Draft Genome Sequences of Two Ulvan-Degrading Isolates, Strains LTR and LOR, That Belong to the
            <i>Alteromonas</i>
            Genus

Kopel, Moran; Helbert, William; Henrissat, Bernard; Doniger, Tirza; Banin, Ehud

doi:10.1128/genomea.01081-14

Cited by 11 publications

(9 citation statements)

References 12 publications

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“…LTR, and Pseudoalteromonas sp. PLSV, (8,12,13). Genome annotation was manually confirmed by CAZy 4 and revealed that these strains encoded many polysaccharide-degrading enzymes.…”

Section: Table 2 Primers Used In This Studymentioning

confidence: 99%

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New Family of Ulvan Lyases Identified in Three Isolates from the Alteromonadales Order

Kopel

Helbert

Belnik

et al. 2016

Journal of Biological Chemistry

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Ulvan is the main polysaccharide component of the Ulvales (green seaweed) cell wall. It is composed of disaccharide building blocks comprising 3-sulfated rhamnose linked to D-glucuronic acid (GlcUA), L-iduronic acid (IdoUA), or D-xylose (Xyl). The degradation of ulvan requires ulvan lyase, which catalyzes the endolytic cleavage of the glycoside bond between 3-sulfated rhamnose and uronic acid according to a ␤-elimination mechanism. The first characterized ulvan lyase was identified in Nonlabens ulvanivorans, an ulvanolytic bacterial isolate. In the current study, we have identified and biochemically characterized novel ulvan lyases from three Alteromonadales isolated bacteria. Two homologous ulvan lyases (long and short) were found in each of the bacterial genomes. The protein sequences have no homology to the previously reported ulvan lyases and therefore are the first representatives of a new family of polysaccharide lyases. The enzymes were heterologously expressed in Escherichia coli to determine their mode of action. The heterologous expressed enzymes were secreted into the milieu subsequent to their signal sequence cleavage. An endolytic mode of action was observed and studied using gel permeation chromatography and 1 H NMR. In contrast to N. ulvanivorans ulvan lyase, cleavage occurred specifically at the GlcUA residues. In light of the genomic context and modular structure of the ulvan lyase families identified to date, we propose that two ulvan degradation pathways evolved independently.

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“…LTR, and Pseudoalteromonas sp. PLSV, (8,12,13). Genome annotation was manually confirmed by CAZy 4 and revealed that these strains encoded many polysaccharide-degrading enzymes.…”

Section: Table 2 Primers Used In This Studymentioning

confidence: 99%

“…LTR, and Pseudoalteromonas sp. PLSV (12,13). Inspection of the genomes did not reveal genes encoding proteins homologous to N. ulvanivorans ulvan lyase.…”

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confidence: 96%

New Family of Ulvan Lyases Identified in Three Isolates from the Alteromonadales Order

Kopel

Helbert

Belnik

et al. 2016

Journal of Biological Chemistry

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“…This draft genome, along with the additional draft sequences of N. ulvanivorans ( 10 ) and two Alteromonas spp. ( 14 ), will allow a better understanding of the utilization and exploitation of the Ulvales biomass. A BLAST search of the known ulvan lyase and the GH105 against the draft genome has found no matching sequences.…”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequence of Pseudoalteromonas sp. Strain PLSV, an Ulvan-Degrading Bacterium

et al. 2014

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“…These draft genome sequences of ulvan-utilizing bacteria, along with the already (9), Alteromonas spp. (10), and a Pseudoalteromonas sp. (11), will open up possibilities for the usefulness of polysaccharide ulvan from Ulva spp.…”

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confidence: 99%

Draft Genome Sequences of Bacterial Strains Capable of Degrading Ulvan from the Green Alga Ulva ohnoi

Mondal

Satoh

Ohnishi

2020

Microbiol Resour Announc

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Draft Genome Sequences of Two Ulvan-Degrading Isolates, Strains LTR and LOR, That Belong to the Alteromonas Genus

Abstract: Here, we report the draft genome sequence of two ulvan-degrading Alteromonas spp. isolated from the feces of the sea slug, Aplysia. These sequenced genomes display a unique ulvan degradation machinery compared with ulvanolytic enzymes previously identified in Nonlabens ulvanivorans.

Cited by 11 publications

References 12 publications

New Family of Ulvan Lyases Identified in Three Isolates from the Alteromonadales Order

New Family of Ulvan Lyases Identified in Three Isolates from the Alteromonadales Order

Draft Genome Sequence of Pseudoalteromonas sp. Strain PLSV, an Ulvan-Degrading Bacterium

Draft Genome Sequences of Bacterial Strains Capable of Degrading Ulvan from the Green Alga Ulva ohnoi

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