Hyperspectral fluorescence imaging system for biomedical diagnostics

Abstract

An advanced hyper-spectral imaging (HSI) system has been developed for use in medical diagnostics. One such diagnostic, esophageal cancer is diagnosed currently through biopsy and subsequent pathology. The end goal of this research is to develop an optical-based technique to assist or replace biopsy. In this paper, we demonstrate an instrument that has the capability to optically diagnose cancer in laboratory mice. We have developed a real-time HSI system based on state-of-the-art liquid crystal tunable filter (LCTF) technology coupled to an endoscope. This unique HSI technology is being developed to obtain spatially resolved images of the slight differences in luminescent properties of normal versus tumorous tissues. In this report, an in-vivo mouse study is shown. A predictive measure of cancer for the mice studied is developed and shown. It is hoped that the results of this study will lead to advances in the optical diagnosis of esophageal cancer in humans.

Medical hyperspectral imaging: a review

“…Compared to optical spectroscopy that measures tissue spectra point-by-point, HSI is able to capture images of a large area of tissue and has exhibited great potential in the diagnosis of cancer in the cervix, 19,34,81,154 breast, 45,155 colon, 66,84,117,118,142,[156][157][158][159] gastrointestine, 160,161 skin, 41,97 ovary, 55 urothelial carcinoma, 162 prostate, 52 esophagea, 163 trachea, 164 oral tissue, 20,32,165,166 tongue, 126 lymph nodes, 72 and brain. 98 HSI cancer studies have been performed in the following major aspects: (1) recognizing protein biomarkers and genomic alterations on individual tumor cells in vitro, 167 (2) analyzing the morphological and structural properties of cancer histological specimens to classify the cancer grades, (3) examining the tissue surface to identify precancerous and malignant lesions in vivo, and (4) measuring the tissue blood volume and blood oxygenation to quantify the tumor angiogenesis and tumor metabolism.…”

Investigation of liquid crystal Fabry–Perot tunable filters: design, fabrication, and polarization independence

“…The interest in LCTFs using this variety of concepts was stimulated from their potential use in displays [4,[16][17][18] and telecommunications [6,7,19,20]. The integration of LCTFs into imaging systems started to emerge recently [21][22][23][24][25].…”

Multi-Channel Hyperspectral Fluorescence Detection Excited by Coupled Plasmon-Waveguide Resonance

“…Recently developed hyperspectral fluorescence techniques show great potential for multiplexed research projects [18]. The hyperspectral fluorescence detection technique has obtained increasing popularity for detecting fluorophores in DNA sequencing and microarray applications [19,20].…”