Droplet electric separator microfluidic device for cell sorting

Guo, Feng; Ji, Xinghu; Liu, Kan; He, Rongxiang; Zhao, Libo; Guo, Zhi-Xiao; Liu, Wei; Guo, Shishang; Zhao, Xingzhong

doi:10.1063/1.3360812

Cited by 91 publications

(65 citation statements)

References 18 publications

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“…Another recent study carried out by Guo et al [59] has demonstrated sorting of water droplets in oil using electrophoresis. It was reported that this method allows to achieve considerably higher throughput in continuous flow.…”

Section: Electric Forcementioning

confidence: 99%

Sorting and manipulation of biological cells and the prospects for using optical forces

Atajanov

Zhbanov

Yang

2018

Micro and Nano Syst Lett

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Contemporary biomedical research requires development of novel techniques for sorting and manipulation of cells within the framework of a microfluidic chip. The desired functions of a microfluidic chip are achieved by combining and integrating passive methods that utilize the channel geometry and structure, as well as active methods that include magnetic, electrical, acoustic and optical forces. Application of magnetic, electric and acoustics-based methods for sorting and manipulation have been and are under continuous scrutiny. Optics-based methods, in contrast, have not been explored to the same extent as other methods, since they attracted insufficient attention. This is due to the complicated, expensive and bulky setup required for carrying out such studies. However, advances in optical beam shaping and computer hardware, and software have opened up new opportunities for application of light to development of advanced sorting and manipulation techniques. This review outlines contemporary techniques for cell sorting and manipulation, and provides an in-depth view into the existing and prospective uses of light for cell sorting and manipulation.

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Section: Electric Forcementioning

confidence: 99%

Sorting and manipulation of biological cells and the prospects for using optical forces

Atajanov

Zhbanov

Yang

2018

Micro and Nano Syst Lett

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“…The amazingly fast development of lab-on-a-chip technology in recent decades has had a profound impact on the food and healthcare industries [1]- [3], as well as novel applications in bio-defense against bioterrorism and bio-warfare [4]. Many experimental techniques for microparticle manipulation have been extensively reported in prior studies, such as using biochemical [5], electrokinetic [6], optical [7], and magnetic [8] methods. Compared to these conventional techniques, another popular method that utilizes acoustics to drive microfluidic actuation has shown distinct advantage as an easy tool for manipulation of colloidal particles [9]- [11].…”

Section: Rf-activated Standing Surface Acoustic Wavementioning

confidence: 99%

“…Substituting (5) into (1), the acoustic radiation force on a rigid sphere can then be obtained, (6) For a very small compressible spherical particle , the time-averaged acoustic radiation force has been derived by Eller [25], (7) where is the instantaneous particle volume. For a small sphere, can be written as [25] …”

Section: A Dynamics Of Particle Aggregation Under Ssawmentioning

confidence: 99%

RF-Activated Standing Surface Acoustic Wave for On-Chip Particle Manipulation

Guo

Chen

et al. 2014

IEEE Trans. Microwave Theory Techn.

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Abstract-On-chip flow cytometry provides a powerful tool to characterize cell samples for point-of-care diagnosis. In particular, sample focusing at specific locations along the microchannel is crucial to ensure the accuracy of detection. In this paper, we present a simple strategy of interfacing an RF-activated standing surface acoustic wave (SSAW) substrate with a microfluidic channel, and use this device to study the dynamic process of particle aggregation along the microchannel. Specifically, the SSAW generated by two parallel interdigital transducers induces an acoustic radiation force that propels particles suspended in the liquid toward the pressure nodes whose locations are tunable by judicious choice of the applied SSAW frequency. We also carry out a theoretical analysis that provides an estimation of the time for the particle assembly, which is validated by experimental results. This SSAW transducer can therefore be easily integrated into a microfluidic chip with moderate energy consumption, offering a convenient and effective solution in the development of on-chip flow cytometry.

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“…As an important branch of microfluidics, droplet-based microfluidics own innate advantages in biochemical assays. The droplet is a separate, micro-sized reaction chamber, which makes it possible to minimize the energy consumption and the required reagents, and to better control the mixture/reaction parameters [19][20][21][22]. While, to our best knowledge, there is little work on utilizing droplet-based microfluidic systems for Hg(II) ion detection.…”

Section: Introductionmentioning

confidence: 99%

A Concentration-Controllable Microfluidic Droplet Mixer for Mercury Ion Detection

et al. 2015

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Abstract:A microfluidic droplet mixer is developed for rapid detection of Hg(II) ions. Reagent concentration and droplets can be precisely controlled by adjusting the flow rates of different fluid phases. By selecting suitable flow rates of the oil phase, probe phase and sample phase, probe droplets and sample droplets can be matched and merged in pairs and subsequently well-mixed in the poly (dimethylsiloxane) (PDMS) channels. The fluorescence enhancement probe (Rhodamine B mixed with gold nanoparticles) encapsulated in droplets can react with Hg(II) ions. The Hg(II) ion concentration in the sample droplets is adjusted from about 0 to 1000 nM through fluid regulation to simulate possible various contaminative water samples. The intensity of the emission fluorescence is sensitive to Hg(II) ions (increases as the Hg(II) ion concentration increases). Through the analysis of the acquired fluorescence images, the concentration of Hg(II) ions can be precisely detected. With the advantages of less time, cost consumption and easier manipulations, this device would have a great potential in micro-scale sample assays and real-time chemical reaction studies.

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Droplet electric separator microfluidic device for cell sorting

Cited by 91 publications

References 18 publications

Sorting and manipulation of biological cells and the prospects for using optical forces

Sorting and manipulation of biological cells and the prospects for using optical forces

RF-Activated Standing Surface Acoustic Wave for On-Chip Particle Manipulation

A Concentration-Controllable Microfluidic Droplet Mixer for Mercury Ion Detection

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