Dual delivery of tuberculosis drugs via cyclodextrin conjugated curdlan nanoparticles to infected macrophages

Basha, Rubaiya Yunus; Kumar, T.S. Sampath; Doble, Mukesh

doi:10.1016/j.carbpol.2019.04.056

Cited by 68 publications

(29 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Both ZM and ZMA (Figure S5) were not cytotoxic to Raw 264.7 cells, and the cell viability was above 90%, which could be considered nontoxic according to ISO 10993-5 . This is in line with other biocompatibility studies reported with other bacterial glucans ,, and yeasts …”

Section: Resultssupporting

confidence: 85%

Synthesis, Characterization, and Biological Activity of Aminated Zymosan

2020

Self Cite

View full text Add to dashboard Cite

Zymosan (ZM), a naturally occurring insoluble macromolecule obtained from the cell wall of Saccharomyces cerevisiae, is used as a functional food (as dietary fiber), phagocytic stimulus, and immune potentiator. The present study aimed to increase its solubility and evaluate its immunological application. ZM was converted into soluble 6-amino-6-deoxy-β-(1-3)-glucan of a molecular weight of 296 kDa by reduction. Detailed structural characterization of aminated ZM was determined by Fourier transform infrared spectroscopy and two-dimensional NMR analysis (2D, COSY, TOCSY, ROSEY, NOSEY, and HSQC). Aminated ZM was biocompatible with Raw 264.7 macrophage cell lines up to a concentration of 100 μg/mL. Rhodamine tagging revealed that the aminated ZM microparticles were found localized within the nucleus of Raw 264.7 cells. Both native and aminated ZM showed a similar expression pattern of inflammatory genes in Raw 264.7.

show abstract

Section: Resultssupporting

confidence: 85%

Synthesis, Characterization, and Biological Activity of Aminated Zymosan

2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…The cross-linking density of curdlan matrices is a prevalent factor determining the drug release rates and mechanism [ 53 ]. Therefore, more densely cross-linked curdlan films may find application as a vehicle for sustained release of the drugs over a prolonged period of time, while being more resistant to enzymatic degradation [ 54 , 55 ]. In turn, curdlan matrices with lower cross-linking densities can be applied in the treatment of acute invasive infections, causing a burst release of a large dose of the drug.…”

Section: Discussionmentioning

confidence: 99%

Effect of Gelation Temperature on the Molecular Structure and Physicochemical Properties of the Curdlan Matrix: Spectroscopic and Microscopic Analyses

Gieroba

Sroka-Bartnicka

Kazimierczak

et al. 2020

IJMS

View full text Add to dashboard Cite

In order to determine the effect of different gelation temperatures (80 °C and 90 °C) on the structural arrangements in 1,3-β-d-glucan (curdlan) matrices, spectroscopic and microscopic approaches were chosen. Attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) and Raman spectroscopy are well-established techniques that enable the identification of functional groups in organic molecules based on their vibration modes. X-ray photoelectron spectroscopy (XPS) is a quantitative analytical method utilized in the surface study, which provided information about the elemental and chemical composition with high surface sensitivity. Contact angle goniometer was applied to evaluate surface wettability and surface free energy of the matrices. In turn, the surface topography characterization was obtained with the use of atomic force microscopy (AFM) and scanning electron microscopy (SEM). Described techniques may facilitate the optimization, modification, and design of manufacturing processes (such as the temperature of gelation in the case of the studied 1,3-β-d-glucan) of the organic polysaccharide matrices so as to obtain biomaterials with desired characteristics and wide range of biomedical applications, e.g., entrapment of drugs or production of biomaterials for tissue regeneration. This study shows that the 1,3-β-d-glucan polymer sample gelled at 80 °C has a distinctly different structure than the matrix gelled at 90 °C.

show abstract

“…These molecules are able to form inclusion complexes with a variety of lipophilic molecules and are used for many pharmaceutical applications [22]. Besides, βCD have become commonly employed in nanoparticle-based drug delivery systems thanks to their benefits [23,24,25] and some nanovehicles based on βCD-PTX complexes have been already describe in literature [26,27].…”

Section: Introductionmentioning

confidence: 99%

Paclitaxel-Trastuzumab Mixed Nanovehicle to Target HER2-Overexpressing Tumors

et al. 2019

View full text Add to dashboard Cite

Paclitaxel is one of the most widely used chemotherapeutic agents thanks to its effectiveness and broad spectrum of antitumor activity. However, it has a very poor aqueous solubility and a limited specificity. To solve these handicaps, a novel paclitaxel-trastuzumab targeted transport nanosystem has been developed and characterized in this work to specifically treat cancer cells that overexpress the human epidermal growth factor receptor-2 (HER2). Methods: Alginate and piperazine nanoparticles were synthetized and conjugated with paclitaxel:β-cyclodextrins complexes and trastuzumab. Conjugated nanoparticles (300 nm) were characterized and their internalization in HER2-overexpressing tumor cells was analyzed by immunofluorescence. Its specific antitumor activity was studied in vitro using human cell lines with different levels of HER2-expression. Results: In comparison with free paclitaxel:β-cyclodextrins complexes, the developed conjugated nanovehicle presented specificity for the treatment of HER2-overpressing cells, in which it was internalized by endocytosis. Conclusions: It seems that potentially avoiding the conventional adverse effects of paclitaxel treatment could be possible with the use of the proposed mixed nanovehicle, which improves its bioavailability and targets HER2-positive cancer cells.

show abstract

Dual delivery of tuberculosis drugs via cyclodextrin conjugated curdlan nanoparticles to infected macrophages

Cited by 68 publications

References 55 publications

Synthesis, Characterization, and Biological Activity of Aminated Zymosan

Synthesis, Characterization, and Biological Activity of Aminated Zymosan

Effect of Gelation Temperature on the Molecular Structure and Physicochemical Properties of the Curdlan Matrix: Spectroscopic and Microscopic Analyses

Paclitaxel-Trastuzumab Mixed Nanovehicle to Target HER2-Overexpressing Tumors

Contact Info

Product

Resources

About