Draft Genome Sequences of Three Francisella tularensis subsp.
 mediasiatica
 Strains Isolated in the Altai Territory, Russian Federation

Мокриевич, А. Н.; Kislichkina, Angelina A.; Kudryavtseva, T. Yu.; Mironova, R. I.; Vakhrameeva, G. M.; Shishkova, N.A.; Timofeev, Vitalii; Богун, А. Г.; Павлов, В. М.; Dyatlov, I. A.

doi:10.1128/mra.01202-19

Cited by 3 publications

(4 citation statements)

References 5 publications

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“…Nine among these strains were previously analyzed by multiple locus variable number of tandem repeats (VNTR) analysis (MLVA) [2], the last four are new. WGS data from three strains were previously published [2,5]. Strains of Central Asian (M.I) and Altaic (M.II) origin form two clusters separated by 116 SNPs (Figure 3) in agreement with previous analyses [2,11].…”

Section: Whole-genome Sequencing (Wgs) and Phylogenetic Analysissupporting

confidence: 89%

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The First Finding of Francisella Tularensis Subsp. Mediasiatica in Krasnoyarsk Territory, Siberia and an Update of the Subspecies Genetic Diversity

Timofeev¹,

Бахтеева²,

Мокриевич³

et al. 2022

Preprint

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Tularemia is a severe infectious disease caused by the Gram-negative bacteria Francisella tularensis. F. tularensis is currently divided into three subspecies, holarctica, tularensis and mediasiatica which differ in their virulence and geographic distribution. Subspecies mediasiatica is the least studied because of its very low documented virulence for humans and limited geographic distribution. It was discovered in sparsely populated regions of Central Asia. Since 2011, a new subsp. mediasiatica lineage was identified in Altai (Russia). In 2021, we isolated one subsp. mediasiatica strain in Krasnoyarsk Territory. In spite of its geographic origin, 500 km east from Altai, this strain belongs to the Altai lineage and contributes surprisingly little genetic diversity to previous knowledge. This improved knowledge of the phylogeography of subsp. mediasiatica led us to propose a scenario in which the two zoonotic lineages, holarctica and tularensis, independently emerged from the mediasiatica lineage in Siberia or Central Asia, and make predictions which will allow to challenge this hypothesis

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Section: Whole-genome Sequencing (Wgs) and Phylogenetic Analysissupporting

confidence: 89%

“…WGS data from newly published genomes are available at https://www.ncbi.nlm.nih.gov/sra/PRJNA870100. Data for previously described genomes are available at the link indicated in [5]…”

Section: Dna Preparation and Pcr Analysis Of Species And Subspeciesmentioning

confidence: 99%

The First Finding of Francisella Tularensis Subsp. Mediasiatica in Krasnoyarsk Territory, Siberia and an Update of the Subspecies Genetic Diversity

Timofeev¹,

Бахтеева²,

Мокриевич³

et al. 2022

Preprint

View full text Add to dashboard Cite

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“…F. tularensis subspecies tularensis (Type A) is further separated into two distinct genetic groups, A.I and A.II, with three major branches in A.I [ 67 , 68 ] and two major branches in A.II [ 69 , 70 ]. F. tularensis subspecies mediasiatica is divided into clades, M.I and M.II, wherein M.II is represented by three recently available genomes from Russia [ 71 ]. A third clade, M.III, has been proposed but no genomes are available for this clade [ 5 ].…”

Section: Resultsmentioning

confidence: 99%

Reorganized Genomic Taxonomy of Francisellaceae Enables Design of Robust Environmental PCR Assays for Detection of Francisella tularensis

et al. 2021

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In recent years, an increasing diversity of species has been recognized within the family Francisellaceae. Unfortunately, novel isolates are sometimes misnamed in initial publications or multiple sources propose different nomenclature for genetically highly similar isolates. Thus, unstructured and occasionally incorrect information can lead to confusion in the scientific community. Historically, detection of Francisella tularensis in environmental samples has been challenging due to the considerable and unknown genetic diversity within the family, which can result in false positive results. We have assembled a comprehensive collection of genome sequences representing most known Francisellaceae species/strains and restructured them according to a taxonomy that is based on phylogenetic structure. From this structured dataset, we identified a small number of genomic regions unique to F. tularensis that are putatively suitable for specific detection of this pathogen in environmental samples. We designed and validated specific PCR assays based on these genetic regions that can be used for the detection of F. tularensis in environmental samples, such as water and air filters.

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“…Twelve among the 16 Altai strains were previously analyzed by a multiple-locus variable number of tandem repeats (VNTR) analysis (MLVA) [4], while four were new (A-87, A-178, A-196, A-77). WGS data from three strains were previously published [4,6]. We also included five public WGS datasets derived from Central Asian strains (listed in Figure 3).…”

Section: Whole-genome Sequencing (Wgs) and Phylogenetic Analysismentioning

confidence: 99%

The First Finding of Francisella tularensis subsp. mediasiatica in Krasnoyarsk Territory, Siberia, and an Update of the Subspecies Genetic Diversity

et al. 2022

View full text Add to dashboard Cite

Tularemia is a severe infectious disease caused by the Gram-negative bacteria Francisella tularensis. F. tularensis is currently divided into three subspecies, holarctica, tularensis, and mediasiatica, which differ in their virulence and geographic distribution. Subspecies mediasiatica is the least studied because of its very low documented virulence for humans and limited geographic distribution. It was discovered in sparsely populated regions of Central Asia. Since 2011, a new subsp. mediasiatica lineage was identified in Altai (Russia). In 2021, we isolated one subsp. mediasiatica strain in Krasnoyarsk Territory. In spite of its geographic origin, 500 km east from Altai, this strain belongs to the Altai lineage and contributes surprisingly little genetic diversity to previous knowledge.

show abstract

Draft Genome Sequences of Three Francisella tularensis subsp. mediasiatica Strains Isolated in the Altai Territory, Russian Federation

Abstract: We report the draft genome sequences of three Francisella tularensis subsp. mediasiatica strains isolated in the Altai Territory, Russian Federation.

Cited by 3 publications

References 5 publications

The First Finding of <em>Francisella Tularensis</em> Subsp. <em>Mediasiatica</em> in Krasnoyarsk Territory, Siberia and an Update of the Subspecies Genetic Diversity

The First Finding of <em>Francisella Tularensis</em> Subsp. <em>Mediasiatica</em> in Krasnoyarsk Territory, Siberia and an Update of the Subspecies Genetic Diversity

Reorganized Genomic Taxonomy of Francisellaceae Enables Design of Robust Environmental PCR Assays for Detection of Francisella tularensis

The First Finding of Francisella tularensis subsp. mediasiatica in Krasnoyarsk Territory, Siberia, and an Update of the Subspecies Genetic Diversity

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