Draft Genome Sequence of
            <i>Pseudoalteromonas</i>
            sp. Strain PLSV, an Ulvan-Degrading Bacterium

Kopel, Moran; Helbert, William; Henrissat, Bernard; Doniger, Tirza; Banin, Ehud

doi:10.1128/genomea.01257-14

Cited by 7 publications

(9 citation statements)

References 14 publications

(15 reference statements)

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“…52 Ulvan lyase activity has also been reported in Pseudoalteromonas spp. 53 This study confirms the ulvanolytic activity of bacteria belonging to genus Pseudoalteromonas. Further study on the ulvan lyases produced by the organism isolated in this study will help us determine if the enzyme is the same or diversity exists in the enzyme produced by different species from the same genus.…”

Section: Discussionsupporting

confidence: 78%

Diversity of Ulvan and Cellulose Depolymerizing Bacteria Associated With the Green Macroalgae Ulva Spp

Odaneth¹

2017

JABB

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Ulva spp. is a green macroalgae with high cell wall polysaccharide content. Ulvan and cellulose are the major polysaccharides present in Ulva spp. breakdown products of which have potential applications in the biofuel and fine chemical industry. Bacteria attached to Ulva spp. potentially possess enzymes that have the capability to depolymerize these polysaccharides. Ulvan and cellulose depolymerizing bacterial communities associated with fresh and rotting green macroalgae Ulva spp. collected from Aberystwyth shore (Wales, United Kingdom) were studied with the aim of understanding the breakdown of these polysaccharides in nature. Polysaccharide breakdown capability was investigated by the screening of these organisms for various enzymes involved in the breakdown of cellulose and ulvan. Positive organisms were identified using the 16S rRNA homology approach by amplification and sequencing of their 16S rRNA gene using universal primers, followed by homology analysis using NCBI BLAST. Phylum Proteobacteria with class Gammaproteobacteria predominated the community and was represented by species of orders Oceanospirillales, Vibrionales, Alteromonadales and phylum Bacteroidetes was represented singly by a species belonging to order Flavobacteriales. A synergistic mechanism was observed towards the breakdown of these polysaccharides with different sets of organisms contributing towards different enzymes required for the complete saccharification of ulvan and cellulose..

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Section: Discussionsupporting

confidence: 78%

Diversity of Ulvan and Cellulose Depolymerizing Bacteria Associated With the Green Macroalgae Ulva Spp

Odaneth¹

2017

JABB

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“…LTR, and Pseudoalteromonas sp. PLSV, (8,12,13). Genome annotation was manually confirmed by CAZy 4 and revealed that these strains encoded many polysaccharide-degrading enzymes.…”

Section: Table 2 Primers Used In This Studymentioning

confidence: 99%

“…LTR, and Pseudoalteromonas sp. PLSV (12,13). Inspection of the genomes did not reveal genes encoding proteins homologous to N. ulvanivorans ulvan lyase.…”

mentioning

confidence: 96%

New Family of Ulvan Lyases Identified in Three Isolates from the Alteromonadales Order

Kopel

Helbert

Belnik

et al. 2016

Journal of Biological Chemistry

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Ulvan is the main polysaccharide component of the Ulvales (green seaweed) cell wall. It is composed of disaccharide building blocks comprising 3-sulfated rhamnose linked to D-glucuronic acid (GlcUA), L-iduronic acid (IdoUA), or D-xylose (Xyl). The degradation of ulvan requires ulvan lyase, which catalyzes the endolytic cleavage of the glycoside bond between 3-sulfated rhamnose and uronic acid according to a ␤-elimination mechanism. The first characterized ulvan lyase was identified in Nonlabens ulvanivorans, an ulvanolytic bacterial isolate. In the current study, we have identified and biochemically characterized novel ulvan lyases from three Alteromonadales isolated bacteria. Two homologous ulvan lyases (long and short) were found in each of the bacterial genomes. The protein sequences have no homology to the previously reported ulvan lyases and therefore are the first representatives of a new family of polysaccharide lyases. The enzymes were heterologously expressed in Escherichia coli to determine their mode of action. The heterologous expressed enzymes were secreted into the milieu subsequent to their signal sequence cleavage. An endolytic mode of action was observed and studied using gel permeation chromatography and 1 H NMR. In contrast to N. ulvanivorans ulvan lyase, cleavage occurred specifically at the GlcUA residues. In light of the genomic context and modular structure of the ulvan lyase families identified to date, we propose that two ulvan degradation pathways evolved independently.

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“…(10), and a Pseudoalteromonas sp. (11), will open up possibilities for the usefulness of polysaccharide ulvan from Ulva spp. Data availability.…”

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confidence: 99%

Draft Genome Sequences of Bacterial Strains Capable of Degrading Ulvan from the Green Alga Ulva ohnoi

Mondal

Satoh

Ohnishi

2020

Microbiol Resour Announc

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Draft Genome Sequence of Pseudoalteromonas sp. Strain PLSV, an Ulvan-Degrading Bacterium

Abstract: We present the draft genome sequence of Pseudoalteromonas sp. strain PLSV, isolated from the feces of an Aplysia sea slug. The addition of the PLSV genome to the existing genomes of three other ulvan-degrading bacterial species will enhance our understanding of ulvan utilization.

Cited by 7 publications

References 14 publications

Diversity of Ulvan and Cellulose Depolymerizing Bacteria Associated With the Green Macroalgae Ulva Spp

Diversity of Ulvan and Cellulose Depolymerizing Bacteria Associated With the Green Macroalgae Ulva Spp

New Family of Ulvan Lyases Identified in Three Isolates from the Alteromonadales Order

Draft Genome Sequences of Bacterial Strains Capable of Degrading Ulvan from the Green Alga Ulva ohnoi

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