogging and ensure continuous operation.…”

Section: Introductionmentioning

confidence: 99%

A method to prevent clogging and clustering in microfluidic systems using microbubble streaming

Bakhtiari,

Kähler

2024

Biomicrofluidics

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This paper presents an innovative strategy to address the issues of clogging and cluster-related challenges in microchannels within microfluidic devices. Leveraging three-dimensional (3D) microbubble streaming as a dynamic solution, our approach involves the controlled activation of microbubbles near channel constrictions, inducing microstreaming with distinctive features. This microstreaming, characterized by a high non-uniform 3D gradient and significant shear stress, effectively inhibits arch formation at constrictions and disintegrates particle clusters, demonstrating real-time prevention of clogging incidents and blockages. This study includes experimental validation of the anti-clogging technique, a detailed examination of microstreaming phenomena, and their effects on clogging and clustering issues. It also incorporates statistical analyses performed in various scenarios to verify the method’s effectiveness and adaptability. Moreover, a versatile control system has been designed that operates in event-triggered, continuous, or periodic modes, which suits different lab-on-a-chip applications and improves the overall functionality of microfluidic systems.

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Laser-assisted fabrication of deterministic lateral displacement structures on P20 die steel masters for microfluidic particle separation

Pandit

Samuel

2022

Appl. Phys. A

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Clog-free high-throughput microfluidic cell isolation with multifunctional microposts

Cited by 5 publications

References 38 publications

Microfluidic Blood Separation: Key Technologies and Critical Figures of Merit

Microfluidic Blood Separation: Key Technologies and Critical Figures of Merit

A method to prevent clogging and clustering in microfluidic systems using microbubble streaming

Laser-assisted fabrication of deterministic lateral displacement structures on P20 die steel masters for microfluidic particle separation

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