Dual‐fluorophore Raspberry‐like Nanohybrids for Ratiometric pH Sensing

Acquah, Isaac; Roh, Jinkyu; Ahn, Dong June

doi:10.1002/asia.201700616

Cited by 4 publications

(1 citation statement)

References 35 publications

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“…Similar results were obtained in the presence of other hROS species, demonstrating that the DEFN nanoprobes can be used for rapid and sensitive sensing and imaging of hROS in living cells. Similar dual-emission core-satellite nanohybrids have also been applied for ratiometric sensing and imaging of metal ions, 216–223 pH, 224, 225 ROS, 226 small molecules with biological activity, 227, 228 temperature, 229 and tumor hypoxia. 230 Besides, a binary heterogeneous assembled nanohybrid with dual-emission was recently reported by Qu and colleagues for ratiometric sensing of hROS, where the nanohybrid, called Cdots-AuNC, was designed and constructed on the basis of the assembly of Cdots and AuNCs by a carbodiimide-activated coupling reaction (Fig.…”

Section: Ratiometric Fluorescence Nanoprobesmentioning

confidence: 99%

Ratiometric optical nanoprobes enable accurate molecular detection and imaging

et al. 2018

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Exploring and understanding biological and pathological changes are of great significance for early diagnosis and therapy of diseases. Optical sensing and imaging approaches have experienced major progress in this field. Particularly, an emergence of various functional optical nanoprobes has provided enhanced sensitivity, specificity, targeting ability, as well as multiplexing and multimodal capabilities due to improvements in their intrinsic physicochemical and optical properties. However, one of the biggest challenges of conventional optical nanoprobes is their absolute intensity-dependent signal readout, which causes inaccurate sensing and imaging results due to the presence of various analyte-independent factors that can cause fluctuations in their absolute signal intensity. Ratiometric measurements provide built-in self-calibration for signal correction, enabling more sensitive and reliable detection. Optimizing nanoprobe designs with ratiometric strategies can surmount many of the limitations encountered by traditional optical nanoprobes. This review first elaborates upon existing optical nanoprobes that exploit ratiometric measurements for improved sensing and imaging, including fluorescence, surface enhanced Raman scattering (SERS), and photoacoustic nanoprobes. Next, a thorough discussion is provided on design strategies for these nanoprobes, and their potential biomedical applications for targeting specific biomolecule populations (e.g. cancer biomarkers and small molecules with physiological relevance), for imaging the tumor microenvironment (e.g. pH, reactive oxygen species, hypoxia, enzyme and metal ions), as well as for intraoperative image guidance of tumor-resection procedures.

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Section: Ratiometric Fluorescence Nanoprobesmentioning

confidence: 99%

Ratiometric optical nanoprobes enable accurate molecular detection and imaging

et al. 2018

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Highly Sensitive Fluorescent pH Microsensors Based on the Ratiometric Dye Pyranine Immobilized on Silica Microparticles

Chandra

Prasad

Iuele

et al. 2021

Chemistry A European J

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Pyranine (HPTS) is a remarkably interesting pH sensitive dye that has been used for plenty of applications. Its high quantum yield and extremely sensitive ratiometric fluorescence against pH change makes it a very favorable for pH sensing applications and development of pH nano/microsensors. However, its strong negative charge and lack of easily modifiable functional groups makes it difficult to be used with charged substrates such as silica. This study reports a noncovalent HPTS immobilization methodology on silica microparticles that considers the retention of pH sensitivity as well as long term stability of the pH microsensors. The study emphasizes on importance of surface charge for governing the sensitivity of the immobilized HPTS dye molecules on silica microparticles. Importance of methodology of immobilization that preserves the sensitivity as well as stability of the microsensors is also assessed.

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