Covalent Immobilization of Trypsin on a Novel Aldehyde-Terminated PAMAM Dendrimer

Abstract

Dendrimers are a novel class of nonlinear polymers and due to their extensive applications in different fields, called versatile polymers. Polyamidoamine (PAMAM) dendrimers are one of the most important dendrimers that have many applications in nanobiotechnology and industry. Generally aldehyde terminated dendrimers are prepared by activation of amine terminated dendrimers by glutaraldehyde which has two problems, toxicity and possibility of crosslink formation. In this study, novel aldehyde-terminated PAMAM dendrimer was prepared and used for covalent immobilization of trypsin by the aim of finding a special reagent which can prevent crosslinking and deactivation of the enzyme. For this purpose aminoacetaldehydedimethylacetal (AADA) was used as spacer group between aldehyde-terminated PAMAM and trypsin.The findings of this study showed that immobilization of trypsin not only resulted higher optimal temperature, but also increased the thermal stability of the immobilized enzyme in comparison to the free enzyme.

Self-templated chemically stable hollow spherical covalent organic framework

“…Immobilization of enzymes in mesoporous materials is an important research area in biomedical and pharmaceutical industries [45][46][47] . The immobilization process not only improves the recyclability of the costly enzyme, but most of the time it can improve the stability of the enzyme under extreme conditions 48 .…”

“…Since the COF-DhaTab is chemically stable and mesoporous (3.7 nm), we can study the adsorption and storage of the enzyme trypsin into the COF pores. Immobilization of enzyme into the mesoporous material is potentially useful for applications such as biosensors and biocatalysts because it can increase the recyclability of the costly enzyme and also improves the stability of enzymes under extreme conditions [45][46][47][48] .…”

Mitigation of bisphenol A using an array of laccase-based robust bio-catalytic cues – A review

“…Few reports have also documented the immobilization of enzymes on dendritic nanostructures, such as polyamidoamine-modified MNPs, and aldehyde-terminated polyamidoamine dendrimers for the mineralization of organic micro-pollutants (Hamidi et al, 2012;Daumann et al, 2014;Zhu et al, 2016). Hyper-branched polymer-biocatalyst bioconjugates are mounting as compelling support materials in the arena of materials science and biocatalysis (Ge et al, 2007).…”