Dual DNA binding specificity of a petal epidermis-specific MYB transcription factor (MYB.Ph3) from Petunia hybrida.

Solano, Roberto; Nieto, Concha; Ávila, Julio; Cañas, Luis A.; Dı́az, Isabel; Paz‐Ares, Javier

doi:10.1002/j.1460-2075.1995.tb07166.x

Cited by 219 publications

(172 citation statements)

References 64 publications

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“…Second, CCAl probes can only detect a single gene on a DNA gel blot even at low stringency and using a probe including the myb-related sequence. In addition, the DNA region to which it binds, and which is conserved in a number of plant Lhcb genes, does not include the plant consensus sequence CCT/AACC for recognition by the maize Myb P protein (Grotewold et al, 1994), the recognition sequence for other plant Myb proteins (e.g., see Solano et al, 1995), or consensus sequences determined for Myb proteins from other kingdoms (TAACTG) (Tanikawa et al, 1993). We conclude, therefore, that CCAl is a nove1 myb-related gene rather than a member of the myb gene family in Arabidopsis.…”

Section: Discussionmentioning

confidence: 99%

A Myb-related transcription factor is involved in the phytochrome regulation of an Arabidopsis Lhcb gene.

et al. 1997

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We have isolated the gene for a protein designated CCAl. This protein can bind t o a region of the promoter of an Arabidopsis light-harvesting chlorophyll a/b protein gene, Lhcb7*3, which is necessary for its regulation by phytochrome. The CCAl protein interacted with two imperfect repeats in the Lhcb7*3 promoter, AAA/,AATCT, a sequence that is conserved in Lhcb genes. A region near the N terminus of CCA1, which has some homology t o the repeated sequence found in the DNA binding domain of Myb proteins, is required for binding t o the Lhcb7*3 promoter. Lines of transgenic Arabidopsis plants expressing antisense RNA for CCAl showed reduced phytochrome induction of the endogenous Lhcb7*3 gene, whereas expression of another phytochrome-regulated gene, rbcS-7A, which encodes the small subunit of ribulose-1 ,Bbisphosphate carboxylase/oxygenase, was not affected. Thus, the CCAl protein acts as a specific activator of Lhcb7*3 transcription in response t o brief red illumination. The expression of CCAl RNA was itself transiently increased when etiolated seedlings were transferred t o light. We conclude that the CCAl protein is a key element in the functioning of the phytochrome signal transduction pathway leading t o increased transcription of this Lhcb gene in Arabidopsis.

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Section: Discussionmentioning

confidence: 99%

A Myb-related transcription factor is involved in the phytochrome regulation of an Arabidopsis Lhcb gene.

et al. 1997

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“…Gene encoding Chalcone synthase (CHS) was activated by another MYB protein from Petunia, PhMYB3, which was expressed specifically in petal epidermis where anthocyanin pigment was made (Solano et al 1995). MYB protein also served to regulate other branches of phenylpropanoid metabolism.…”

Section: Phenylpropanoid Metabolismmentioning

confidence: 99%

MYB transcription factor genes as regulators for plant responses: an overview

Ambawat

Sharma

Yadav

et al. 2013

Physiol Mol Biol Plants

822

544

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Regulation of gene expression at the level of transcription controls many crucial biological processes. Transcription factors (TFs) play a great role in controlling cellular processes and MYB TF family is large and involved in controlling various processes like responses to biotic and abiotic stresses, development, differentiation, metabolism, defense etc. Here, we review MYB TFs with particular emphasis on their role in controlling different biological processes. This will provide valuable insights in understanding regulatory networks and associated functions to develop strategies for crop improvement.

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“…The oligonucleotide mixtures (RP or ACP) were made double-stranded by annealing with primer F (5Ј-CGC-GAA-TTC-TGC-AGG-ATC-C-3Ј) and using the Klenow fragment of DNA polymerase I to synthesize the other strand. Primers F and R (5Ј-TCG-ACT-CGA-GTC-GAG-ATC-G-3Ј) were used for PCR labelling and amplification during random binding site selection (RBSS) as described in Solano et al (1995).…”

Section: Oligonucleotides For the Random Binding Site Selection (Rbss)mentioning

confidence: 99%

Two bZIP proteins from Antirrhinum flowers preferentially bind a hybrid C‐box/G‐box motif and help to define a new sub‐family of bZIP transcription factors

et al. 1998

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SummaryTwo genes encoding bZIP proteins are expressed in flowers of Antirrhinum majus, predominantly in vascular tissues, carpels and anthers. The sequences of cDNA clones encoding these proteins show them to belong to a distinct subfamily of bZIP proteins which also includes LIP19 from rice and MLIPI5 and OBF1 from maize. The sub-family is characterized by the inclusion of very small proteins consisting of essentially a basic domain and a long leucine zipper. Members also have a conserved upstream open reading frame (uORF) in their 5Ј leader sequences, implying a common mode of post-transcriptional control. In vitro, the Antirrhinum bZIP proteins preferentially bind to a novel hybrid C-box/G-box motif which is found in the promoters of some plant histone genes and of some nuclear-encoded genes with plastidial protein products. Expression of the bZIP proteins in transgenic tobacco under control of the CaMV 35S promoter supports the view that they can regulate expression of genes which contain the preferred target motif within their regulatory sequences, although both enhancement and repression of transcript levels of target genes were observed, indicating that the bZIP proteins probably interact with other factors to modulate transcription in different ways, as has been observed for the small MAF family of bZIP proteins in vertebrates.

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Dual DNA binding specificity of a petal epidermis-specific MYB transcription factor (MYB.Ph3) from Petunia hybrida.

Cited by 219 publications

References 64 publications

A Myb-related transcription factor is involved in the phytochrome regulation of an Arabidopsis Lhcb gene.

A Myb-related transcription factor is involved in the phytochrome regulation of an Arabidopsis Lhcb gene.

MYB transcription factor genes as regulators for plant responses: an overview

Two bZIP proteins from Antirrhinum flowers preferentially bind a hybrid C‐box/G‐box motif and help to define a new sub‐family of bZIP transcription factors

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