Isotopic carbon discrimination (Δ 13 C) of winter wheat grain grown under different water and nitrogen supplies was determined. In two field experiments during years 2004-2007 (A) and 2008-2013 (B), a water shortage was induced from the flowering stage on with a mobile shelter (S), while an optimal water supply was ensured with drip irrigation (I), and a rain-fed crop served as the control treatment (R). Water supply had a statistically significant effect on grain Δ 13 C values in both experiments (P < 0.01). The average values of grain Δ 13 C in treatments I, R and S were 19.43, 18.68 and 17.70‰ (A); and 20.36, 19.60 and 18.13‰ (B). Grain Δ 13 C was in a significant linear relationship (P < 0.01) with the amount of water supplied by precipitation or irrigation. The regressions suggested that grain Δ 13 C increased by 1.14‰ and 1.16‰ (A), and 0.98‰ or 0.96‰ (B) for every 100 mm of water from January and March, respectively, until the early dough stage (r = 0.79-0.74, P < 0.05). Pooled data for the whole period 2004-2013 showed increases of 1.06‰ and 1.08‰ (r = 0.91 and 0.82, P < 0.05) for 100 mm of water, respectively. The results of the experiment confirmed the stable and predictable effect of water supply on wheat grain Δ 13 C.
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329Plant Soil Environ. Vol. 62, 2016, No. 7: 329-334 doi: 10.17221/118/2016-PSE was observed, especially when plants that were either under strong stress (semiarid conditions) or under irrigation were compared (Zhu et al. 2009, Misra et al. 2010. The value of Δ 13 C in seeds integrates the effects of water supply and plant water status during growth. However, this assumption about a positive correlation between yield and Δ 13 C has not always been confirmed, as the response to water shortage and the process of yield formation depends on many year-, agronomy-, and site-dependent factors during growth (Monneveux et al. 2005). Under temperate and transition (maritime/inland) climatic conditions, where precipitation during growth is highly variable, rain-out shelters enable a standardization of the water supply in the field, partially independent of that year's meteorological conditions (e.g., Yasir et al. 2013). The aim of the study was to examine the impact of water supply differentiated from anthesis, during the period of wheat grain growth upon values of Δ 13 C, to determine the relationships with water supply during growth and any possible interactive effect of nitrogen fertilization.
MATERIAL AND METHODSTwo successive experiments (A and B) with differentiated water supplies during grain growth were carried out at the Crop Research Institute (CRI), Ruzyně near Prague, Czech Republic (50°05'N, 14°20'W), altitude 340 m a.s.l., average annual sum of precipitation 477 mm, and average daily temperature of 8.5 o C. The experimental field is a fertile deep loamy-clay Haplic Chernozem soil on loess. The contents of available P, K, and Mg (according to Mehlich III) were 85, 245 and 226 mg/kg dry soil in the topsoil (0-30 cm); and 10-12, 121-141 and 254-352 mg/kg in the s...