Draft Plastid and Mitochondrial Genome Sequences from Antarctic Alga
            <i>Prasiola crispa</i>

Carvalho, Evelise Leis; Wallau, Gabriel Luz; Rangel, Darlene Lopes; Machado, Laís Ceschini; Silva, Alexandre Freitas da; Silva, Luiz Fernando Duarte da; Macedo, Pablo Echeverria; Pereira, Antônio Batista; Victoria, Filipe de Carvalho; Boldo, Juliano Tomazzoni; Belo, Cháriston André Dal; Pinto, Paulo Marcos

doi:10.1128/genomea.01151-15

Cited by 6 publications

(4 citation statements)

References 10 publications

(8 reference statements)

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“…This wide range of properties makes living marine organisms a promising source of molecules to develop drugs to treat diseases or pathologies. Prasiola crispa is a terrestrial green alga (Chlorophyta, Trebouxiophyceae) of the Antarctic continent, with interesting biological features, grows on moist soils fertilized by penguin guano, resists repeated thawing cycles and freezing, and absorbs high levels of UV radiation during summer (Carvalho et al 2015;Jacob et al 1991Jacob et al , 1992. It is well known that organisms that live in polar regions are subjected to extreme and harsher abiotic and atmosphere conditions (low temperatures and humidity, frequent and rapid fluctuations from freezing to thawing, severe winds, and long periods of light and darkness), and as consequence, they develop natural strategies to face such aggressive conditions, allowing them to survive under these most unfavorable environment.…”

Section: Introductionmentioning

confidence: 99%

The seaweed Prasiola crispa (Chlorophyta) neutralizes toxic effects of Bothrops jararacussu snake venom

et al. 2016

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In Brazil, the snake genus Bothrops has many venomous species with 90 % of cases of accidents. Snake bites by Bothrops jararacussu result in moderate to severe envenoming, characterized by hemorrhage, coagulation disorders, tissue necrosis, and death. Antivenom has been regularly used for more than a century but poorly neutralizes myonecrosis. And, as a consequence, victims may have their affected limbs amputated. Thus, the production of antivenom must be improved as well as alternative treatments investigated. Thus, the ability of four extracts of the green alga Prasiola crispa to neutralize some toxic effects of B. jararacussu venom was tested. P. crispa was collected in Antarctica, then extracted using four solvents, dichloromethane (DCM), ethyl acetate (ETA), n-hexane (HEX), or methanol (MET). The extracts were incubated with B. jararacussu venom, and in vivo (hemorrhagic, lethal, and edematogenic) or in vitro (coagulating and proteolytic) activities were performed. Moreover, B. jararacussu venom was injected into mice before or after the injection of alga extracts. Overall, extracts inhibited all activities. The MET extract inhibited less and HEX, DCM and ETA inhibited more efficiently the activities. These latter extracts fully protected mice from B. jararacussu-induced hemorrhage and delayed death of mice. Edema was partially inhibited (20 %) by all extracts. Neutralization of hemorrhage was also observed when the extracts of P. crispa were administered after or before the venom injection. These results indicate that the extracts of P. crispa have potential to treat or to prevent some toxic effects of B. jararacussu venom, thus aiding in the antivenom therapy.

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Section: Introductionmentioning

confidence: 99%

The seaweed Prasiola crispa (Chlorophyta) neutralizes toxic effects of Bothrops jararacussu snake venom

et al. 2016

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“…Karlusich et al 2015 ). However, up to now, only the draft plastid and mitochondrial genome sequences from P. crispa have been published (Carvalho et al 2015 ); therefore, no direct evidence on the occurrence of these protection mechanisms in Prasiola is available.…”

Section: Discussionmentioning

confidence: 99%

The terrestrial green macroalga Prasiola calophylla (Trebouxiophyceae, Chlorophyta): ecophysiological performance under water-limiting conditions

et al. 2017

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The phylogenetic placement of Prasiola calophylla, from an anthropogenic habitat previously shown to contain a novel UV sunscreen compound, was confirmed by analysis of its rbcL gene. This alga has the capacity to tolerate strong water-limiting conditions. The photosynthetic performance and ultrastructural changes under desiccation and osmotic stress were investigated. Freshly harvested thalli showed an effective quantum yield of PSII [Y(II)] of 0.52 ± 0.06 that decreased to ∼60% of the initial value at 3000 mM sorbitol, and 4000 mM sorbitol led to a complete loss of Y(II). The Y(II) of thalli exposed to controlled desiccating conditions at 60% relative humidity (RH) ceased within 240 min, whereas zero values were reached after 120 min at 20% RH. All investigated samples completely recovered Y(II) within ∼100 min after rehydration. Relative electron transport rates (rETR) were temperature dependent, increasing from 5, 10, to 25 °C but strongly declining at 45 °C. Transmission electron microscopy of samples desiccated for 2.5 h showed an electron dense appearance of the entire cytoplasm when compared to control samples. Thylakoid membranes were still visible in desiccated cells, corroborating the ability to recover. Control and desiccated cells contained numerous storage lipids and starch grains, providing reserves. Overall, P. calophylla showed a high capacity to cope with water-limiting conditions on a physiological and structural basis. A lipophilic outer layer of the cell walls might contribute to reduce water evaporation in this poikilohydric organism.

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“…P. crispa represents the first organism of the Prasiolales order with an available transcriptome since, until this work, the mitochondrial and plastid genomes were the only molecular data available for this species (Carvalho et al, 2015 , 2017 ). Therefore, the purpose of this study was to sequence the transcriptome of P. crispa .…”

Section: Introductionmentioning

confidence: 99%

De novo Assembly and Annotation of the Antarctic Alga Prasiola crispa Transcriptome

Carvalho

Maciel

Macedo

et al. 2018

Front. Mol. Biosci.

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Draft Plastid and Mitochondrial Genome Sequences from Antarctic Alga Prasiola crispa

Cited by 6 publications

References 10 publications

The seaweed Prasiola crispa (Chlorophyta) neutralizes toxic effects of Bothrops jararacussu snake venom

The seaweed Prasiola crispa (Chlorophyta) neutralizes toxic effects of Bothrops jararacussu snake venom

The terrestrial green macroalga Prasiola calophylla (Trebouxiophyceae, Chlorophyta): ecophysiological performance under water-limiting conditions

De novo Assembly and Annotation of the Antarctic Alga Prasiola crispa Transcriptome

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