Dual-fluorophore silica microspheres for ratiometric acidic pH sensing

The rise of bio-inspired polymer compartments responding to pathology-related signals

“…35 For further improvement of the stability, sensors with a stable silica core and additional silica NPs in the outer shell have been fabricated by the LbL method. 221 The resulting porous microspheres have been proven to be promising candidates for ratiometric acidic pH sensing. In the future, such structures could be optimized by using a diversity of dyes to allow multianalyte detection.…”

Kinetic and spectroscopic responses of pH-sensitive nanoparticles: influence of the silica matrix

“…69 Furthermore, the fairly high intensity change upon pH changes simplies the subsequent analyses and can be, at least partially, traced back to the smaller size as compared to other nanoparticle preparations. 31,[34][35][36]85 In addition, the particles consist of hardly toxic components and are smaller than most well-known nanoprobes. Therefore, our particles are ideally suited to track the intracellular pH in living cells as exemplied in live-cellular microscopy.…”

“…[29][30][31][32] Although labeled silica nanoparticles with self-calibrating pH detection exist, preferential ratiometric detection is established by a mixture of two or more different uorophores. 22,23,30,31,[33][34][35][36][37][38] In this work, two-channel readout is achieved by a single probe molecule, which provides two reversibly interconverting detection signals. The use of a single probe molecule offers the advantage in which the pH measurement is independent of parameters such as local and irreproducible probe composition or preferential photobleaching of one dye.…”

Luminescence-Based Optical Sensors Fabricated by Means of the Layer-by-Layer Nano-Assembly Technique

“…Ideally, both have the same absorption spectrum and a complementary emission spectrum [ 52 ], which allows the use of a single excitation light source and the simultaneous monitoring of the emission peaks: one of them will change in the presence of the target analyte, while the other will remain constant. Apart from encapsulating both luminophores [ 58 ], it is also common that one of them is assembled as part of the multilayered coating [ 78 ].…”