Advanced Driver Assistance Systems (ADAS) proved a potential to save lives and reduce serious injury in traffic accidents. But if they are not known and accepted by the drivers, they will not deliver the benefits intended by their designers. The aim of the current research was to assess the awareness and acceptance of selected advanced driver assistance systems among a sample of Czech drivers, as well as the factors that might influence it. Together, 526 drivers participated in the questionnaire study. About a half of them was not aware of any further information besides the existence of various ADAS and more than 70 % did not drive with such systems yet. As for the acceptance, most of the systems were desired by at least 50 % of the respondents, but the majority was not willing to pay any extra money for the systems. A perceived safety benefit might increase the acceptance, but in the end, a thorough driver education about the systems' function and limitations is needed.