Droplet optofluidic imaging for λ-bacteriophage detection via co-culture with host cell Escherichia coli

Yu, Jinqian; Huang, Weichen; Chin, Lip Ket; Lei, Liang; Lin, Zhi Ping; Ser, Wee; Chen, H.; Ayi, T. C.; Chen, C. H.; Liu, A. Q.

doi:10.1039/c4lc00042k

Cited by 40 publications

(28 citation statements)

References 25 publications

(24 reference statements)

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“…177 However, the need to create an aerosol prior to detectionmass spectrometry requires the input of sample ions in the gas phasewith these two methods precludes the use of a two-phase system wherein cells can be encapsulated preaerosol formation. 128 In a label-free analogue to the work from ref. 204 Direct optical detection is more readily integrated into microfluidic systems, given the transparent nature of the materials typically used (glass, PDMS, PMMA, etc.…”

Section: Single-cell Emulsions By Sortingmentioning

confidence: 99%

The Poisson distribution and beyond: methods for microfluidic droplet production and single cell encapsulation

et al. 2015

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There is a recognized and growing need for rapid and efficient cell assays, where the size of microfluidic devices lend themselves to the manipulation of cellular populations down to the single cell level. An exceptional way to analyze cells independently is to encapsulate them within aqueous droplets surrounded by an immiscible fluid, so that reagents and reaction products are contained within a controlled microenvironment. Most cell encapsulation work has focused on the development and use of passive methods, where droplets are produced continuously at high rates by pumping fluids from external pressure-driven reservoirs through defined microfluidic geometries. With limited exceptions, the number of cells encapsulated per droplet in these systems is dictated by Poisson statistics, reducing the proportion of droplets that contain the desired number of cells and thus the effective rate at which single cells can be encapsulated. Nevertheless, a number of recently developed actively-controlled droplet production methods present an alternative route to the production of droplets at similar rates and with the potential to improve the efficiency of single-cell encapsulation. In this critical review, we examine both passive and active methods for droplet production and explore how these can be used to deterministically and non-deterministically encapsulate cells.

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Section: Single-cell Emulsions By Sortingmentioning

confidence: 99%

The Poisson distribution and beyond: methods for microfluidic droplet production and single cell encapsulation

et al. 2015

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“…In optofluidic research, the advanced on-chip flow cytometry is usually divided into two optical categories: one is detected by the traditional far-field scattering light [45][46][47] and the other is detected by the near-field evanescent wave 48,49 . For example, Yu et al reported a cytometer to detect bacteriophage by the far-field scattering light and droplet optofluidic imaging (Fig.…”

Section: Biochemical Samples Manipulation and Sensing In Flowing Liquidsmentioning

confidence: 99%

“…For example, Yu et al reported a cytometer to detect bacteriophage by the far-field scattering light and droplet optofluidic imaging (Fig. 5(b)) 47 . The sample alignment on both vertical and horizontal direction was realized by the flexible 3D hydrodynamic.…”

Section: Biochemical Samples Manipulation and Sensing In Flowing Liquidsmentioning

confidence: 99%

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Optofluidics: the interaction between light and flowing liquids in integrated devices

Zhu¹,

Zhu²,

Zuo³

et al. 2019

Opto-Electronic Advances

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Optofluidics is a rising technology that combines microfluidics and optics. Its goal is to manipulate light and flowing liquids on the micro/nanoscale and exploiting their interaction in optofluidic chips. The fluid flow in the on-chip devices is reconfigurable, non-uniform and usually transports substances being analyzed, offering a new idea in the accurate manipulation of lights and biochemical samples. In this paper, we summarized the light modulation in heterogeneous media by unique fluid dynamic properties such as molecular diffusion, heat conduction, centrifugation effect, light-matter interaction and others. By understanding the novel phenomena due to the interaction of light and flowing liquids, quantities of tunable and reconfigurable optofluidic devices such as waveguides, lenses, and lasers are introduced. Those novel applications bring us firm conviction that optofluidics would provide better solutions to high-efficient and high-quality lab-on-chip systems in terms of biochemical analysis and environment monitoring.

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“…Various microfluidic devices have been introduced for the study of cell cultivation and high-throughput screening (Shen et al 2014); (Falconnet et al 2011). Diverse methods of droplet generation and droplet capture have been developed to monitor cell growth (Jakiela et al 2013;Yu et al 2014). In this study, a low-cost oil and surfactant combination that was stable under high temperature (50 °C) was developed.…”

Section: Introductionmentioning

confidence: 99%

Quantitative analysis of the growth of individual Bacillus coagulans cells by microdroplet technology

Zhu

Shi

Chu

et al. 2018

Bioresour. Bioprocess.

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Background: Cellular physiological responses, which are often obscured by inferences from population-level data, are of great importance in cell biology. Microfluidics has emerged as an important tool for biological research on a small scale, reaching even the single-cell level. Results:In this work, a flow-focusing microdroplet generator was developed to produce monodisperse microdroplets with high stability. Individual B. coagulans cells were encapsulated in the microdroplets and cultured offline. The specific growth rate of B. coagulans at the single-cell level was analyzed, and the growth of B. coagulans in the droplets showed good consistency with that in flasks, with a correlation coefficient of 0.98. The morphological heterogeneity and its potential relationship with the production of lactic acid by B. coagulans were evaluated using a microscopic imaging method. Conclusion:We have demonstrated a single-cell monitoring methodology based on microdroplets. This approach has great potential for studying a range of behavioral and physiological features of bacteria at the single-cell level. which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Droplet optofluidic imaging for λ-bacteriophage detection via co-culture with host cell Escherichia coli

Cited by 40 publications

References 25 publications

The Poisson distribution and beyond: methods for microfluidic droplet production and single cell encapsulation

The Poisson distribution and beyond: methods for microfluidic droplet production and single cell encapsulation

Optofluidics: the interaction between light and flowing liquids in integrated devices

Quantitative analysis of the growth of individual Bacillus coagulans cells by microdroplet technology

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