De novo Assembly and Annotation of the Antarctic Alga Prasiola crispa Transcriptome

Carvalho, Evelise Leis; Maciel, Lucas F.; Macedo, Pablo Echeverria; Dezordi, Filipe Zimmer; Abreu, Maria E. T.; Victoria, Filipe de Carvalho; Pereira, Antônio Batista; Boldo, Juliano Tomazzoni; Wallau, Gabriel da Luz; Pinto, Paulo Marcos

doi:10.3389/fmolb.2017.00089

Cited by 7 publications

(6 citation statements)

References 28 publications

(29 reference statements)

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“…Antarctica is a continent with extreme environmental conditions for life to thrive and this limitation leads to survival adaptation mechanisms in microorganisms 29 . One of the extreme environmental conditions in the Antarctic environment is the high incidence of UV which is a natural selective factor, and which favors the appearance of microorganisms resistant to solar radiation.…”

Section: Resultsmentioning

confidence: 99%

Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01

Reis-Mansur

Cardoso-Rurr

Silva

et al. 2019

Sci Rep

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The Microbacterium sp. LEMMJ01 isolated from Antarctic soil does not belong to any of the nearest species identified in the RDP database. Under UV radiation (A, B and C wavebands) the survival fractions of Microbacterium sp. cells were much higher compared with wild-type E . coli K12A15. Especially remarkable for an Antarctic bacterium, an expressive resistance against high UV-B doses was observed. The increased survival of DNA repair-proficient E . coli grown overnight added of 0.1 mg/ml or 1 mg/ml of the whole pigment extract produced by Microbacterium sp. revealed that part of the resistance of Microbacterium sp. against UV-B radiation seems to be connected with photoprotection by its pigments. Scanning electron microscopy revealed that UV-A and UV-B ensued membrane alterations only in E . coli . The APCI-MS fingerprints revealed the diagnostic ions for neurosporene (m/z 580, 566, 522, 538, and 524) synergism for the first time in this bacterium by HPLC-MS/MS analysis. Carotenoids also were devoid of phototoxicity and cytotoxicity effects in mouse cells and in human keratinocytes and fibroblasts.

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Section: Resultsmentioning

confidence: 99%

Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01

Reis-Mansur

Cardoso-Rurr

Silva

et al. 2019

Sci Rep

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“…Também é possível prospectar genes de interesse, como proteínas de resistência ao frio para possível inserção em culturas agrícolas [36,37], bem como rastrear organismos de acordo com sua origem. Este último exemplo inclui estudos recentes sobre rastreabilidade genética de drogas ilícitas [38,39] e também da identificação e rastreio das novas variantes do SARS-CoV-2, o coronavírus responsável pela pandemia global de COVID-19 [40,41].…”

Section: Contribuições Do Ngsunclassified

Sequenciamento NGS: Status e Perspectivas

Freitas¹,

Pinto²

2021

BIOINFO - Revista Brasileira De Bioinformática E Biologia Computacional

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Generation Sequencing (NGS), ou Sequenciamento de nova Geração são o conjunto de técnicas de Biologia Molecular e Bioinformática utilizadas para obter a sequência de ácidos nucleicos de uma amostra biológica. A utilização dessas técnicas vêm crescendo vertiginosamente desde a sua criação, quinze anos atrás, e se expande por várias áreas da ciência como saúde humana, agricultura e conservação da biodiversidade. Neste artigo mostramos as principais plataformas de NGS utilizadas atualmente e discutimos as principais contribuições da técnica, bem como as perspectivas do NGS para o futuro. Introdução O Sequenciamento de Nova Geração (NGS -sigla em inglês para Next Generation Sequencing ou na tradução "Sequenciamento de Próxima Geração") é o nome dado ao conjunto das mais modernas técnicas para a identificação das sequências de ácidos nucleicos (DNA e RNA) das mais variadas amostras biológicas, tais como plantas, animais e microrganismos [1]. São técnicas que se sobrepuseram ao modelo de Sanger [2] após o Projeto Genoma Humano e que surgiram da necessidade de se produzir sequenciamentos mais rápidos, econômicos e de qualidade [3]. O primeiro sequenciador de nova geração, o 454, lançado em 2005 (veja abaixo mais informações), já era capaz de sequenciar com uma capacidade 50x maior que o principal sequenciador de Sanger disponível na época e causou um certo alvoroço na comunidade científica [3,4]. Num primeiro momento as técnicas foram criticadas principalmente por produzirem sequências pequenas, entre 50 e 300 pares de bases, enquanto o método de Sanger produzia fragmentos na casa dos 750 pb [3], mas o tempo mostrou que a técnica tinha muito potencial a entregar. Já nos primeiros trabalhos se sobressaíram características como o grande número de sequências produzidas, a quantidade de reações que podiam ser paralelizadas e a

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“…The peptide sequence data were deposited in the UniProt Knowledgebase under the accession number C0HLU5. We searched for the most probable cDNA of Pc-frLHC by using the determined internal peptide sequences from the total mRNA libraries of P. crispa (BioProject ID: PRJNA329112) 39 . The cDNA sequence of Pc-frLHC gene was submitted to Third PArty data (TPA) of the DDBJ/EMBL/GenBank databases and was assigned the accession numbers TPA: BR001753.…”

Section: Amino Acid Sequence Analysismentioning

confidence: 99%

Uphill energy transfer mechanism for photosynthesis in the Antarctic alga

Kosugi¹,

Kawasaki

Shibata

et al. 2021

Preprint

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Prasiola crispa, a major green alga in Antarctica, forms layered colonies for survival under the severe terrestrial conditions of Antarctica, which include severe cold, drought, and strong sunlight. As a result of these conditions, the surface cells of P. crispa and other Antarctic organisms face high risk of photodamage. Cells of deeper layer escape from photodamage at the sacrifice of photosynthetic active radiation except infrared. P. crispa achieves effective photosynthesis by low energy far-red light for photosystem II excitation with high efficiency similar to that of visible light. Here, we identified a far-red light-harvesting complex of photosystem II in P. crispa, Pc-frLHC, and proposed a molecular mechanism of uphill excitation energy transfer based on its cryogenic electron-microscopy structure. While Pc-frLHC is associated with photosystem II, it is evolutionarily related to the light-harvesting complex of photosystem I. Pc-frLHC forms a ring-shaped homo-undecamer in which all chlorophyll a molecules are energetically connected and contains chlorophyll a trimers. It seems that the trimers are long-wavelength-absorbing chlorophylls for far-red light at 708 nm, and further absorbance extension is accomplished by Davydov-splitting in dimeric chlorophylls. The chlorophyll network should enable a highly efficient entropy-driven uphill excitation energy transfer using far-red light up to 725 nm.

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De novo Assembly and Annotation of the Antarctic Alga Prasiola crispa Transcriptome

Cited by 7 publications

References 28 publications

Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01

Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01

Sequenciamento NGS: Status e Perspectivas

Uphill energy transfer mechanism for photosynthesis in the Antarctic alga

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