Characterization of Drug Resistance in Streptococcus parauberis Isolated from Japanese Flounder

Meng, Fei; Kanai, Kazuaki; Yoshikoshi, Kazuma

doi:10.3147/jsfp.44.40

Cited by 14 publications

(16 citation statements)

References 32 publications

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“…The results obtained in this study are consistent with those of previous reports indicating that ERY and OTC have limited efficacy toward S. parauberis isolated from olive flounder (Jeong et al, 2006;Park et al, 2009). Meng et al (2009) reported that S. parauberis strains with high-level resistance to OTC are often associated with resistance to ERY. This is likely because most erm genes encoding ERY resistance proteins are carried on transposons, and through transposition become linked to tet genes encoding tetracycline or OTC resistance proteins.…”

Section: Antibiotic Resistance Of Streptococcus Parauberis Strainssupporting

confidence: 93%

In vitro Antibacterial and Synergistic Activity of an Ecklonia cava Extract against Anti biotic-Resistant Streptococcus parauberis

Eom¹,

Santos²,

Kim³

et al. 2015

Fisheries and aquatic sciences

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In an effort to discover alternative phytotherapeutic antimicrobial agents to combat Streptococcus parauberis, a fish pathogenic bacterium, we evaluated the antibacterial activity of seaweed extracts in vitro. A methanolic extract of Ecklonia cava exhibited strong antibacterial activity against S. parauberis isolated from olive flounder Paralichthys olivaceus. Furthermore, the n-hexane soluble (Hexane) fraction of the E. cava methanolic extract exhibited the greatest antibacterial effect on S. parauberis strains with a minimum inhibitory concentration (MIC) ranging from 256 to 1,024 µg/mL. In addition, the MIC values of oxytetracycline against antibiotic-resistant S. parauberis were markedly reduced up to 64-fold in combination with the Hexane fraction, suggesting that the antibacterial activity of the antibiotic was restored when combined with the Hexane fraction. The interaction between both antibiotics and the Hexane fraction was assessed by the fractional inhibitory concentration (FIC) index. The Hexane fraction and oxytetracycline combination against antibiotic-resistant S. parauberis strains resulted in a median ∑FIC range of 0.502 to 0.516. Thus, the synergistic ranges of median ∑FIC < 1 were observed for all combinations of the Hexane fraction and oxytetracycline against S. parauberis. To the best of our knowledge, this is the first report indicating the efficacy of an E. cava extract against fish pathogenic bacterium S. parauberis.

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Section: Antibiotic Resistance Of Streptococcus Parauberis Strainssupporting

confidence: 93%

In vitro Antibacterial and Synergistic Activity of an Ecklonia cava Extract against Anti biotic-Resistant Streptococcus parauberis

Eom¹,

Santos²,

Kim³

et al. 2015

Fisheries and aquatic sciences

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“…According to our findings it is likely that the prevalence of the tet (M)‐positive serotype II strains in Japan are due to clonal dissemination of a original strain that acquired the Tn 916 ‐like element from another bacterial species rather than selection of tet (M)‐positive strains by frequent use of tetracyclines. This hypothesis is supported by the fact that all of the tested serotype II strains were also low resistant to erythromycin (Meng et al. 2009).…”

Section: Discussionmentioning

confidence: 79%

“…However, the worldwide use of tetracyclines for the control of infections, as prophylactic agents or as growth promoters, has facilitated the emergence and spread of acquired resistance (Roberts 2005). In our preliminary experiments (Meng et al. 2009), tetracycline‐resistant strains of S. parauberis serotype I and II were found in our culture collections, and, surprisingly, all of serotype II strains derived from divergent aquaculture sites were tetracycline‐resistant and harboured tet (M), xis (excisase) and int (integrase) genes which are components of Tn 916 family transposons (Clewell et al.…”

Section: Introductionmentioning

confidence: 90%

Structural characterization of Tn916-like element inStreptococcus parauberisserotype II strains isolated from diseased Japanese flounder

Meng

Kanai

Yoshikoshi

2009

Letters in Applied Microbiology

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Aims: To screen for the existence and determine the structure of Tn916‐like element in Streptococcus parauberis serotype II strains isolated from cultured Japanese flounder in western Japan. Methods and Results: In this study, the structure of Tn916‐like element and the flanking regions were characterized by polymerase chain reaction (PCR) and inverse PCR, followed by cloning and sequencing. The Tn916‐like element is 18 031 bp in length and composed of 22 ORFs. Southern blot hybridization analysis showed that the HincII‐digested internal structures of Tn916‐like elements yielded two patterns among S. parauberis serotype II strains. The flanking sequences were identical with the corresponding region of S. parauberis serotype I strain except for the addition of 6‐bp coupling sequence (ATCATA) being adjacent to the upstream of the element. Conclusion: The Tn916‐like element exhibited high homology (more than 99%) with Tn916 observed in other streptococci and enterococci and was integrated in the same site of chromosome for all of the tested S. parauberis serotype II strains. Significance and Impact of the Study: The results indicate that the Tn916‐like element encoding tet(M) gene is present in all of the tested S. parauberis serotype II strains, which are disseminated in the flounder‐culturing areas in western Japan.

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“…Strains of Streptococcus parauberis have previously shown resistance against different antimicrobial drugs such as tetracycline, oxytetracycline, and erythromycin [43,44]. Because of the chance of antibiotic resistance, there is a need for new and alternative therapeutic targets against this pathogenic bacterium.…”

Section: Discussionmentioning

confidence: 99%

Pharmacodynamics of Ceftiofur Selected by Genomic and Proteomic Approaches of Streptococcus parauberis Isolated from the Flounder, Paralichthys olivaceus

Boby

Abbas

Lee

et al. 2020

International Journal of Genomics

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We employed an integrative strategy to present subtractive and comparative metabolic and genomic-based findings of therapeutic targets against Streptococcus parauberis. For the first time, we not only identified potential targets based on genomic and proteomic database analyses but also recommend a new antimicrobial drug for the treatment of olive flounder (Paralichthys olivaceus) infected with S. parauberis. To do that, 102 total annotated metabolic pathways of this bacterial strain were extracted from computational comparative metabolic and genomic databases. Six druggable proteins were identified from these metabolic pathways from the DrugBank database with their respective genes as mtnN, penA, pbp2, murB, murA, coaA, and fni out of 112 essential nonhomologous proteins. Among these hits, 26 transmembrane proteins and 77 cytoplasmic proteins were extracted as potential vaccines and drug targets, respectively. From the FDA DrugBank, ceftiofur was selected to prevent antibiotic resistance as it inhibited our selected identified target. Florfenicol is used for treatment of S. parauberis infection in flounder and was chosen as a comparator drug. All tested strains of fish isolates with S. parauberis were susceptible to ceftiofur and florfenicol with minimum inhibitory concentrations (MIC) of 0.0039–1 μg/mL and 0.5–8 μg/mL, IC50 of 0.001–0.5 μg/mL and 0.7–2.7 μg/mL, and minimum biofilm eradication concentrations (MBEC) of 2–256 μg/mL and 4–64 μg/mL, respectively. Similar susceptibility profiles for ceftiofur and florfenicol were found, with ceftiofur observed as an effective and potent antimicrobial drug against both planktonic and biofilm-forming strains of the fish pathogen Streptococcus parauberis, and it can be applied in the aquaculture industry. Thus, our predictive approach not only showed novel therapeutic agents but also indicated that marketed drugs should also be tested for efficacy against newly identified targets of this important fish pathogen.

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Characterization of Drug Resistance in Streptococcus parauberis Isolated from Japanese Flounder

Cited by 14 publications

References 32 publications

In vitro Antibacterial and Synergistic Activity of an Ecklonia cava Extract against Anti biotic-Resistant Streptococcus parauberis

In vitro Antibacterial and Synergistic Activity of an Ecklonia cava Extract against Anti biotic-Resistant Streptococcus parauberis

Structural characterization of Tn916-like element inStreptococcus parauberisserotype II strains isolated from diseased Japanese flounder

Pharmacodynamics of Ceftiofur Selected by Genomic and Proteomic Approaches of Streptococcus parauberis Isolated from the Flounder, Paralichthys olivaceus

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