Draft Genome Sequences of Two Marinitoga camini Isolates Producing Bacterioviruses

Mercier, Coraline; Lossouarn, Julien; Haverkamp, Thomas; Bienvenu, Nadège; Godfroy, Anne; Cueff-Gauchard, Valérie; Geslin, Claire; Nesbø, Camilla

doi:10.1128/genomea.01261-16

Cited by 3 publications

(4 citation statements)

References 10 publications

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“…Here, we report on the functional and genomic analysis of two new bacterioviruses that infect bacteria from the Marinitoga genus. Marinitoga camini virus 1 (MCV1) and Marinitoga camini virus 2 (MCV2) are siphoviruses that infect Marinitoga camini DV1197 and Marinitoga camini DV1155 respectively (Mercier et al, 2016). These bacteria are thermophiles and were isolated from deep-sea hydrothermal vents in the Mid-Atlantic Ridge.…”

Section: Introductionmentioning

confidence: 99%

Two viruses, MCV1 and MCV2, which infect Marinitoga bacteria isolated from deep‐sea hydrothermal vents: functional and genomic analysis

Mercier

Lossouarn

Nesbø

et al. 2017

Environmental Microbiology

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Viruses represent a driving force in the evolution of microorganisms including those thriving in extreme environments. However, our knowledge of the viral diversity associated to microorganisms inhabiting the deep-sea hydrothermal vents remains limited. The phylum of Thermotogae, including thermophilic bacteria, is well represented in this environment. Only one virus was described in this phylum, MPV1 carried by Marinitoga piezophila. In this study, we report on the functional and genomic characterization of two new bacterioviruses that infect bacteria from the Marinitoga genus. Marinitoga camini virus 1 and 2 (MCV1 and MCV2) are temperate siphoviruses with a linear dsDNA genome of 53.4 kb and 50.5 kb respectively. Here, we present a comparative genomic analysis of the MCV1 and MCV2 viral genomes with that of MPV1. The results indicate that even if the host strains come from geographically distant sites, their genomes share numerous similarities. Interestingly, heavy metals did not induce viral production, instead the host of MCV1 produced membrane vesicles. This study highlights interaction of mobile genetic elements (MGE) with their hosts and the importance of including hosts-MGEs' relationships in ecological studies.

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Section: Introductionmentioning

confidence: 99%

Two viruses, MCV1 and MCV2, which infect Marinitoga bacteria isolated from deep‐sea hydrothermal vents: functional and genomic analysis

Mercier

Lossouarn

Nesbø

et al. 2017

Environmental Microbiology

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“…values between strain BP5-C20A T genome and other related Marinitoga species for which genome data were available: M. lauensis LG1 T [44], M. camini str. 1197 [45], M. camini str.…”

Section: Conflict Of Interest Statementmentioning

confidence: 99%

“…After extraction using a modified version of the method of Miller Table 1. Digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between the strain BP5-C20A T genome and other related Marinitoga species for which genome data were available: M. lauensis LG1 T [46], M. litoralis DSM 21709 T , M. hydrogenitolerans DSM 16785 T , M. camini strain 1197 [47], M. camini strain 1155 [47], Marinitoga sp. 1137, M. piezophila KA3 T [48].…”

Section: Fatty Acid Analysesmentioning

confidence: 99%

Marinitoga aeolica sp. nov., a novel thermophilic anaerobic heterotroph isolated from a shallow hydrothermal field of Panarea Island in the Aeolian archipelago, Italy

Postec,

Galès,

Prime

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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A novel thermophilic strain, designated BP5-C20AT, was isolated from the shallow hydrothermal field of the Panarea island in the Aeolian archipelago close to Sicily, Italy. Cells are motile rods surrounded with a ‘toga’, Gram-stain-negative and display a straight to curved morphology during the exponential phase. Strain BP5-C20AT is thermophilic (optimum 55 °C), moderately acidophilic (optimum pH 5.6) and halotolerant (optimum 25 g l−1 NaCl). It can use yeast extract, peptone and tryptone. It uses the following carbohydrates: cellobiose, fructose, glucose, maltose, starch, sucrose and xylan. Elemental sulphur is used as an electron acceptor and reduced to hydrogen sulphide. The predominant cellular fatty acid is C16 : 0. Phylogenetic analysis showed that strain BP5-C20AT shared 97.3 % 16S rRNA gene sequence identity with the closest related species Marinitoga lauensis LG1T. The complete genome of strain BP5-C20AT is 2.44 Mb in size with a G+C content of 27.3 mol%. The dDDH and ANI values between the genomes of strains BP5-C20AT and M. lauensis LG1T are 31.0 and 85.70% respectively. Finally, from its physiological, metabolic and genomic characteristics, strain BP5-C20AT (=DSM 112332T=JCM 39183 T) is proposed as representative of a novel species of the genus Marinitoga named Marinitoga aeolica sp. nov. and belonging to the order Petrotogales , in the phylum Thermotogota .

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“…1137. All five proviral sequences encode for a LexA type transcriptional repressor, which has been suggested to function in viral cycle control[19]. The five sequences also contain multiple transcriptional regulators with XRE domains, and they share several hypothetical protein genes with no putative function assigned.It was demonstrated that the MPV1 virions of M. piezophila not only carry the viral DNA but preferentially package a plasmid of 13.3 kb (pMP1) also carried in M. piezophila.…”

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confidence: 99%

Marinitoga lauensis sp. nov., a novel deep-sea hydrothermal vent thermophilic anaerobic heterotroph with a prophage

L’Haridon

Gouhier

John

et al. 2019

Systematic and Applied Microbiology

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Draft Genome Sequences of Two Marinitoga camini Isolates Producing Bacterioviruses

Abstract: Here, we present the draft genome sequences of two thermophilic Marinitoga strain members of the Thermotogales order, Marinitoga camini DV1155 and Marinitoga camini DV1197. These strains were isolated from deep-sea hydrothermal vents of the Mid-Atlantic Ridge.

Cited by 3 publications

References 10 publications

Two viruses, MCV1 and MCV2, which infect Marinitoga bacteria isolated from deep‐sea hydrothermal vents: functional and genomic analysis

Two viruses, MCV1 and MCV2, which infect Marinitoga bacteria isolated from deep‐sea hydrothermal vents: functional and genomic analysis

Marinitoga aeolica sp. nov., a novel thermophilic anaerobic heterotroph isolated from a shallow hydrothermal field of Panarea Island in the Aeolian archipelago, Italy

Marinitoga lauensis sp. nov., a novel deep-sea hydrothermal vent thermophilic anaerobic heterotroph with a prophage

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