Dual modality fluorescence and reflectance hyperspectral imaging: principle and applications

Martin, Matthew; Wabuyele, Musundi B.; Panjehpour, Masoud; Phan, Mary N.; Overholt, Bergein F.; DeNovo, Robert C.; Moyers, Tamberlyn D.; Song, Seong Gon; Vo‐Dinh, Tuan

doi:10.1117/12.604445

Cited by 17 publications

(17 citation statements)

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“…5,6 It is well known that biological tissues exhibit fluorescent properties when excited with ultra-violet light. 2,7,8 It has also been shown that significant differences in these fluorescence properties occur between tumorous and normal tissues. 2,4 These early single-point spectroscopic attempts were highly successful in detecting tumors.…”

Section: Introductionmentioning

confidence: 97%

“…2,7,8 It has also been shown that significant differences in these fluorescence properties occur between tumorous and normal tissues. 2,4 These early single-point spectroscopic attempts were highly successful in detecting tumors. The basic setup utilized a nitrogen laser as an excitation source and was capable of rapid point measurements.…”

Section: Introductionmentioning

confidence: 97%

“…Techniques that monitor quantitative and qualitative differences in naturally occurring cellular fluorophores have been applied to distinguish tumorous from normal tissues. 1,2,3 Various research groups have developed laser-induced fluorescence (LIF) techniques for cancer diagnosis. 4 Our laboratory spearheaded an effort to use LIF as a non-invasive technique for the detection of cancer in esophageal tissues.…”

Section: Introductionmentioning

confidence: 99%

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Hyperspectral fluorescence imaging system for biomedical diagnostics

Martin¹,

Wabuyele²,

Panjehpour³

et al. 2006

Advanced Biomedical and Clinical Diagnostic Systems IV

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An advanced hyper-spectral imaging (HSI) system has been developed for use in medical diagnostics. One such diagnostic, esophageal cancer is diagnosed currently through biopsy and subsequent pathology. The end goal of this research is to develop an optical-based technique to assist or replace biopsy. In this paper, we demonstrate an instrument that has the capability to optically diagnose cancer in laboratory mice. We have developed a real-time HSI system based on state-of-the-art liquid crystal tunable filter (LCTF) technology coupled to an endoscope. This unique HSI technology is being developed to obtain spatially resolved images of the slight differences in luminescent properties of normal versus tumorous tissues. In this report, an in-vivo mouse study is shown. A predictive measure of cancer for the mice studied is developed and shown. It is hoped that the results of this study will lead to advances in the optical diagnosis of esophageal cancer in humans.

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Hyperspectral fluorescence imaging system for biomedical diagnostics

Martin¹,

Wabuyele²,

Panjehpour³

et al. 2006

Advanced Biomedical and Clinical Diagnostic Systems IV

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“…One of the most promising applications for AOTF-based spectral imaging in medicine is endoscopy, which is widely used for in vivo visualization and diagnostics. 4,8,9 Being coupled with rigid or°e xible medical endoscopes, AOTFs provide an extra capability which is re°ectance spectrum quantitative analysis of inspected tissues and organs. It allows, for example, e®ective detection of mouse pancreas pathology and other malignant tissues.…”

Section: Introductionmentioning

confidence: 99%

“…It allows, for example, e®ective detection of mouse pancreas pathology and other malignant tissues. 8 Most of AOTF-based spectral imagers for endoscopic photoluminescence analysis utilize single acousto-optic (AO) cell, which lacks spectral drift and chromatic aberrations of di®racted image. 10 That is why for spectral images matching, an additional correction procedure must be applied that complicates the analysis and makes it time-consuming and less reliable.…”

Section: Introductionmentioning

confidence: 99%

Double-AOTF-based aberration-free spectral imaging endoscopic system for biomedical applications

Мачихин

Пожар

Batshev

2015

J. Innov. Opt. Health Sci.

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The problem of in vivo photoluminescence diagnostics of the tissues accessible by endoscopes is discussed. The spectral imaging module attachable to conventional rigid and°exible medical endoscopes is developed and described. It is based on a double acousto-optical tunable¯lter (AOTF) and a specialized optical coupling system. The module provides wide¯eld of view (FOV), absence of image distortions, random spectral access, fast spectral image acquisition at any wavelength in the visible range and accurate measurement of re°ectance spectrum in each pixel of the image. Images of typical biomedical samples are presented and discussed. Their spectra are compared to the reference data.

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