Dual method for the determination of peroxidase activity and total peroxides-iodide leads to a significant increase of peroxidase activity in human sera

Tatzber, Franz; Griebenow, S.; Wonisch, Willibald; Winkler, Rudolf

doi:10.1016/s0003-2697(02)00652-8

Cited by 105 publications

(79 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Serum total peroxide concentrations were determined by a rapid enzymatic in vitro diagnostic assay (TOC ® ) supplied by LDN (Labor Diagnostik Nord, Nordhorn, Germany) as previously described (Tatzber et al 2003). The test system was based on a peroxide/peroxidase reaction using 3,5,3´,5´-tetramethylbenzidine (TMB) as substrate.…”

Section: Serum Total Peroxidesmentioning

confidence: 99%

“…We decided upon fluorescent techniques to specifically measure water-and lipid-soluble antioxidants (Hofer et al 1995;Mayer et al 2001). Peroxidase activity was used to measure the endogenous anti-oxidative capacity (Tatzber et al 2003). Antibodies against oxidized LDL (oLAb) (Tatzber and Esterbauer 1995) and total peroxides (Tatzber et al 2003) were determined as oxidative stress biomarkers.…”

Section: Introductionmentioning

confidence: 99%

“…Peroxidase activity was used to measure the endogenous anti-oxidative capacity (Tatzber et al 2003). Antibodies against oxidized LDL (oLAb) (Tatzber and Esterbauer 1995) and total peroxides (Tatzber et al 2003) were determined as oxidative stress biomarkers. In this prospective pilot study we focused on monitoring of oxidative stress in the course of CABG surgery with special attention to anti-oxidative effects of ascorbic acid and α-tocopherol as well as the anti-inflammatory effects of EPA and DHA (Mori and Beilin 2004).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Antioxidant Supplementation Attenuates Oxidative Stress in Patients Undergoing Coronary Artery Bypass Graft Surgery

Stanger

Aigner

Schimetta³

et al. 2014

Tohoku J. Exp. Med.

Self Cite

View full text Add to dashboard Cite

Ischemia-reperfusion has been reported to be associated with augmented oxidative stress in the course of surgery, which might be causally involved in the onset of atrial fibrillation (AF), the most common arrhythmia after cardiac surgery. We hypothesized that supplementation of antioxidants and n-3 polyunsaturated fatty acids (n-3 PUFAs) might lower the incidence of AF following coronary artery bypass graft (CABG) surgery. In the present study, by monitoring oxidative stress in the course of CABG surgery, we analyzed the efficacy of vitamins (ascorbic acid and α-tocopherol) and/or n-3 PUFAs (eicosapentaenoic acid and docosahexaenoic acid). Subjects (n = 75) were divided into 4 subgroups: control, vitamins, n-3 PUFAs, and a combination of vitamins and n-3 PUFAs. Fluorescent techniques were used to measure the antioxidative capacity, i.e. ability to inhibit oxidation. Total peroxides, endogenous peroxidase activity, and antibodies against oxidized LDL (oLAb) were used as serum oxidative stress biomarkers. Post-operative increase in oxidative stress was associated with the consumption of antioxidants and a simultaneous onset of AF. This was confirmed through an increased peroxide level and a decreased oLAb titer in control and n-3 PUFAs groups, indicating the binding of antibodies to oxidative modified epitopes. In both subgroups that were supplemented with vitamins, total peroxides decreased, and the maintenance of a constant IgG antibody titer was facilitated. However, treatment with vitamins or n-3 PUFAs was inefficient with respect to AF onset and its duration. We conclude that the administration of vitamins attenuates post-operative oxidative stress in the course of CABG surgery.

show abstract

Section: Serum Total Peroxidesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antioxidant Supplementation Attenuates Oxidative Stress in Patients Undergoing Coronary Artery Bypass Graft Surgery

Stanger

Aigner

Schimetta³

et al. 2014

Tohoku J. Exp. Med.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Serum peroxide concentrations were determined by commercially available rapid enzymatic assay (TOC, LND, Germany) as described before (Tatzber et al 2003). The basis of the assay is the reaction of peroxidase with serum peroxides using tetra-methyl-benzidine (TMB, Sigma, USA) as the chromogenic substrate.…”

Section: Total Serum Peroxidesmentioning

confidence: 99%

Ergometry Induces Systemic Oxidative Stress in Healthy Human Subjects

Antončić-Svetina¹,

Šentija

Čipak

et al. 2010

Tohoku J. Exp. Med.

Self Cite

View full text Add to dashboard Cite

“…Serum peroxide concentrations were determined by a rapid enzymatic, in vitro diagnostic assay (TOC -LDN) as previously described (Tatzber et al 2003). The assay is based on a peroxide/peroxidase reaction using tetramethyl-benzidine (TMB) as the chromogenic substrate.…”

Section: Total Serum Peroxidesmentioning

confidence: 99%

Persistent Oxidative Stress after Myocardial Infarction Treated by Percutaneous Coronary Intervention

Nikolić-Heitzler

Rabuzin

Tatzber

et al. 2006

Tohoku J. Exp. Med.

Self Cite

View full text Add to dashboard Cite

[247][248][249][250][251][252][253][254][255] Acute myocardial infarction causing cardiac ischemia is responsible for the majority of cardiac related deaths. Medical interventions that ensure rapid reperfusion, such as percutaneous coronary intervention, are aimed to allow myocardial re-oxygenation. However, this generates reactive oxygen species, resembling ischemiareperfusion type of injury based on oxidative stress. In the present study we monitored dynamic changes of total serum peroxides, total antioxidant capacity and soluble intercellular adhesion molecule-1 as well as the titer of antibodies against oxidized low-density lipoproteins in the blood during the convalescence period of 32 patients with acute myocardial infarction treated by percutaneous coronary intervention. Samples were taken at admittance and at two hours, four hours, three days and seven days following percutaneous coronary intervention. Total antioxidant capacity dropped to 82% ( p < 0.05). The titer of antibodies against oxidized low-density lipoproteins transiently decreased within the first three days, and increased afterwards. The values of serum peroxides and soluble intercellular adhesion molecule-1 increased continuously in respect to the initial levels reaching the maximum at the time of release from hospital. These findings indicate a persistent oxidative stress that might be associated with intravascular inflammation in patients during convalescence and release from hospital. myocardial infarction; percutaneous coronary intervention; inflammation; soluble intercellular adhesion molecule-1; lipid peroxidation © 2006 Tohoku University Medical Press While oxidative stress has been shown to be related with the age and living habits (Ozbay and Dulker 2002), it plays an important role in the development of vascular diseases that affect vital organs, in particular brain and heart (Zarkovic 2003;Zarkovic et al. 2004;Madamanchi et al.

show abstract

Dual method for the determination of peroxidase activity and total peroxides-iodide leads to a significant increase of peroxidase activity in human sera

Cited by 105 publications

References 19 publications

Antioxidant Supplementation Attenuates Oxidative Stress in Patients Undergoing Coronary Artery Bypass Graft Surgery

Antioxidant Supplementation Attenuates Oxidative Stress in Patients Undergoing Coronary Artery Bypass Graft Surgery

Ergometry Induces Systemic Oxidative Stress in Healthy Human Subjects

Persistent Oxidative Stress after Myocardial Infarction Treated by Percutaneous Coronary Intervention

Contact Info

Product

Resources

About