Dry-heat destruction of lipopolysaccharide: design and construction of dry-heat destruction apparatus

Robertson, J.H.; Gleason, D; Tsuji, K

doi:10.1128/aem.36.5.705-709.1978

Cited by 4 publications

(1 citation statement)

References 16 publications

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“…The cup was then placed under a stream of dry nitrogen for approximately 3 h to dry. The cup containing dried LPS was dry heat treated at various temperatures from 170 to 250°C for various time intervals, using the dry-heat destruction oven described previously (21). After the dry-heat treatment, the cup containing LPS was dropped into a sterile disposable test tube containing at least 5 ml of pyrogen-free water.…”

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confidence: 99%

Dry-heat destruction of lipopolysaccharide: dry-heat destruction kinetics

Tsuji¹,

Harrison²

1978

Appl Environ Microbiol

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Dry-heat destruction kinetics of lipopolysaccharides from Escherichia coli, Serratia marcescens, and Salmonella typhosa at 170 to 250 degrees C are described. The destruction rate seems to follow the second order and can be linearized by the equation, log y = a + b . -10cx. Because c is the slope, 1/c = D3. Both a and b are constant at a given temperature and are linear functions of temperature. The D(3)170, D(3)190, D(3)210, D(3)230, and D(3)250 values for E. coli lipopolysaccharide are 251, 99.4, 33.3, 12.3, and 4.99 min, respectively, with a z value of 46.4 min. The D values for lipopolysaccharides from S. marcescens and S. typhosa are not significantly different from those from E. coli lipopolysaccharide.

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Section: Samentioning

confidence: 99%

Dry-heat destruction of lipopolysaccharide: dry-heat destruction kinetics

Tsuji¹,

Harrison²

1978

Appl Environ Microbiol

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Thermal effects on bacterial bioaerosols in continuous air flow

Jung

Lee

Kim

2009

Science of The Total Environment

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Inactivation of Escherichia coli Endotoxin by Soft Hydrothermal Processing

Miyamoto

Okano

Kasai

2009

Appl Environ Microbiol

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Bacterial endotoxins, also known as lipopolysaccharides, are a fever-producing by-product of gram-negative bacteria commonly known as pyrogens. It is essential to remove endotoxins from parenteral preparations since they have multiple injurious biological activities. Because of their strong heat resistance (e.g., requiring dry-heat sterilization at 250°C for 30 min) and the formation of various supramolecular aggregates, depyrogenation is more difficult than sterilization. We report here that soft hydrothermal processing, which has many advantages in safety and cost efficiency, is sufficient to assure complete depyrogenation by the inactivation of endotoxins. The endotoxin concentration in a sample was measured by using a chromogenic limulus method with an endotoxin-specific limulus reagent. The endotoxin concentration was calculated from a standard curve obtained using a serial dilution of a standard solution. We show that endotoxins were completely inactivated by soft hydrothermal processing at 130°C for 60 min or at 140°C for 30 min in the presence of a high steam saturation ratio or with a flow system. Moreover, it is easy to remove endotoxins from water by soft hydrothermal processing similarly at 130°C for 60 min or at 140°C for 30 min, without any requirement for ultrafiltration, nonselective adsorption with a hydrophobic adsorbent, or an anion exchanger. These findings indicate that soft hydrothermal processing, applied in the presence of a high steam saturation ratio or with a flow system, can inactivate endotoxins and may be useful for the depyrogenation of parenterals, including end products and medical devices that cannot be exposed to the high temperatures of dry heat treatments.

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Dry-heat destruction of lipopolysaccharide: design and construction of dry-heat destruction apparatus

Cited by 4 publications

References 16 publications

Dry-heat destruction of lipopolysaccharide: dry-heat destruction kinetics

Dry-heat destruction of lipopolysaccharide: dry-heat destruction kinetics

Thermal effects on bacterial bioaerosols in continuous air flow

Inactivation of Escherichia coli Endotoxin by Soft Hydrothermal Processing

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