Dual Coating Improves the Survival of Probiotic Bifidobacterium Strains during Exposure to Simulated Gastro-Intestinal Conditions

Abstract

Probiotics have been reported to benefit human health by modulating immunity, lowering cholesterol, improving lactose tolerance, and preventing some cancer. Once ingested, probiotic microorganisms have to survive harsh conditions such as low pH, protease-rich condition, and bile salts during their passage through the gastro-intestinal (GI) tract colonize and proliferate to exert their probiotic effects. The dual coating technology, by which the bacteria are doubly coated with peptides and polysaccharides in consecutive order, was developed to protect the ingested bacteria from the harsh conditions. The aim of the study was to evaluate the viable stability of a doubly coated blend of four species of Bifidobacterium by comparing its bile/acid resistance and heat viability in vitro with that of the non-coated blend. After challenges with acid, bile salts, heat, and viable cell counts (VVCs) of the dual coated and non-coated blend were determined by cultivation on agar plates or flow cytometric measurement after being stain with the BacLigtht kit TM . The results showed that the dual coated blend was much higher resistant to the acidic or bile salt condition than the non-coated blend and heat viability was also higher, indicating that the dual coating can improve the survival of probiotic bacteria during their transit through the GI tract after consumption.

Hepatoprotective effects of dual-coated and uncoated mixture of probiotics in rats

“…[14] Another recent in vivo and in vitro study showed that dual-coated LAB (lactic-acid bacteria) have stronger probiotic effects compared to uncoated LAB. [21] In in vivo experiments, double-coated live L. plantarum KCTC3928 has demonstrated hypocholesterolaemic effects in mice.…”

“…The dualcoating offers better protection from heat, pH conditions and moisture. [14,15] Thus, the stability of dual-coated probiotics is increased in the gastrointestinal environment. The purpose of the present study was to examine the hepatoprotective effects of dual-coated probiotics and uncoated probiotics on CCl 4 -induced acute hepatic injury in rats.…”

Double layered encapsulation to immobilize Bifidobacterium bifidum ATCC 35914 in polysaccharide‐protein matrices and their viability in set type yoghurt

“…These are also supposed to effectively maintain the viability of probiotics in acidic foods as well as utilize the whey (dairy waste) precisely (Iqbal et al, 2019;Kang et al, 2013). The current study was designed to compare the protective effect of coating materials on the survival of B. bifidum and fate of double-coated bacteria in set type yoghurt during storage.…”

“…The applications of aforementioned coating materials for single and double coatings of probiotics are the need of the day. These are also supposed to effectively maintain the viability of probiotics in acidic foods as well as utilize the whey (dairy waste) precisely (Iqbal et al, 2019; Kang et al, 2013). The current study was designed to compare the protective effect of coating materials on the survival of B .…”