Dressings and topical agents containing hyaluronic acid for chronic wound healing

Roehrs, Hellen; Stocco, Janislei Gd; Pott, Franciele Soares; Blanc, Gisely; Crozeta, Karla; Meier, Marineli Joaquim; Dias, Fernando Al

doi:10.1002/14651858.cd012215

Cited by 11 publications

(8 citation statements)

References 39 publications

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“…However, in the aforementioned studies, the influence of the addition of HMWHY onto the material-associated inflammatory tissue reaction was not sufficiently analyzed. It can be assumed that the addition of HY has an influence on the inflammatory alignment of the tissue reaction to a biomaterial, to support the material-related healing processes as has already been shown in other applications [32,33,34]. In general, it is assumed that the application of a biomaterial that enables a stronger anti-inflammatory tissue reaction may also lead to improved bone tissue regeneration [35].…”

Section: Discussionmentioning

confidence: 99%

The Addition of High Doses of Hyaluronic Acid to a Biphasic Bone Substitute Decreases the Proinflammatory Tissue Response

Sieger

Koržinskas

Jung

et al. 2019

IJMS

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Biphasic bone substitutes (BBS) are currently well-established biomaterials. Through their constant development, even natural components like hyaluronic acid (HY) have been added to improve both their handling and also their regenerative properties. However, little knowledge exists regarding the consequences of the addition of HY to their biocompatibility and the inflammatory tissue reactions. Thus, the present study was conducted, aiming to analyze the influence of two different amounts of high molecular weight HY (HMWHY), combined with a BBS, on in vitro biocompatibility and in vivo tissue reaction. Established in vitro procedures, using L929 cells, were used for cytocompatibility analyses under the test conditions of DIN EN:ISO 10993-5. For the in vivo part of the study, calvarial defects were created in 20 Wistar rats and subsequently filled with BBS, and BBS combined with two different HMWHY amounts, i.e., BBS + HY(L) and BBS + HY(H). As controls, empty defects were used. Established histological, immunohistochemical, and histomorphometrical methods were applied to analyze the tissue reactions to the three different materials, including the induction of pro- and anti-inflammatory macrophages and multinucleated giant cells (BMGCs). The in vitro results showed that none of the materials or compositions caused biological damage to the L929 cells and can be considered to be non-toxic. The in vivo results showed that only the addition of high doses of HY to a biphasic bone substitute significantly decreases the occurrence of pro-inflammatory macrophages (* p < 0.05), comparable to the numbers found in the control group, while no significant differences within the three study groups for M2-macrophages nor BMGCs were detected. In conclusion, the addition of different amounts of HMWHY does not seem to affect the inflammation response to BBS, while improving the material handling properties.

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Section: Discussionmentioning

confidence: 99%

The Addition of High Doses of Hyaluronic Acid to a Biphasic Bone Substitute Decreases the Proinflammatory Tissue Response

Sieger

Koržinskas

Jung

et al. 2019

IJMS

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“…Chronic wounds develop because of a defective regulation of processes involved in wound healing. They can be the result of severe injuries (e.g., burns, trauma, surgery) as well as main or side effects of chronic diseases (e.g., diabetes, venous disease, neurological conditions) . Chronic wounds have a strong impact on patients as they experience pain, reduced mobility and work ability, and often social isolation.…”

Section: Introductionmentioning

confidence: 99%

“…A variety of wound dressing strategies can be employed to cope with chronic wounds, aiming to maintain a moist environment with materials like films, foams, and hydrogels that contain water‐adsorbing materials (e.g., hyaluronan, alginate), to reduce infections (e.g., dressings containing silver ions, chitosan, or disinfectants) and to support healing and structural reconstruction (e.g., collagens, glycosaminoglycans, growth factors) . Nevertheless, the majority of wound dressings today tend to focus only on some of the aforementioned issues without addressing all aspects in a concerted manner.…”

Section: Introductionmentioning

confidence: 99%

Effect of Different Crosslinking Strategies on Physical Properties and Biocompatibility of Freestanding Multilayer Films Made of Alginate and Chitosan

Apte

Repanas

Willems

et al. 2019

Macromolecular Bioscience

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Freestanding multilayer films prepared by layer‐by‐layer technique have attracted interest as promising materials for wound dressings. The goal is to fabricate freestanding films using chitosan (CHI) and alginate (ALG) including subsequent crosslinking to improve the mechanical properties of films while maintaining their biocompatibility. Three crosslinking strategies are investigated, namely use of calcium ions for crosslinking ALG, 1‐ethyl‐3‐(‐3‐dimethylaminopropyl) carbodiimide combined with N‐hydroxysuccinimide for crosslinking ALG with CHI, and Genipin for crosslinking chitosan inside the films. Different characteristics, such as surface morphology, wettability, swelling, roughness, and mechanical properties are investigated showing that films became thinner, exhibited rougher surfaces, had lower water uptake, and increased mechanical strength after crosslinking. Changes of wettability are moderate and dependent on the crosslinking method. In vitro cytotoxicity and cell attachment studies with human dermal fibroblasts show that freestanding CHI‐ALG films represent a poorly adhesive substratum for fibroblasts, while studies using incubation of plastic‐adherent fibroblast beneath floating films show no signs of cytotoxicity in a time frame of 7 days. Results from cell experiments combined with film characteristics after crosslinking, indicate that crosslinked freestanding films made of ALG and CHI may be interesting candidates for wound dressings.

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“…Moisture retention of dECM grafts is an important design aspect for soft tissue reconstruction as the ability for a graft to maintain a moist wound environment promotes cell migration, reduces scarring and fibrosis, and improves angiogenesis. 76 In vitro testing herein was intended to reflect a ‘worst case’ scenario, with no moisture added to the test sample after initial hydration. As expected, the addition of the HA layer to the CMP samples improved the device moisture retention properties, approximately doubling the time to complete moisture loss of the device ( Figure 6 ).…”

Section: Discussionmentioning

confidence: 99%

Further structural characterization of ovine forestomach matrix and multi-layered extracellular matrix composites for soft tissue repair

Smith¹,

Dempsey²,

Veale³

et al. 2021

J Biomater Appl

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Decellularized extracellular matrix (dECM)–based biomaterials are of great clinical utility in soft tissue repair applications due to their regenerative properties. Multi-layered dECM devices have been developed for clinical indications where additional thickness and biomechanical performance are required. However, traditional approaches to the fabrication of multi-layered dECM devices introduce additional laminating materials or chemical modifications of the dECM that may impair the biological functionality of the material. Using an established dECM biomaterial, ovine forestomach matrix, a novel method for the fabrication of multi-layered dECM constructs has been developed, where layers are bonded via a physical interlocking process without the need for additional bonding materials or detrimental chemical modification of the dECM. The versatility of the interlocking process has been demonstrated by incorporating a layer of hyaluronic acid to create a composite material with additional biological functionality. Interlocked composite devices including hyaluronic acid showed improved in vitro bioactivity and moisture retention properties.

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Dressings and topical agents containing hyaluronic acid for chronic wound healing

Cited by 11 publications

References 39 publications

The Addition of High Doses of Hyaluronic Acid to a Biphasic Bone Substitute Decreases the Proinflammatory Tissue Response

The Addition of High Doses of Hyaluronic Acid to a Biphasic Bone Substitute Decreases the Proinflammatory Tissue Response

Effect of Different Crosslinking Strategies on Physical Properties and Biocompatibility of Freestanding Multilayer Films Made of Alginate and Chitosan

Further structural characterization of ovine forestomach matrix and multi-layered extracellular matrix composites for soft tissue repair

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