Blood-derived smooth muscle cells as a target for gene delivery

Yang, Zhe; Shao, Hongwei; Tan, Yaohong; Eton, Darwin; Yu, Hong

doi:10.1016/j.jvs.2007.10.039

Cited by 6 publications

(7 citation statements)

References 34 publications

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“…Assays of cell proliferation and apoptosis were performed on ASCs/Sox‐9 after 14 days of alginate bead culture. The methods used was previously described 23. For the cell proliferation assay, ASCs/Sox‐9 were released from alginate beads, and re‐plated at a density of 1 × 10 4 cells per well in a 24‐well plate.…”

Section: Methodsmentioning

confidence: 99%

Sox‐9 facilitates differentiation of adipose tissue‐derived stem cells into a chondrocyte‐like phenotype in vitro

Yang

Huang

Candiotti

et al. 2011

Journal Orthopaedic Research

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The purpose of this study is to test whether ectopic expression of Sox-9 can induce adipose tissue-derived stem cells (ASCs) to function as real nucleus pulposus (NP) cells in vitro. Adenoviral vectors expressing Sox-9 were reported to infect the chondroblastic and human disc cells, which resulted in increased Sox-9 and type II collagen production. ASCs were isolated from rat inguinal adipose pad, characterized, and transduced in vitro with a retroviral vector encoding the Sox-9 gene. Sox-9-engineered ASCs (ASCs/Sox-9) were induced for the chondrocyte-like cell differentiation by 3D cultured in alginate beads and TGF-b3 for 2 weeks. Expression of exogenous Sox-9 protein was detected. Type II collagen and Aggrecan gene expressions of induced ASCs/Sox-9 were measured using real-time PCR; proteoglycans expressions were measured by checking the glycosaminoglycan content and type II collagen production by enzyme-linked immunosorbent assay. Isolated ASCs were CD 29À . ASCs/Sox-9 expressed marked increase in exogenous Sox-9 protein. After induction, type II collagen gene expression was sevenfold higher in mRNA levels, with an approximately twofold increase in protein levels of ASCs/Sox-9 compared to ASCs. Type II collagen and proteoglycan productions were significantly increased in the ASCs/Sox-9 compared to the ASCs. In addition, co-culture of induced ASCs/Sox-9 with matured NP cells resulted in enhanced increase in proteoglycan and type II collagen production. Constitutive retroviral expression of Sox-9 could efficiently induce ASCs differentiation into chondrocytelike cells. This novel approach may provide a practicable system for a simple and rapid differentiation of ASCs into chondrocyte-like cells which may be potentially used as a stem cell-based therapeutic tool for the treatment of degenerative disc diseases. ß

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Section: Methodsmentioning

confidence: 99%

Sox‐9 facilitates differentiation of adipose tissue‐derived stem cells into a chondrocyte‐like phenotype in vitro

Yang

Huang

Candiotti

et al. 2011

Journal Orthopaedic Research

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“…Mononuclear cells isolated from peripheral blood have been differentiated into blood-derived smooth muscle cells. 31 Blood-derived smooth muscle cells have similar characteristics to vascular-derived smooth muscle cells and have been successfully transfected with a retroviral vector, raising the potential for ex vivo genetic engineering. Other investigators have transferred recombinant tropoelastin to aortic vascular smooth muscle cells of rats with abdominal aortic aneurysms.…”

Section: Gene Therapymentioning

confidence: 99%

Translational Research in Surgical Disease

et al. 2010

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“…9,10 BOECs are easily grown out from peripheral blood mononuclear cells (PBMCs) emerging as well-defined colonies after 7-21 days in culture. [13][14][15][16][17] It is well known that vascular cells interact with leukocytes to amplify inflammatory responses. Similarly, although less well described, there is a smooth muscle cell type which can also be grown out from blood cells.…”

Section: Introductionmentioning

confidence: 99%

“…Similarly, although less well described, there is a smooth muscle cell type which can also be grown out from blood cells . In this way, blood outgrowth smooth muscle cells (BO‐SMCs) have the potential to provide the same value as BOECs in vascular cell research …”

Section: Introductionmentioning

confidence: 99%

“…12 In this way, blood outgrowth smooth muscle cells (BO-SMCs) have the potential to provide the same value as BOECs in vascular cell research. [13][14][15][16][17] It is well known that vascular cells interact with leukocytes to amplify inflammatory responses. An important example in drug testing is the fact that cytokine storm responses to biological drugs such as the CD28 superantigen, TGN1412, in aqueous phase require leukocytes and an endothelial interface.…”

Section: Introductionmentioning

confidence: 99%

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A bioassay system of autologous human endothelial, smooth muscle cells, and leukocytes for use in drug discovery, phenotyping, and tissue engineering

et al. 2019

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Blood vessels are comprised of endothelial and smooth muscle cells. Obtaining both types of cells from vessels of living donors is not possible without invasive surgery. To address this, we have devised a strategy whereby human endothelial and smooth muscle cells derived from blood progenitors from the same donor could be cultured with autologous leukocytes to generate a same donor “vessel in a dish” bioassay. Autologous sets of blood outgrowth endothelial cells (BOECs), smooth muscle cells (BO‐SMCs), and leukocytes were obtained from four donors. Cells were treated in monoculture and cumulative coculture conditions. The endothelial specific mediator endothelin‐1 along with interleukin (IL)‐6, IL‐8, tumor necrosis factor α, and interferon gamma‐induced protein 10 were measured under control culture conditions and after stimulation with cytokines. Cocultures remained viable throughout. The profile of individual mediators released from cells was consistent with what we know of endothelial and smooth muscle cells cultured from blood vessels. For the first time, we report a proof of concept study where autologous blood outgrowth “vascular” cells and leukocytes were studied alone and in coculture. This novel bioassay has usefulness in vascular biology research, patient phenotyping, drug testing, and tissue engineering.

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Blood-derived smooth muscle cells as a target for gene delivery

Abstract: BD-SMCs have similar characteristics to mature VSMCs and can be used as a novel target for gene transfer to deliver a therapeutic protein.

Cited by 6 publications

References 34 publications

Sox‐9 facilitates differentiation of adipose tissue‐derived stem cells into a chondrocyte‐like phenotype in vitro

Sox‐9 facilitates differentiation of adipose tissue‐derived stem cells into a chondrocyte‐like phenotype in vitro

Translational Research in Surgical Disease

A bioassay system of autologous human endothelial, smooth muscle cells, and leukocytes for use in drug discovery, phenotyping, and tissue engineering

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