Dual cell seeding and the use of zymogen tissue plasminogen activator to improve cell retention on polytetrafluoroethylene grafts

Yu, Hong; Wang, Ying; Eton, Darwin; Rowe, Vincent L.; Terramani, Thomas T.; Cramer, Donald V.; Starnes, Vaughn A.

doi:10.1067/mva.2001.114817

Cited by 14 publications

(21 citation statements)

References 34 publications

(35 reference statements)

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“…This property is important for cell seeding on grafts to deliver therapeutic proteins. Our previous studies 16,20 demonstrated that VSMC can improve endothelial cell retention on polytetrafluoroethylene grafts in vivo. A stent graft suffused with engineered VSMC is a potential vehicle for therapeutic gene delivery into the blood stream.…”

Section: Discussionmentioning

confidence: 99%

“…Plasmid pLPCSEAP was generated by inserting the Hind III/Xba I fragment of pSEAP2 Basic (Clontech, Mountain View, Calif) into vector pLPCX (Clontech) Hind III/Avr II sites. Viral vector supernatants with titers of 1 -5 ϫ 10 6 colony forming unit per ml (cfu/ml) were generated as previously described 20 from producer cell lines 293/GPG/ G1nBgSvNa and 293/GPG/pLPCSEAP for transfer of genes encoding for nuclear-localized ␤-galactosidase (␤-gal) and SEAP, respectively. Transduction of BD-SMC and VSMC with viral vectors G1nBgSvNa and LPCSEAP was performed using the multiplicity of infection (MOI) of approximate 100.…”

Section: Methodsmentioning

confidence: 99%

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Blood-derived smooth muscle cells as a target for gene delivery

Yang

Shao

Tan

et al. 2008

Journal of Vascular Surgery

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Blood-derived smooth muscle cells as a target for gene delivery

Yang

Shao

Tan

et al. 2008

Journal of Vascular Surgery

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“…Since endothelial cells form only monolayers [31], a thick vascular tissue capable of withstanding stent deployment and capable of regenerating after stent deployment may not be able to form using only HUVECs. One potential solution involves first adding smooth muscle cells to the complex-shaped scaffolds followed by endothelial cells, since smooth muscle cells have been shown to improve endothelial cell attachment to synthetic grafts [8,19,32–34]. Another potential solution involves adding circulating endothelial progenitor cells to the bioreactor system since they have been shown to accelerate re-endothelialization [35–37] or adding vascular-resident endothelial progenitor cells since current findings support their involvement in tissue regeneration processes [38].…”

Section: Discussionmentioning

confidence: 99%

Tissue-engineered blood vessel mimics in complex geometries for intravascular device testing

2019

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Objective Intravascular stents are commonly used to treat occluded arteries during coronary heart disease. After coronary stent implantation, endothelial cells grow over the stent, which is referred to as re-endothelialization. Re-endothelialization prevents blood from clotting on the stent surface and is a good predictor of stent success. Blood vessel mimics (BVMs) are in vitro tissue-engineered models of human blood vessels that may be used to preclinically test stents for re-endothelialization. BVMs have been developed in straight geometries. However, the United States Food and Drug Administration recommends that devices intended to treat coronary occlusions be preclinically tested in bent and bifurcated vessels due to the complex geometries of native coronary arteries. The main objectives of this study were to develop and characterize BVMs in complex geometries. Design Bioreactors were designed and constructed so that BVMs could be cultivated in bent (>45°) and bifurcated geometries. Human umbilical vein endothelial cells were sodded onto complex-shaped scaffolds, and the resulting BVMs were characterized for cell deposition. For a final proof of concept, a coronary stent was deployed in a severely angulated BVM. Results The new bioreactors were easy to use and mounting scaffolds in complex geometries in the bioreactors was successful. After sodding scaffolds with cells, there were no statistically significant differences between the cell densities along the length of the BVMs, on the top and bottom halves of the BVMs, or on the inner and outer halves of the BVMs. This suggests cells deposited evenly throughout the scaffolds, resulting in consistent complex-geometry BVMs. Also, a coronary stent was successfully deployed in a severely angulated BVM. Conclusions Bioreactors can be constructed for housing complex-shaped vessels. BVMs can be developed in the complex geometries observed in native coronary arteries with endothelial cells evenly dispersed throughout BVM lumens.

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“…Finally focal adhesions or contacts are formed, consisting of clustered integrins and other transmembrane, membrane-associated, and cytosolic molecules, which link ECM molecules to the cell's actin cytoskeleton (38). It is notable that previous studies have shown that a layer of SMCs itself enhances the retention of ECs (21,39).…”

Section: Discussionmentioning

confidence: 99%

Tissue engineering of a hybrid bypass graft for coronary and lower limb bypass surgery

et al. 2008

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Tissue-engineered blood vessels have largely relied on inelastic scaffolds or biological solutions with uncertain long-term in vivo durability. In this report we present for the first time a hybrid tissue-engineered bypass graft consisting of an elastic scaffold of compliant poly(carbonate-urea)urethane (CPU), incorporated with human smooth muscle cells (SMCs) and endothelial cells (ECs) from the same human source. Human vascular SMCs and ECs were extracted from umbilical cord vessels. The effect of shear stress preconditioning on cell retention on the hybrid bypass graft was investigated under pulsatile arterial flow conditions. Retention of ECs seeded onto CPU precoated with SMCs was significantly improved by a period of shear stress preconditioning, especially when the stress incrementally increased. This is probably because the mechanical stimuli orient cells and increase the release of matrix proteins and attachment factors. The stage is now set for developing a hybrid graft for in vivo studies.

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Dual cell seeding and the use of zymogen tissue plasminogen activator to improve cell retention on polytetrafluoroethylene grafts

Abstract: SMC seeded between EC and PTFE improves EC retention in vitro. Transduction of zymogen tPA increases thrombolytic ability of seeded cells with less adverse impact on cell retention than wild-type tPA.

Cited by 14 publications

References 34 publications

Blood-derived smooth muscle cells as a target for gene delivery

Blood-derived smooth muscle cells as a target for gene delivery

Tissue-engineered blood vessel mimics in complex geometries for intravascular device testing

Tissue engineering of a hybrid bypass graft for coronary and lower limb bypass surgery

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