Drug release characteristics of PAMAM dendrimer–drug conjugates with different linkers

Kurtoglu, Yunus E.; Mishra, Manoj Kumar; Kannan, Sujatha; Kannan, Rangaramanujam M.

doi:10.1016/j.ijpharm.2009.10.017

Cited by 154 publications

(106 citation statements)

References 18 publications

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“…Freeze-drying was carried out to obtain the final product, which was characterized by UVVis, 1 H NMR, FTIR and HPLC. HPLC characterization of PAMAM-AB was carried out with HITACHI L-2000 series (Tokyo, Japan) HPLC Instrument equipped with a L-2130 pump, a L-2200 autosampler and a L-2400 UV-detector according to the method reported with a little modification (Kurtoglu et al, 2010). Data were collected by a D-2000 Elite HPLC chromatography workstation.…”

Section: Synthesis and Characterization Of Pamam-ab Conjugatesmentioning

confidence: 99%

“…UV absorbance peak area of AB was used to derive a calibration and used to determine the concentrations of free AB in samples. Another experiment was studied in pooled human plasma diluted to 80% with pH 7.4 PBS (Kurtoglu et al, 2010). The conjugate was dissolved in 2 mL pre-heated plasma solutions at a concentration of 0.5 mg/mL.…”

Section: Hydrolysis Stability Of Pamam-ab In Vitromentioning

confidence: 99%

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Synthesis of a novel polyamidoamine dendrimer conjugating with alkali blue as a lymphatic tracer and study on the lymphatic targeting in vivo

Yang

Xia

et al. 2014

Drug Delivery

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In this study, a novel lymphatic tracer polyamidoamin-alkali blue (PAMAM-AB) was synthesized in order to evaluate the intra-lymphatic targeting ability and lymphatic tropism of PAMAM-AB after subcutaneous administration. UV-Vis, FT-IR, NMR and HPLC characterization were performed to prove the successful synthesis of PAMAM-AB. The calculated AB payload of PAMAM-AB conjugate was seven per dendrimer molecule (27.16% by weight). Hydrolysis stability of PAMAM-AB in vitro was evaluated, which was stable in PBS and human plasma. Lymphatic tracing were studied to determine the blue-stained intensity of PAMAM-AB in right popliteral lymph nodes (PLNs), iliac lymph nodes (ILNs) and para-aortic lymph nodes (PALNs) after subcutaneous administration. The pharmacokinetics and biodistribution of PAMAM-AB in mice were investigated. PLNs, ILNs and PALNs could be obviously blue-stained within 10 min after PAMAM-AB administration, and displayed a more rapid lymphatic absorption, a higher AUC value in lymph nodes and a longer lymph nodes residence time compared with methylene blue solution (MB-S), MB water-in-oil microemulsion (MB-ME), MB multiple microemulsion (MB-MME). Enhanced lymphatic drainage from the injection site and uptake into lymph of PAMAM-AB indicated that PAMAM-AB possesses the double function of lymphatic tracing and lymphatic targeting, and suggested the potential for the development of lymphatic targeting vectors or as a lymphatic tracer in its own right.

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Section: Synthesis and Characterization Of Pamam-ab Conjugatesmentioning

confidence: 99%

Section: Hydrolysis Stability Of Pamam-ab In Vitromentioning

confidence: 99%

Synthesis of a novel polyamidoamine dendrimer conjugating with alkali blue as a lymphatic tracer and study on the lymphatic targeting in vivo

Yang

Xia

et al. 2014

Drug Delivery

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“…This dendrimer has ability to react with biological macromolecules such as DNA, proteins, peptides, antibodies, drugs and etc. 4,18,19 Nowadays, using immobilization of enzymes by various carriers like polymers has many applications in biological reactors and enzymatic sensors. Therefore, enzymatic immobilization methods are as an interesting field for researchers as a key stage in enzyme modification.…”

Section: -7mentioning

confidence: 99%

Covalent Immobilization of Trypsin on a Novel Aldehyde-Terminated PAMAM Dendrimer

Hamidi¹,

Rashidi²,

Asgari³

et al. 2012

Bulletin of the Korean Chemical Society

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Dendrimers are a novel class of nonlinear polymers and due to their extensive applications in different fields, called versatile polymers. Polyamidoamine (PAMAM) dendrimers are one of the most important dendrimers that have many applications in nanobiotechnology and industry. Generally aldehyde terminated dendrimers are prepared by activation of amine terminated dendrimers by glutaraldehyde which has two problems, toxicity and possibility of crosslink formation. In this study, novel aldehyde-terminated PAMAM dendrimer was prepared and used for covalent immobilization of trypsin by the aim of finding a special reagent which can prevent crosslinking and deactivation of the enzyme. For this purpose aminoacetaldehydedimethylacetal (AADA) was used as spacer group between aldehyde-terminated PAMAM and trypsin.The findings of this study showed that immobilization of trypsin not only resulted higher optimal temperature, but also increased the thermal stability of the immobilized enzyme in comparison to the free enzyme.

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“…[2][3][4][5][6][7][8][9][10] On the other hand, full-generation PAMAM dendrimers have surface amine groups (32 amine groups in the third-generation PAMAM dendrimer, G3), offering an easy route for formation of conjugates with drugs. [11][12][13][14][15][16][17][18][19][20][21] Due to the relatively low toxicity of PAMAM dendrimers, [22][23][24][25] they are frequently used as transdermal diffusion promoters. 26 In previous studies, we have used PAMAM dendrimers as carriers for 8-methoxypsoralen, the photosensitizer used in psoralen and ultraviolet light A therapy (PUVA), 4 riboflavin, 5 and vitamin C. 27 In all these cases, the PAMAM dendrimers formed host-guest complexes with 8-methoxypsoralen, vitamin B 2 and vitamin C, thereby modifying the transdermal flux of the guest molecule.…”

Section: Introductionmentioning

confidence: 99%

Bioconjugates of PAMAM dendrimers with trans-retinal, pyridoxal, and pyridoxal phosphate

Filipowicz¹,

Wołowiec²

2012

IJN

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Background: Bioconjugates of a polyamidoamine (PAMAM) G3 dendrimer and an aldehyde were synthesized as carriers for vitamins A and B 6 , and the bioavailability of these vitamins for skin nutrition was investigated. Methods: Nuclear magnetic resonance (NMR) and ultraviolet-visible methods were used to characterize the structure of the bioconjugates and for monitoring release of pyridoxal (Pyr) and pyridoxal phosphate (PLP) from these bioconjugates in vitro. A skin model permeation of bioconjugates was also studied in a Franz chamber. Results: A transdermal G3 PAMAM dendrimer was used to synthesize bioconjugates with trans-retinal (Ret), pyridoxal (Pyr), or PLP. These nanomolecules, containing up to four covalently linked Ret, Pyr, or PLP (G3 4Ret , G3 4Pyr , and G3 4PLP ), were able to permeate the skin, as demonstrated in vitro using a model skin membrane. PLP and Pyr bound to a macromolecular vehicle were active cofactors for glutamic pyruvic transaminase, as shown by 1 H NMR spectral monitoring of the progress of the L-alanine + α-ketoglutarate → glutamic acid + pyruvic acid reaction. Conclusion: PAMAM-PLP, PAMAM-Pyr, and PAMAM-Ret bioconjugates are able to permeate the skin. PLP and Pyr are available as cofactors for glutamic pyruvic transaminase.

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Drug release characteristics of PAMAM dendrimer–drug conjugates with different linkers

Cited by 154 publications

References 18 publications

Synthesis of a novel polyamidoamine dendrimer conjugating with alkali blue as a lymphatic tracer and study on the lymphatic targeting in vivo

Synthesis of a novel polyamidoamine dendrimer conjugating with alkali blue as a lymphatic tracer and study on the lymphatic targeting in vivo

Covalent Immobilization of Trypsin on a Novel Aldehyde-Terminated PAMAM Dendrimer

Bioconjugates of PAMAM dendrimers with trans-retinal, pyridoxal, and pyridoxal phosphate

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