Dried blood spot testing for the antenatal screening of HTLV, HIV, syphilis, toxoplasmosis and hepatitis B and C: prevalence, accuracy and operational aspects

Boa‐Sorte, Ney; Purificação, Antônio; Amorim, Tatiana; Assunção, Lorena; Reis, Alan; Galvão–Castro, Bernardo

doi:10.1016/j.bjid.2014.05.009

Cited by 24 publications

(20 citation statements)

References 38 publications

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“…Such advantages have been demonstrated by the experience and results of neonatal screening programs over the last 40 years for a large group of pathologies. 6 In the present study, it was verified that the frequencies of the infections, detected on paper filter, in pregnant women in the two studied regions in general were compatible with those found in other regions of the country, except for syphilis and the presence of sickle cell trait which presented a higher percentage. In addition, due to the implementation phase of the Programa da Rede Cegonha (Stork Network Program) and considering the fact that the data represents its initial phase, demanding expansion and consolidation in order to achieve better results, coverage rates in the South and Southwest macro-regions in Bahia were considered satisfactory.…”

Section: Variablessupporting

confidence: 86%

“…The use of paper filter for screening on pregnant women has shown to be quite effective, both in economic, as biosecurity and reliability aspects. 6 In this study, it was sought to estimate the rate of early detection of pathology screening, through the use of paper filter, as well as the coverage on adhesion and scope on the Programa de Triagem Pré-natal em Papel de Filtro da Rede Cegonha (Prenatal Screening Program on Paper Filter from the Stork Network), in the South and Southwest macro-regions in the State of Bahia. In addition, there was a concern to trace these pregnant women's epidemiological profile on the screening program.…”

Section: Discussionmentioning

confidence: 99%

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Screening on prenatal infections in the South and Southwest macro-regions in the State of Bahia, Brazil: detected on paper filter

Pedro¹,

Silva

Rebouças

et al. 2019

Rev. Bras. Saude Mater. Infant.

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Objectives: to estimate the detection rate on prenatal screening pathologies on paper filter in the South and Southwest in the State of Bahia, as well as to delineate the epidemiological profile of these pregnant women, calculate and estimate the rate of adherence and the coverage of the Program. Methods: a descriptive study was carried out from August 2013 to August 2015, and the data were obtained from the Labimuno/ICS/UFBA. Results: 64,743 pregnant women were included; the mean ages were 25 years for the Southwest macro-region and 23 for the South. The results on the screening tests showed positivity of 0.13% and 0.29% for HBsAg, 0.17% and 0.22% for cytomegalovirus, 0.07% and 0.09% for HCV, 0.13% and 0.38% for HTLV, 0.04% and 0.19% for HIV, 1.2% and 2.84% for syphilis, and 0.54% and 0.73% for toxoplasmosis in the Southwest and South macro-regions, respectively. The estimates on coverage were considered satisfactory. Sickle cell anemia showed positivity of 0.02% and of 0.04% and 5% and 6.3% presented sickle cell trait in the Southwest and South macro-regions, respectively. Conclusions: the frequencies of infections in maternal-fetal health were considered low, highlighting on syphilis and the presence for sickle cell trait.

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Section: Variablessupporting

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Screening on prenatal infections in the South and Southwest macro-regions in the State of Bahia, Brazil: detected on paper filter

Pedro¹,

Silva

Rebouças

et al. 2019

Rev. Bras. Saude Mater. Infant.

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“…A large prospective study of pregnant women in Europe, also used DBS samples from France, England and Germany [ 35 ], and it is worthwhile to note that of the 104 samples repeatedly reactive by particle agglutination test or ELISA, only 83 could be confirmed by Western blot or Line Immuno-Assay. Furthermore, a recent study comparing 692 DBS and their corresponding venous blood samples for the serological detection of several infections, found that both methods successfully detected several cases, including one HTLV infection [ 29 ], although no confirmation assay was used. Finally, one large study analyzed 55,293 DBS for HTLV seroprevalence, and although Western blot was not used for confirmation, it was possible to investigate the corresponding blood samples for confirmation [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

“…DBS were shown to be reliable for detecting anti-HIV-1/HIV-2 antibodies and serological markers of other pathogens, using either in-house or commercial assays to analyze the eluates [ 27 , 28 ]. The use of DBS in the detection of anti-HTLV antibodies has also been validated in several studies [ 29 – 31 ]. One early study initially established the methodology using simulated DBS, (i.e.…”

Section: Introductionmentioning

confidence: 99%

No significant HTLV seroprevalence in German people who inject drugs

et al. 2017

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BackgroundAlthough human T-lymphotropic virus (HTLV) is transmitted via the same routes as human immunodeficiency virus (HIV), its worldwide seroprevalence differs drastically because HTLV is transmitted mainly via infected cells rather than free virus. The sharing of needles and other equipment places people who inject drugs (PWID) at particularly high-risk for such blood-borne diseases.MethodsTo validate the methodology used to process and analyze the dried blood spots (DBS) utilized in the study, dried serum spots (DSS) with dilutions of sera from known HTLV infected individuals were analyzed by ELISA and Western blot. DBS collected between 2011 and 2015 from 2,077 PWID in eight German cities recruited by respondent-driven sampling were tested for HTLV-specific antibodies.ResultsThe validation demonstrated that the use of DSS allowed identification of samples with even low titers of HTLV-specific antibodies, although a confirmatory Western blot with an additional venous blood sample would often be required. Despite numerous HIV and HCV positive individuals being identified within the study population, none tested positive for HTLV.ConclusionWhile the HIV and HCV prevalences in German PWID are comparable to those in other European countries, the very low prevalence of HTLV reflects the situation in the general population.

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“…In the case of syphilis, EIA is used to detect treponemal antibodies of both classes (IgG and IgM). These tests have good sensitivity and specificity and have been used in some settings in Brazil as routine antenatal screening, including the investigation of other infections in the same blood sample [ 40 - 43 ].…”

Section: Reviewmentioning

confidence: 99%

Current Perspectives on Prevention of Mother-to-Child Transmission of Syphilis

Lago¹

2016

Cureus

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This article aims to provide an update on the prevention of mother-to-child transmission of syphilis by drawing upon some important basic concepts and reviewing the most recent literature on the diagnosis and treatment of syphilis in pregnancy. New technologies, such as automated and point-of-care immunologic tests, are shifting some paradigms, which will certainly be further investigated in the forthcoming years. This is the time to carefully evaluate traditional as well as new strategies to prevent congenital syphilis. Adverse outcomes of mother-to-child transmission of syphilis can be prevented with antenatal screening and penicillin therapy, which proved to have an excellent cost-benefit ratio even in populations with a low prevalence of syphilis. However, syphilis epidemiology is influenced by socioeconomic and cultural factors, and major challenges are faced by poor and developing countries in which the severity of the problem is extremely alarming. On the other hand, the emergence of new technologies has raised doubts about the best algorithm to be used when proper laboratory resources are available. Conditions are quite heterogeneous across populations, and some procedures should not be generalized while there is no evidence that supports some changes and while in-depth studies about local conditions are not conducted. Official organizations need to be alert in order to avoid isolated decisions and ensure that evidence-based guidelines be used in the management of syphilis in pregnancy.

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Dried blood spot testing for the antenatal screening of HTLV, HIV, syphilis, toxoplasmosis and hepatitis B and C: prevalence, accuracy and operational aspects

Abstract: The use of dried blood spot may represent a secure way to expedite access to results of vertically transmitted diseases in the prenatal period, particularly in regions with scarce healthcare resources.

Cited by 24 publications

References 38 publications

Screening on prenatal infections in the South and Southwest macro-regions in the State of Bahia, Brazil: detected on paper filter

Screening on prenatal infections in the South and Southwest macro-regions in the State of Bahia, Brazil: detected on paper filter

No significant HTLV seroprevalence in German people who inject drugs

Current Perspectives on Prevention of Mother-to-Child Transmission of Syphilis

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