Dual‐layer column filtration cleanup and gas chromatography‐tandem mass spectrometry detection for the analysis of 39 pesticide residues in porcine meat

Abstract

A rapid and effective method was developed for the analysis of 39 pesticide residues in porcine meat with dual-layer multiplug filtration cleanup and gas chromatographytandem mass spectrometry detection. The cleanup process was performed with columns packed with two layers, namely multiwalled carbon nanotubes, C18 and anhydrous magnesium sulfate (MgSO 4 ) as top layer, while mixture of florisil and MgSO 4 as bottom layer. A single-layer method was tested in parallel, with columns packed with the same amount of absorbents. Extraction conditions and filtration cleanup process were optimized to obtain satisfied method performance. Method linearity was calculated with coefficients of determination more than 0.995. The limits of quantitation were verified with acceptable accuracy at the lowest spiked concentration of 0.01 mg/kg (except pyrimethanil). The recoveries at three fortified levels (0.01, 0.05, and 0.1 mg/kg) in five replicates were between 74 and 118% (except pyrimethanil) with relative standard deviations range from 1 to 16%. The matrix effects were in the range of 1.01 to 2.84. This new method was applied for the analysis of multipesticide residues in market samples of porcine meat. This study showed the dual-layer multiplug filtration cleanup demonstrated better performance than that with the single-layer columns in cleanup of porcine meat.

Comprehensive Strategy for Sample Preparation for the Analysis of Food Contaminants and Residues by GC–MS/MS: A Review of Recent Research Trends

“…Certain substrates present in foods, such as tea, honeybees, meat, or leek, introduce a heavy matrix interference, which could cause poor peak shape or suppress ionization of analytes or low content target analytes. In this context, further dilution of extracts or the use of a clean-up process, such as USE, multiplug filtration clean-up (m-PFC), robotic clean-up, deep-frozen, SPE methods, are recommended to effectively reduce matrix interference [84][85][86]. The m-PFC method provides a more practical and effective way to perform the clean-up process than the conventional d-SPE method, offering a compromise between the clean-up performance of SPE and the convenience of d-SPE [44].…”

Determination of Multi-pesticides Residues in Jasmine Flower and Its Scented Tea

“…While pushing and pulling the piston, the extract interacts with the sorbents so that the interfering substances are adsorbed. In previous studies (Qin et al 2016a;Qin et al 2017b;Song et al 2020), this method was successfully applied to determine pesticide residues from complex matrices. Compared with d-SPE procedure, the m-PFC method is shown to be more rapid without solvent evaporation, vortex and centrifugation steps (Qin et al 2015;Zou et al 2016; Pszczolinska and Kociolek 2022).…”

Thyroid Dysfunction Induced by Fungicide Famoxadone Exposure Contributes to Nonalcoholic Fatty Liver Disease in Male Mice: In Vivo, In Vitro, and In Silico Studies

“…Famoxadone is a widely used fungicide for the control of Ascomycete, Basidiomycete, and Oomycete classes of fungi in fruits, vegetables, cereals, and other crops, which is one of the top five sales fungicides of Corteva Agriscience Co., Ltd. in 2018 . Famoxadone was detected in tomatoes, − raisins, watermelons, and pork and the concentration of famoxadone could reach 2045 μg/kg in water and 1916 μg/kg in soil after being applied to the field . In some regions, the concentration of famoxadone even exceeded the maximum residue level in vegetables, leading to high intake risk .…”