Drug resistance in a non-hemolytic Streptococcus sp. isolated from cultured yeltowtail Seriola quinqueradiata

Aoki, Takashi; Takami, Kazutoshi; Kitao, Tadatoshi

doi:10.3354/dao008171

Cited by 38 publications

(44 citation statements)

References 5 publications

(4 reference statements)

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“…Macrolide antimicrobials are not approved for administrating to Japanese flounder in Japan, but they have been used for treating streptococcicosis causing by Lactococcus garvieae in yellowtail Seriola quinqueradiata for many years. Several published works showed that L. garvieae strains isolated from yellowtail carried transferable R-plasmid on which erm(B) and tet (S) were integrated (Aoki et al, 1990;Maki et al, 2008). In addition, L. garvieae is also one of the pathogens causing streptococcicosis in cultured Japanese flounder (Fukuda, 2003).…”

Section: Discussionmentioning

confidence: 99%

Characterization of Drug Resistance in Streptococcus parauberis Isolated from Japanese Flounder

2009

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ABSTRACT-Antimicrobial susceptibility of Streptococcus parauberis against nine drugs were investigated in 64 strains isolated from Japanese flounder Paralichthys olivaceus at different culture areas of western Japan, and resistance genes were determined by PCR and Southern blot hybridization. Five of the tested strains belonging to S. parauberis serotype I (44 strains) were resistant to oxytetracycline and erythromycin and were found to possess both tet (S) and erm (B) genes. Minimum inhibitory concentrations (MICs) of oxytetracycline and erythromycin were 128 and 512 mg/mL, respectively. Southern blot hybridization analysis showed that the tet(S) gene was encoded on an 11-kb conjugative plasmid, while the erm(B) gene resided on the chromosomal DNA. All of the examined S. parauberis serotype II strains (20 strains) were tetracycline-resistant (MIC = 32 mg/mL) and were found to possess tet(M) gene. Both int (integrase) and xis (excisase) genes were also detected in all of the serotype II strains, suggesting the presence of Tn916-related element in these strains. These results present a threat of difficulty in treatment of S. parauberis infection by tetracyclines, especially those caused by serotype II strains.

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Section: Discussionmentioning

confidence: 99%

Characterization of Drug Resistance in Streptococcus parauberis Isolated from Japanese Flounder

2009

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“…The identification of multiple drug-resistant strains has indicated future problems in controlling the pathogen (Aoki et al 1990). Recently, oral and injectable vaccines against L. garvieae infection in Seriola quinqueradiata have been developed and commercialized in Japan.…”

Section: Introductionmentioning

confidence: 99%

Cell-surface properties of Lactococcus garvieae strains and their immunogenicity in the yellowtail Seriola quinqueradiata

Ooyama¹,

Hirokawa²,

Minami³

et al. 2002

Dis. Aquat. Org.

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The cell-surface properties of strains of Lactococcus garvieae were examined. Two capsular types were found, one with a highly developed capsule (KG9408) and one with a micro-capsule (MS93003) carrying fimbriae-like components projecting from the cell surface. One strain (NSS9310) had neither cell capsular nor fimbriae-like structures on its cell surface. The strains with the highly developed capsule were more virulent to fish than either the micro-capsular or non-capsular strains. The KG9408, MS93003 and NSS9310 strains could be clearly differentiated by their susceptibility to bacteriophages. Protection against L. garvieae infection was induced in the yellowtail Seriola quinqueradiata by immunization with formalin-killed L. garvieae KG9408 and MS93003 cells. Although protection was also induced by immunization with NSS9310, the level of protection was significantly lower than that with KG9408 and MS93003 vaccines. Passive immunization with yellowtail immune sera raised against KG9408 and MS93003 conferred strong protection on yellowtail with rapid bacterial clearance after challenge with L. garvieae. Immunoblotting analysis of protein antigens extracted from L. garvieae strains using rabbit anti-KG9408 and anti-MS93003 sera and yellowtail anti-KG9408 and anti-MS93003 sera indicated that some bands in KG9408 and MS93003 strains were not detectable in NSS9310.KEY WORDS: Immunogenicity ⋅ Lactococcus garvieae ⋅ Seriola quinqueradiata ⋅ Cell capsule ⋅ Fimbriae Resale or republication not permitted without written consent of the publisherDis Aquat Org 51: [169][170][171][172][173][174][175][176][177] 2002 thought to play roles in the pathogenicity of L. garvieae infection, possibly by increasing resistance to fish phagocytosis (Yoshida et al. 1996(Yoshida et al. , 1997.Control of Lactococcus garvieae infection in yellowtail culture has depended on chemotherapy with macrolides. The identification of multiple drug-resistant strains has indicated future problems in controlling the pathogen (Aoki et al. 1990). Recently, oral and injectable vaccines against L. garvieae infection in Seriola quinqueradiata have been developed and commercialized in Japan. Experimental vaccination against L. garvieae has been described (Iida et al. 1982, Sato et al. 1996 and has been reported to provide immunity and increased opsonic activity in the fish. However, no detailed information on the antigenicity of L. garvieae phenotypes nor the duration of the immunity was given. The protective mechanisms of the L. garvieae vaccine remains unknown.In a previous study, Ooyama et al. (1999) reported that formalin-killed Lactococcus garvieae KG-phenotype (capsulated phenotypes) cells and KG+ phenotype (noncapsulated) cells induced strong immunity in Seriola quinqueradiata against artificial infection and longlasting agglutinating titres against non-capsulated cells (avirulent KG+ phenotype). Furthermore, appendages (fimbriae-like structures) were seen extending from the cell surface of L. garvieae KG-phenotype, with some destruction of...

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“…The extensive use has led to an increase in drug resistant strains among fish pathogens. A transferable R plasmid has been suggested to be responsible for the drug resistance of several fish pathogenic bacteria including Aeromonas hydrophila (1), Aeromonas salmonicida (4), Edwardsiella tarda (7), Enterococcus seriolicida (9,18), and Vibrio anguillarum (5). Thus, the spread of R plasmids encoding multiple drug resistance has made chemotherapy of bacterial infections difficult in many fish farms (8).…”

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confidence: 99%

Drug Resistance and Broad Geographical Distribution of Identical R Plasmids of Pasteurella piscicida Isolated from Cultured Yellowtail in Japan

Kim

Aoki

1993

Microbiology and Immunology

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An MIC test of 12 chemotherapeutic agents performed on 175 strains of Pasteurella piscicida collected from cultured yellowtail (Seriola quinqueradiata) in different areas of Japan from 1989 to 1991 revealed 152 strains (87%) with resistance to combinations of ampicillin (AP), chloramphenicol (CP), kanamycin (KM), nalidixic acid (NA), sulfamonomethoxine (SA), tetracycline (TC), and/or trimethoprim (TMP). The remaining 23 strains were sensitive to all the drugs tested: AP, cefazolin, CP, florfenicol (FF), furazolidone, KM, NA, novobiocin, SA, streptomycin, TC, and TMP. FF showed the most effective antibacterial activity against P. piscicida with MICs ranging from 0.004 to 0.6 μg/ml. One hundred and forty‐nine of the 152 resistant strains carried transferable R plasmids encoding one of the Cp Km Sa Tc, Km Sa Tc, Km Sa, and Sa resistance. The most common resistance marker of transferable R plasmids identified in P. piscicida was Km Sa Tc. R plasmids encoding three different resistant markers were very similar on the basis of their digestion patterns with restriction endonucleases. There was homology among the DNAs of nine transferable R plasmids selected. Our findings suggest that multiple drug resistant strains of P. piscicida carrying transferable R plasmids with the same DNA structure are common in yellowtail farms and that the R plasmid has been retained within the P. piscicida population without change in their DNA structure according to geography and year.

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Drug resistance in a non-hemolytic Streptococcus sp. isolated from cultured yeltowtail Seriola quinqueradiata

Cited by 38 publications

References 5 publications

Characterization of Drug Resistance in Streptococcus parauberis Isolated from Japanese Flounder

Characterization of Drug Resistance in Streptococcus parauberis Isolated from Japanese Flounder

Cell-surface properties of Lactococcus garvieae strains and their immunogenicity in the yellowtail Seriola quinqueradiata

Drug Resistance and Broad Geographical Distribution of Identical R Plasmids of Pasteurella piscicida Isolated from Cultured Yellowtail in Japan

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