Sorting and manipulation of biological cells and the prospects for using optical forces

Atajanov, Arslan; Zhbanov, A. I.; Yang, Sung

doi:10.1186/s40486-018-0064-3

Cited by 47 publications

(34 citation statements)

References 138 publications

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“…The optical field was used to power the propulsion of the Janus micromotor. [154] Living organisms can be assembled using optical forces [27,[155][156][157] Optical tweezers generate microvortices that serve as optical traps. For example, different kinds of cells including yeast (nonmotile) and Chlamydomonas reinhardtii (motile), were assembled into micrometer-sized dynamic cellular arrays with welldefined spatiotemporal positioning (Figure 7a).…”

Section: Optical Reversible Dynamic Assemblymentioning

confidence: 99%

“…The use of external fields triggers different processes that lead to the formation of assemblies. [24] They can i) create pressure nodes or tweezers to concentrate and trap micro-objects in a specific location, [25,26] ii) modulate particle-particle interactions that can lead to attractive or repulsive behavior between the subunits that compose the assembly [27,28] or iii) use active matter (micromotors or living organisms) as a seed unit to form assemblies with larger particles, or induce swarming behavior. [29][30][31][32][33][34] We thoroughly discuss each of the external fields, their mechanisms of action, and the latest applications in the synthetic and biological assembly in the following sections.…”

Section: Introductionmentioning

confidence: 99%

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Reversible Design of Dynamic Assemblies at Small Scales

Soto

Wang

Deshmukh

et al. 2020

Advanced Intelligent Systems

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Emerging bottom-up fabrication methods have enabled the assembly of synthetic colloids, microrobots, living cells, and organoids to create intricate structures with unique properties that transcend their individual components. Herein, an access point to the latest developments is provided in externally driven assembly of synthetic and biological components. In particular, reversibility is emphasized, which enables the fabrication of multiscale systems that would not be possible under traditional techniques. Magnetic, acoustic, optical, and electric fields are the most promising methods for controlling the reversible assembly of biological and synthetic subunits as they can reprogram their assembly by switching on/off the external field or shaping these fields. Capabilities are featured to dynamically actuate the assembly configuration by modulating the properties of the external stimuli, including frequency and amplitude. The design principles are designed, which enable the assembly of reconfigurable structures. Finally, the high degree of control capabilities offered by externally driven assembly will enable broad access to increasingly robust design principles toward building advanced dynamic intelligent systems is foreseen.

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Section: Optical Reversible Dynamic Assemblymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Reversible Design of Dynamic Assemblies at Small Scales

Soto

Wang

Deshmukh

et al. 2020

Advanced Intelligent Systems

View full text Add to dashboard Cite

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“…This makes holographic tweezers highly suitable to investigate the hydrodynamic behaviour of colloidal systems [15][16][17] and to control optically trapped particles for studying statistical physics phenomena and ensembles out of equilibrium [18,19]. HOTs also provide new insights into the operation of micromachines [20,21] and represent a powerful manipulation tool in optofluidic lab-on-a-chip devices [13], for sorting and sensing applications on particles, droplet and biomolecules [22][23][24]. Another significant success granted by optical tweezers is represented by its unprecedented access to the nanoscopic world.…”

Section: Introductionmentioning

confidence: 99%

Optical tweezers in single-molecule experiments

et al. 2020

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In the last decades, optical tweezers have progressively emerged as a unique tool to investigate the biophysical world, allowing to manipulate and control forces and movements of one molecule at a time with unprecedented resolution. In this review, we present the use of optical tweezers to perform single-molecule force spectroscopy investigations from an experimental perspective. After a comparison with other single-molecule force spectroscopy techniques, we illustrate at an introductory level the physical principles underlying optical trapping and the main experimental configurations employed nowadays in single-molecule experiments. We conclude with a brief summary of some remarkable results achieved with this approach in different biological systems, with the aim to highlight the great variety of experimental possibilities offered by optical tweezers to scientists interested in this research field.

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“…[ 22 ] However, HOT setup is bulky, complex, and expensive. [ 23 ] The 6D manipulation implemented in HOT system may be difficult to be transferred to other OT system, such as the easy‐to‐setup planar OT system based on time multiplexing techniques; more general methods for out‐of‐plane manipulation should be explored.…”

Section: Introductionmentioning

confidence: 99%

Distributed Force Control for Microrobot Manipulation via Planar Multi‐Spot Optical Tweezer

Zhang

Barbot

et al. 2020

Advanced Optical Materials

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manipulation of cells, [3,4] microrobots, trapped in the focal position of the laser beam can be utilized as end-effectors of the OT. [5] Therefore, developing optical micro-machines to enable dexterous indirect manipulation of OT can have a wide range of applications. For example, four streptavidin-functionalized polystyrene beads, controlled by a time-shared multiplexed OT, have been attached to a biotinylated red blood cell and served as micro-tools to analyze the underlying molecular mechanisms involved in cell flickering. [6] In life science, laser-driven spinning birefringent spheres have been used to generate microfluidic-induced shear force for control of the growth of individual axons (nerve fibers) precisely, paving the way for nerve repair and regeneration. [7] To enable more dexterous indirect optical trapping and optical manipulation, optical micro robotics with novel synthetic structures can be used to increase the flexibility of OT systems and facilitate the deployment of general robotic solutions for biomedical science. [8] With recent advances in two-photon polymerization and other emerging micro/nanofabrication techniques, steerable non-spherical particles can now be fabricated. [9] Therefore, optically driven microrobots with complex structures can be used for arbitrary translational and angular positioning in 3D space. For example, anisotropic shapes have been considered for the design of micro-particles to create a remotely driven micro-motor based on radiation pressure for micromechanical systems. [10] An indirect optical trapping method using light driven micro-rotors to create a new form of near-field hydrodynamic micro-manipulation has been proposed, [11] with which the 2D trapping of absorbing particles, the control of position and orientation of yeast cells, and the independent control over multiple objects simultaneously have been demonstrated. Apart from using OT to realize reconfigurable hydrodynamic manipulation via the control of optically driven micro-motors, optoelectronic tweezer has been developed to combine dielectrophoresis with OT, which is capable of exerting a stronger manipulation force for a given intensity of light for microrobot control. [12] A shaped particle based on a tapered cylinder fabricated by two-photon polymerization has been proposed for acting as a passive force clamp, [13] which facilitates the design of optically trapped objects with specific force profiles. However, the motions of the passive force clamp are mainly for planar translation. For micro-manipulation which demands Optical tweezers (OT) represent a versatile tool for micro-manipulation. To avoid damages to living cells caused by illuminating laser directly on them, microrobots controlled by OT can be used for manipulation of cells or living organisms in microscopic scale. Translation and planar rotation motion of microrobots can be realized by using a multi-spot planar OT. However, out-of-plane manipulation of microrobots is difficult to achieve with a planar OT. This paper presents a distribut...

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Sorting and manipulation of biological cells and the prospects for using optical forces

Cited by 47 publications

References 138 publications

Reversible Design of Dynamic Assemblies at Small Scales

Reversible Design of Dynamic Assemblies at Small Scales

Optical tweezers in single-molecule experiments

Distributed Force Control for Microrobot Manipulation via Planar Multi‐Spot Optical Tweezer

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