Drug-Delivery System Based on Salmon DNA Nano- and Micro-Scale Structures

Abstract

Microneedles, fabricated by nano-moulding technology show great promise in the field of drug delivery by enabling the painless self-administration of drugs in a patient-friendly manner. In this study, double-stranded salmon DNA (SDNA) was used as both a drug-delivery vehicle and structural material with a microneedle system. SDNA is non-toxic and demonstrates good mechanical robustness, mouldability, biocompatibility, bio-absorbability, and binding affinity with drug molecules for bio-functional applications. Benign fabrication conditions to protect temperature-sensitive biomolecules are used to produce SDNA structures of various sizes with a high aspect ratio (4: 1). Unlike existing dissolving microneedle structure materials, the special binding characteristics of doxorubicin hydrochloride, anti-cancer drug molecules, and SDNA demonstrate the stability of drug-molecule encapsulation via UV-absorption and photoluminescence analyses. Based on COMSOL simulation and in vitro analysis of the stratum corneum of porcine skin, the mechanical functionality of SDNA microneedles was evaluated in vitro by penetrating the stratum corneum of porcine skin. The SDNA microneedle dissolved and drug permeation was assessed using rhodamine, a drug surrogate. Owing to its many beneficial characteristics, we anticipate that the SDNA microneedle platform will serve as an effective alternative for drug delivery.

Ultrasonically and Iontophoretically Enhanced Drug-Delivery System Based on Dissolving Microneedle Patches

“…PDMS (Sylgard 184; Dow Corning, Midland, MI, USA) prepared by mixing elastomer and curing agent in a ratio of 10: 1 was poured onto a master stamp. The molds were cured at 50 °C for 2 h. The PDMS mold was then separated from the stamp 10,11,42 .…”

“…It can effectively deliver drugs or active ingredients to the skin by crossing over the stratum corneum [5][6][7][8][9][10][11] . Among the various types of microneedles [5][6][7][8][9][10][11] , dissolving microneedles are made of a water-soluble polymer that exhibits excellent biocompatibility. The dissolving microneedles penetrate the skin and get dissolved by the fluid in the skin to deliver drugs or effective ingredients 8,10,11 .…”

“…Among the various types of microneedles [5][6][7][8][9][10][11] , dissolving microneedles are made of a water-soluble polymer that exhibits excellent biocompatibility. The dissolving microneedles penetrate the skin and get dissolved by the fluid in the skin to deliver drugs or effective ingredients 8,10,11 . On the other hand, the cavitation phenomenon is applied during ultrasound-mediated drug delivery.…”

Progress and perspective of microneedle system for anti-cancer drug delivery

“…The high loading capacity of the drugs and topical application provided strong support for in vivo therapeutic outcomes while minimizing the side effects [70]. interactions between DNA molecule and DOX, which made it a stable vehicle for drug delivery [80]. There are various materials used to develop MNs to explore release kinetics and skin diffusion of anticancer drugs such as dacarbazine [81], doxorubicin [73,78,82] and MTX [31,72].…”

Tailoring the growth and proliferation of human dermal fibroblasts by DNA‐based polymer films for skin regeneration

“…The biomedical use of DNA in regenerative medicine has also been prominent in recent years 2,4,10 . However, much of the effort focused mainly on the development of DNA nanostructures as drug delivery systems due to the lowest antigenicity of DNA as compared to other macromolecules and the ability of cytokines to be stacked between DNA bases 5,6,12‐14 . Our group's studies have demonstrated the high biocompatibility and excellent controlled drug release properties of DNA‐based polymeric adhesive films 7,9 .…”