Dual aptamer assay for detection of Acinetobacter baumannii on an electromagnetically-driven microfluidic platform

Su, Chin-Heng; Tsai, Meng‐Li; Lin, Chia-Ying; Ma, Yu-Dong; Wang, Chih‐Hung; Chung, Yi-Da; Lee, Gwo‐Bin

doi:10.1016/j.bios.2020.112148

Cited by 51 publications

(27 citation statements)

References 34 publications

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“…1 a). Prior to analysis, the magnetic beads were coated with RNA probes (amine group modification) ( Table S3 ) by carboxylation with 1-Ethyl-3-(3-Dimethylaminopropyl) carbodiimide catalytic action [ 31 ]. Note that these three RNA probes were specifically designed to be complementary to three target genes (i.e.…”

Section: Methodsmentioning

confidence: 99%

“…Microstructures were designed by AutoCAD 2019 and Solidworks 2014, and the EMC was microfabricated by casting of polydimethylsiloxane (PDMS; 12:1 A:B ratio) onto polymethyl methacrylate (PMMA) mastermolds ( Figs. S5–S6 ) as in a prior work [ 31 ]. To align the permanent magnets, they were first fixed on a PMMA mold, and PDMS was then poured over the mold to position them above the top PDMS layer.…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, most RT-PCR and RT-LAMP assays for detection of SARS-CoV-2 [ [24] , [25] , [26] , [27] , [28] , [29] ] focus on only 1–2 genes, even though a three-gene approach featuring RdRp, E, and N genes (preferably detected simultaneously) [ 30 ] would reduce both false-positive and false-negative diagnoses [ 36 ]. In this work, an electromagnetic microfluidic chip (EMC) adopted from our previous work [ 31 ] equipped with micropumps and micromixers was modified to be equipped a new detachable “magnetic structure layer” herein to control samples and reagents. Moreover, micro temperature control and optical detection modules were further integrated with the electromagnetically-driven microfluidic devices to detect all three genes (RdRp, E, and N) of SARS-CoV-2 in parallel optically on a single chip that could carry out all steps of the viral gene detection process ( Fig.…”

Section: Introductionmentioning

confidence: 99%

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Electromagnetically-driven integrated microfluidic platform using reverse transcription loop-mediated isothermal amplification for detection of severe acute respiratory syndrome coronavirus 2

Tsai

Wang

Tsai

et al. 2022

Analytica Chimica Acta

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Electromagnetically-driven integrated microfluidic platform using reverse transcription loop-mediated isothermal amplification for detection of severe acute respiratory syndrome coronavirus 2

Tsai

Wang

Tsai

et al. 2022

Analytica Chimica Acta

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“…One of the possible, and still more and more popular, is the use of electrochemical aptasensors as detection elements in such. This is especially driven by the microelectronics progress and digitalization of the surrounded world, which led, e.g., to the production of miniaturized handheld point-of-care (PoC) devices [191][192][193][194][195]. Such a medical diagnostic development is of special interest as in an aging society, improvement of quality of life is one of the most important goals.…”

Section: Microfluidic Devices and Point-of-care Testingmentioning

confidence: 99%

“…In turn, this would significantly increase the awareness of the current health status of an individual person, and the same time, it would reduce the time necessary to implement appropriate medical procedures. Such devices, in comparison to traditional laboratory procedures, offer significantly reduced sample volume and shorter detection time, improved sensitivity due to high surface to volume ratio (also surface modified with carefully chosen nanomaterials), high throughput by parallel operation, portability, and disposability and what is no less important also real-time detection and an automated measurement process [191][192][193][194][195].…”

Section: Microfluidic Devices and Point-of-care Testingmentioning

confidence: 99%

From Small Molecules toward Whole Cells Detection: Application of Electrochemical Aptasensors in Modern Medical Diagnostics

Ziółkowski

Jarczewska

Górski

et al. 2021

Sensors

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This paper focuses on the current state of art as well as on future trends in electrochemical aptasensors application in medical diagnostics. The origin of aptamers is presented along with the description of the process known as SELEX. This is followed by the description of the broad spectrum of aptamer-based sensors for the electrochemical detection of various diagnostically relevant analytes, including metal cations, abused drugs, neurotransmitters, cancer, cardiac and coagulation biomarkers, circulating tumor cells, and viruses. We described also possible future perspectives of aptasensors development. This concerns (i) the approaches to lowering the detection limit and improvement of the electrochemical aptasensors selectivity by application of the hybrid aptamer–antibody receptor layers and/or nanomaterials; and (ii) electrochemical aptasensors integration with more advanced microfluidic devices as user-friendly medical instruments for medical diagnostic of the future.

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Quantum Dots for Pathogenic Bacterial Monitoring and Combating

Liu

Zhang

2022

Advanced Optical Materials

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including neonatal meningitis, [6] diarrhoea, [7] infected cutaneous wound [8] or bloodstream diseases, [9][10][11] etc. Antibiotic treatment strategies against these bacteria have been widely applied while antimicrobial resistance has been arousing gradually. [12][13][14][15][16][17] What is worse, the biofilmrelated infection has been a tough issue for bacterial infectious treatment since it is usually chronic and much harder to eradicate. [18][19][20] Therefore, effective antibacterial treatments for drug-resistant pathogenic bacteria are vital problems for clinical therapy. [21] Some anti-microbial trials were carried out like phages, bacteriocins, and antimicrobial peptides [22] along with various therapeutic methods. [23][24][25] Among them, nanomaterials are considered as compatible solutions [23,26] due to their facile synthesis methods and rich-sourced precursors, broad-spectrum sterilization, and low drug resistance. [27][28][29][30][31] Nanomaterials are generally easy to be modified or doped to enhance the affinity toward bacteria for the sake of combating or drug delivery. [32][33][34][35] The antibacterial mechanism [36,37] could be result from the sharp edges-based physical cutting, [38,39] photothermal effects, [40][41][42] and photo dynamic or chemical dynamic properties, that could produce reactive oxygen species (ROS).Pathogenic bacteria monitoring mainly conducted by culturing them in agar-based media, and subsequently testing them with biochemical probes. [43][44][45][46] Although this conventional method enables clinical diagnoses with relatively reliable outcomes, the long culturing time renders the "golden therapeutical window" for patients. So more rapid and accurate detecting technologies have been invented. One of the most evolutionary methods is the polymerase chain reaction, [47][48][49] which can identify the bacterial classification and provide gene expression level in a few hours with better accuracy. [47,50] Besides, the coupling of chromatography and mass spectra like matrix-assisted laser desorption ionization time-of-flight mass spectrometry [51][52][53][54][55] can play a more exact role in diagnosis. [56] Though these advanced instrumental-relied methods can lead to fast diagnostic, the expensive apparatus, environmentally unfriendly reagents, [57] professional sites and technical personnel requirements limited their utilization in real-time monitoring and point-of-care sensing. By contrast, easy and fast monitoring methods for the pathogen, such as electrochemical and fluorescence sensors, were brought out as powerful supplements. [58][59][60][61][62] Therein, Antibiotic resistance and pathogenic bacterial monitoring are two main challenges in clinical diagnostics and treatment of bacteria. To conquer those issues, many new materials and novel technologies are explored. Among them, quantum dots (QDs) are regarded as not only powerful bacteriostatic agents, but also bacterial monitoring probes due to their excellent photoluminescence properties, tunable multiwavelength lumine...

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Dual aptamer assay for detection of Acinetobacter baumannii on an electromagnetically-driven microfluidic platform

Cited by 51 publications

References 34 publications

Electromagnetically-driven integrated microfluidic platform using reverse transcription loop-mediated isothermal amplification for detection of severe acute respiratory syndrome coronavirus 2

Electromagnetically-driven integrated microfluidic platform using reverse transcription loop-mediated isothermal amplification for detection of severe acute respiratory syndrome coronavirus 2

From Small Molecules toward Whole Cells Detection: Application of Electrochemical Aptasensors in Modern Medical Diagnostics

Quantum Dots for Pathogenic Bacterial Monitoring and Combating

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