Marinitoga lauensis sp. nov., a novel deep-sea hydrothermal vent thermophilic anaerobic heterotroph with a prophage

L’Haridon, Stéphane; Gouhier, Léna; John, Emily St.; Reysenbach, Anna‐Louise

doi:10.1016/j.syapm.2019.02.006

Cited by 19 publications

(17 citation statements)

References 37 publications

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“…Differential characteristics between strain BP5-C20A T and other members of the genus Marinitoga Strains: 1, BP5-C20A T (this study); 2, M. lauensis LG1 T[8]; 3, M. okinawensis TFS-10-5 T[11]; 4, M. piezophila KA3 T[12]; 5, M. camini MV1075 T[10]; 6, M. litoralis MC3 T[6] ; 7, M. hydrogenitolerans AT1271 T[9]; 8, M. artica 2PyrY55-1 T[7]. +, Positive; (+), weakly positive; −, negative; nd, not determined.…”

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confidence: 71%

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Marinitoga aeolica sp. nov., a novel thermophilic anaerobic heterotroph isolated from a shallow hydrothermal field of Panarea Island in the Aeolian archipelago, Italy

Postec,

Galès,

Prime

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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A novel thermophilic strain, designated BP5-C20AT, was isolated from the shallow hydrothermal field of the Panarea island in the Aeolian archipelago close to Sicily, Italy. Cells are motile rods surrounded with a ‘toga’, Gram-stain-negative and display a straight to curved morphology during the exponential phase. Strain BP5-C20AT is thermophilic (optimum 55 °C), moderately acidophilic (optimum pH 5.6) and halotolerant (optimum 25 g l−1 NaCl). It can use yeast extract, peptone and tryptone. It uses the following carbohydrates: cellobiose, fructose, glucose, maltose, starch, sucrose and xylan. Elemental sulphur is used as an electron acceptor and reduced to hydrogen sulphide. The predominant cellular fatty acid is C16 : 0. Phylogenetic analysis showed that strain BP5-C20AT shared 97.3 % 16S rRNA gene sequence identity with the closest related species Marinitoga lauensis LG1T. The complete genome of strain BP5-C20AT is 2.44 Mb in size with a G+C content of 27.3 mol%. The dDDH and ANI values between the genomes of strains BP5-C20AT and M. lauensis LG1T are 31.0 and 85.70% respectively. Finally, from its physiological, metabolic and genomic characteristics, strain BP5-C20AT (=DSM 112332T=JCM 39183 T) is proposed as representative of a novel species of the genus Marinitoga named Marinitoga aeolica sp. nov. and belonging to the order Petrotogales , in the phylum Thermotogota .

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confidence: 71%

“…Tolerance to oxygen was examined and strain BP5-C20A T was a strict anaerobe as M. lauensis [8] and M. okinawensis [11].…”

Section: Physiological Testsmentioning

confidence: 99%

Marinitoga aeolica sp. nov., a novel thermophilic anaerobic heterotroph isolated from a shallow hydrothermal field of Panarea Island in the Aeolian archipelago, Italy

Postec,

Galès,

Prime

et al. 2023

International Journal of Systematic and Evolutionary Microbiology

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“…One of the 20 proviruses is present with 100% nucleotide sequence identity in all six available Thermosipho melanesiensis genomes (Haverkamp et al ., 2018), and therefore is counted as just one novel provirus. An additional provirus was reported in a Marinitoga lauensis genome after we completed the screening (L'Haridon et al ., 2019). Due to its similarity to proviruses identified in other Marinitoga genomes, it was not included in our further analyses.…”

Section: Resultsmentioning

confidence: 99%

Newly identified proviruses in Thermotogota suggest that viruses are the vehicles on the highways of interphylum gene sharing

Haverkamp

Lossouarn

Zhaxybayeva

et al. 2021

Environmental Microbiology

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Phylogenomic analyses of bacteria from the phylum Thermotogota have shown extensive lateral gene transfer (LGT) with distantly related organisms, particularly with Firmicutes.One likely mechanism of such DNA transfer is viruses. However, to date only three temperate viruses have been characterized in this phylum, all infecting bacteria from the Marinitoga genus. Here we report 17 proviruses integrated into genomes of eight Thermotogota genera and induce viral particle production from one of the proviruses. The proviruses fall into two groups based on sequence similarity, gene synteny and taxonomic classification. Proviruses of one group are found in six genera and are similar to the previously identified Marinitoga viruses, while proviruses from the second group are only distantly related to the proviruses of the first group, have different genome organization and are found in only two genera. Both groups are closely related to Firmicutes in genomic and phylogenetic analyses, and one of the groups show evidence of very recent LGT and are therefore likely capable of infecting cells from both phyla.We conjecture that viruses are responsible for a large portion of the observed gene flow between Firmicutes and Thermotogota.

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“…One of the proviruses is present with 100% nucleotide sequence identity in all six available Thermosipho melanesiensis genomes [41], and therefore is counted as just one novel provirus. An additional provirus (MLaV1) was reported in a Marinitoga lauensis genome after we completed the screening [42]. Due to its similarity to proviruses identified in other Marinitoga genomes, it was not included in our further analyses.…”

Section: Resultsmentioning

confidence: 99%

“…SC_TOLDC (AYSI01). We also included in this figure, MLaV1 - Marinitoga lauensis (QHLX01) reported in reference [53] after our bioinformatic screening was performed. The regions with significant pairwise tblastx similarity scores are connected (minimum identity: 30 %, minimum lenght : 100 nucleotides), red indicates that sequence is in the same direction while blue indicates that the similar sequences are on opposite strands.…”

Section: Resultsmentioning

confidence: 99%

Newly identified proviruses in Thermotogota suggest that viruses are the vehicles on the highways of interphylum gene sharing

Haverkamp

Lossouarn

Zhaxybayeva

et al. 2020

Preprint

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Viruses are drivers of microbial ecology and evolution controlling populations and disseminating DNA laterally among the cells they infect. Phylogenetic and comparative genomic analyses of Thermotogota bacteria have shown high levels of lateral gene transfer with distantly related organisms, particularly with Firmicutes. One likely source of such DNA transfers is viruses, however, to date only three temperate viruses infecting Marinitoga bacteria have been characterized in this phylum. Here we use a bioinformatic approach to identify an additional 17 proviruses integrated into genomes of eight Thermotogota genera including Marinitoga. We also induce viral particle production from one of the newly identified proviruses. The proviruses fall into two groups based on sequence similarities, gene synteny and virus classification tools. Group-1 shows similar genome structure to the three previously identified Marinitoga viruses while Group-2 are distantly related to these viruses and have different genome organization. Both groups show close connections to Firmicutes in genomic- and phylogenetic analyses, and the Group-2 viruses show evidence of very recent transfer between these lineages and are likely capable of infecting cells from both phyla. We suggest that viruses are responsible for a large portion of the laterally transferred DNA between these distantly related lineages.

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Marinitoga lauensis sp. nov., a novel deep-sea hydrothermal vent thermophilic anaerobic heterotroph with a prophage

Abstract: The EMBL 16S rRNA gene sequence accession number of strain LG1 T is MH457605. The genome sequence has been deposited in GenBank under the accession number QHLX00000000.

Cited by 19 publications

References 37 publications

Marinitoga aeolica sp. nov., a novel thermophilic anaerobic heterotroph isolated from a shallow hydrothermal field of Panarea Island in the Aeolian archipelago, Italy

Marinitoga aeolica sp. nov., a novel thermophilic anaerobic heterotroph isolated from a shallow hydrothermal field of Panarea Island in the Aeolian archipelago, Italy

Newly identified proviruses in Thermotogota suggest that viruses are the vehicles on the highways of interphylum gene sharing

Newly identified proviruses in Thermotogota suggest that viruses are the vehicles on the highways of interphylum gene sharing

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