Drosophila RecQ5 is involved in proper progression of early spermatogenesis

Sakurai, Haruna; Takai, Satoshi; Kawamura, Kasumi; Ogura, Yuji; Yoshioka, Yasuhide; Kawasaki, Katsumi

doi:10.1016/j.bbrc.2014.09.051

Cited by 2 publications

(2 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several DEAD box proteins have been associated with defects in sperm development. RecQ5 and Belle are both required for early spermatogenesis (Johnstone et al, 2005;Sakurai et al, 2014) and mutation of Rm62 has also been shown to cause male sterility (Buszczak et al, 2007). While the cause of sterility in Rm62 mutants has not been identified, mutation of Blanks, a binding partner for Rm62, results in spermiogenesis defects similar to those observed in Ddx1 null males (Gerbasi et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Loss of the Drosophila melanogaster DEAD box protein Ddx1 leads to reduced size and aberrant gametogenesis

Germain

Hildebrandt

et al. 2015

Developmental Biology

View full text Add to dashboard Cite

Mammalian DDX1 has been implicated in RNA trafficking, DNA double-strand break repair and RNA processing; however, little is known about its role during animal development. Here, we report phenotypes associated with a null Ddx1 (Ddx1(AX)) mutation generated in Drosophila melanogaster. Ddx1 null flies are viable but significantly smaller than control and Ddx1 heterozygous flies. Female Ddx1 null flies have reduced fertility with egg chambers undergoing autophagy, whereas males are sterile due to disrupted spermatogenesis. Comparative RNA sequencing of control and Ddx1 null third instars identified several transcripts affected by Ddx1 inactivation. One of these, Sirup mRNA, was previously shown to be overexpressed under starvation conditions and implicated in mitochondrial function. We demonstrate that Sirup is a direct binding target of Ddx1 and that Sirup mRNA is differentially spliced in the presence or absence of Ddx1. Combining Ddx1 null mutation with Sirup dsRNA-mediated knock-down causes epistatic lethality not observed in either single mutant. Our data suggest a role for Drosophila Ddx1 in stress-induced regulation of splicing.

show abstract

Section: Discussionmentioning

confidence: 99%

Loss of the Drosophila melanogaster DEAD box protein Ddx1 leads to reduced size and aberrant gametogenesis

Germain

Hildebrandt

et al. 2015

Developmental Biology

View full text Add to dashboard Cite

show abstract

“…Drosophila melanogaster is a powerful system for studying mechanisms of spermatogenesis, as it has powerful genetics, conserved genes and well‐characterized stages of spermatogenesis (Fabian & Brill, ; Fuller, ; Sakurai et al, ). Drosophila sperm development has many parallels to those in other organisms, including humans.…”

Section: Introductionmentioning

confidence: 99%

A new function of immunity‐related gene Zn72D in male fertility of Drosophila melanogaster

Biwot

Zhang

Chen

et al. 2019

Arch Insect Biochem Physiol

View full text Add to dashboard Cite

Zn72D encodes the Drosophila zinc finger protein Zn72D. It was first identified to be involved in phagocytosis and indicated to have a role in immunity. Then it was demonstrated to have a function in RNA splicing and dosage compensation in Drosophila melanogaster. In this study, we discovered a new function of Zn72D in male fertility. We showed that knockdown of Zn72D in fly testes caused an extremely low egg hatch rate. Immunofluorescence staining of Zn72D knockdown testes exhibited scattered spermatid nuclei and no actin cones or individualization complexes (ICs) during spermiogenesis, whereas the early-stage germ cells and the spermatocytes were observed clearly. There were no mature sperms in the seminal vesicles of Zn72D knockdown fly testes, although a few sperms could be found close to the seminal vesicle. We further showed that many cytoskeleton-related genes were significantly downregulated in fly testes due to Zn72D knockdown. Taken together these findings suggest that Zn72D may have an important function in spermatogenesis by sustaining the cytoskeletonbased morphogenesis and individualization thus ensuring the proper formation of sperm in D. melanogaster. K E Y W O R D S actin cone, Drosophila melanogaster, spermatid bundle, testis, Zn72D

show abstract

Drosophila RecQ5 is involved in proper progression of early spermatogenesis

Cited by 2 publications

References 28 publications

Loss of the Drosophila melanogaster DEAD box protein Ddx1 leads to reduced size and aberrant gametogenesis

Loss of the Drosophila melanogaster DEAD box protein Ddx1 leads to reduced size and aberrant gametogenesis

A new function of immunity‐related gene Zn72D in male fertility of Drosophila melanogaster

Contact Info

Product

Resources

About