Dual Excitatory and Smooth Muscle-relaxing Effect of Sideritis montana Extract on Guinea-pig Ileum

Abstract

The neuronal and smooth muscle effects of a methanol extract prepared from the air-dried flowering aerial parts of Sideritis montana L. (SME) was tested in vitro on Guinea-pig ileum. The chemical composition of the investigated extract was analysed by HPLC-MS, and chrysoeriol, chlorogenic acid and caffeic acid were detected as main constituents. The isolated organ assay showed that S. montana extract caused an immediate contraction and a more slowly developing inhibitory response in the ileum. The SME-induced contractions were strongly inhibited by the acetylcholine muscarinic receptor antagonist atropine (0.5 μM), but not by either the Na+ channel blocker tetrodotoxin (TTX; 0.5 μM) or the histamine H1 receptor antagonist chloropyramine (0.5 μM). Selective desensitization of capsaicin-sensitive neurons by the sensory neuron stimulant and blocker capsaicin did not influence the contractile effect of SME. As to the spasmolytic effect, SME inhibited the effects of electrical field stimulation, exogenous acetylcholine, and histamine. These smooth muscle-relaxing effects were reversible in 40 min by repeated renewals of the bathing solution.

Ethanolic Extracts of Adlay Testa and Hull and Their Active Biomolecules Exert Relaxing Effect on Uterine Muscle Contraction through Blocking Extracellular Calcium Influx in Ex Vivo and In Vivo Studies

“…Chrysoeriol (luteolin 3 -methyl ether), a bioactive flavonoid, is found in several tropical, medicinal plants known for potent antioxidant [36], anti-inflammatory, antitumor, antimicrobial, and antiviral activity. In the literature, some reports show selective bronchodilator effect by K + -induced contractions in the trachea and aorta [37], as well as smooth muscle-relaxing effects [38]. No data to date are available regarding the question of whether 3,5,6,7,8,3 ,4 -heptamethoxyflavone inhibits contractility on uterine smooth muscle.…”

Screening of Volatile Compounds, Traditional and Modern Phytotherapy Approaches of Selected Non-Aromatic Medicinal Plants (Lamiaceae, Lamioideae) from Rtanj Mountain, Eastern Serbia

“…It is mainly used orally as tea for relieving cough associated with a cold, for reducing fever, against stomach ailments, as an antihysteric, tonic, and stimulant, and is used externally to treat wounds ( Table 2 ). The scientifically proven activities are antioxidant, antimicrobial, anti-inflammatory, smooth muscle-relaxing, anti-proliferative, and cytotoxic activities [ 17 , 24 , 25 , 26 , 27 , 28 , 29 ]. These results support the traditional use of S. montana for the healing and prevention of many diseases of modern times.…”

“…These results support the traditional use of S. montana for the healing and prevention of many diseases of modern times. Serbia: wound healing herb (aerial parts)/decoction, infusion/internally [9] Smooth muscle-relaxing methanol extract [27] Bulgaria: for relief of cough associated with cold ns [33] Antiproliferative (cervical cancer) and cytotoxic (melanoma, breast adenocarcinoma and human colon cancer) ethanol, methanol, hexane and ethyl acetate extracts, essential oil [17,22] Spain: digestive ns/infusion/internally [34] Antiinflammatory methanol extract [28] ns-not specified.…”

“…Compounds isolated from various extracts of M. peregrinum are β-sitosterol, labdane diterpenoids (peregrinin, preperegrinin, peregrinol, marrubiin, premarrubiin, cyllenin, and 15-epi-cyllenin A), and phenolic compounds flavone aglycones, flavone glycosides, coumaroylated flavone glycosides, and acteoside-related phenyl-ethanoids (ladanein, 6-hydroxy-5,7,4 -trimetoxyflavone and 5,6,7,4 -tetramethoxyflavone, apigenin, kaemferol, apigenin-7-glucoside, luteolin-7-glucoside, and acteoside) [84]. (33), 93 (32), 107 (28), 147 (27), 105 (26), 109 (19), 79 (19). NI-2: 107(100), 132 (53), 91 (34), 105 (33), 119 (29), 133 (28), 125 (28), 122 (25), 93 (21), 41 (20).…”

Effect of crystallization of caffeic acid enhanced stability and dual biological efficacy

“…In our lab, we routinely check the phytochemicals and computationally design their newer derivatives which ultimately may become suitable candidates for the treatment of Alzheimer's disease and metabolic disorder like cancer. Earlier studies on caffeic acid showed its mild stimulant effect (Liszt, Eder, Wendelin, & Somoza, 2015;Shin et al, 2015;Toth et al, 2015). The past studies also showed its cytoprotective effect (Lee et al, 2015;Nunes et al, 2015), antiangiogenic effect, and moderate toxicity (Akyol et al, 2015;Kosova, Kurt, Olmez, Tuğlu, & Arı, 2015) that can be affected due to the stability of the compounds.…”