Dual-enzymatically crosslinked hyaluronic acid hydrogel as a long-time 3D stem cell culture system

Abstract

Stem cell-based tissue engineering shows enormous potential for regenerative medicine. Threedimensional (3D) stem cell culture is the most basic aspect of tissue engineering. However, achievement of a perfect scaffold for highly efficient 3D cell culture is currently still limited. Herein, a new hyaluronic acid hydrogel dual-enzymatically crosslinked by horseradish peroxidase and choline oxidase is developed as a 3D stem cell culture system. This hydrogel possesses superior stability over two months, controllable biodegradability with hyaluronidases, a high swelling ratio exceeding 6000%, and excellent cytocompatibility in vitro and biocompatibility in vivo. More importantly, a long-time and highly cellular activity 3D culture of bone marrow-derived mesenchymal stem cells was achieved in vitro over 20 days. All these encouraging results highlight the great potential of this new hydrogel for 3D culture and tissue engineering.

Injectable and Antioxidative HT/QGA Hydrogel for Potential Application in Wound Healing

“…Tyramine was grafted onto the HA chain to obtain HT conjugate according to our previous method [ 16 ]. Briefly, the HA sodium salt was dissolved in 100 mL of MES solution.…”

“…HT has already been developed as a protein drug delivery system [ 12 , 13 ], and is widely used in tissue engineering [ 14 , 15 ]. It offers the advantages of cell compatibility, biodegradability, and non-immunogenicity [ 16 ]. Chitosan (CS) is a natural polysaccharide prepared by the deacetylation of chitin [ 17 , 18 ].…”

Advancements in Hydrogel-Based Drug Sustained Release Systems for Bone Tissue Engineering

“…The porous structure of the hydrogel enables substance exchange and cell entrance at the initial stage as well as vascular ingrowth in the follow-up stage. It has been substantially shown that cells are easily suspended within hydrogels, and the viability of the encapsulated cells is highly preserved (Gao et al, 2020;Paez et al, 2020).…”

In vitro three-dimensional cell cultures for bone sarcomas

“…Scaffold 3D systems consist in a structural support that favours cell adhesion, proliferation, migration, cell-to-cell interaction and signalling [33] , [34] . Natural scaffolds are based on molecules that are present in the ECM such as collagen, gelatine and derivatives [35] , [36] , complex matrix (e.g., commercial Matrigel™) and hydrogels [37] , [38] , and polysaccharides as alginate, chitosan or hyaluronic acid [39] , [40] , [41] , [42] , [43] . Whereas the main advantage of these natural scaffolds is their biocompatibility, their production and inter-batches variability are the main issues of these materials.…”