The First Finding of Francisella tularensis subsp. mediasiatica in Krasnoyarsk Territory, Siberia, and an Update of the Subspecies Genetic Diversity

Timofeev, Vitalii; Бахтеева, И. В.; Мокриевич, А. Н.; Vakhrameeva, G. M.; Gritskova, Elena; Anisimov, Yuriy; Rozhdestvensky, Evgeny; Bazarova, Galina; Zhumakaev, Rostislav; Dyatlov, I. A.; Vergnaud, Gilles

doi:10.3390/bacteria1040018

Cited by 3 publications

(6 citation statements)

References 16 publications

(23 reference statements)

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“… Previously published short reads from sequence reads archive SRR21146809 [ 7 ] were mapped on the prmA gene to confirm the sequence derived from long reads. Position 1,245,071 is A instead of G in all 444 reads mapping on that position, causing a nonsense mutation within prmA .…”

Section: Resultsmentioning

confidence: 99%

“…The vast majority of subsp. mediasiatica strains were isolated from ticks Haemaphysalis concinna , Dermacentor silvarum and Ixodes persulcatus [ 7 ]. We are aware of only one strain isolated from wild rodents.…”

Section: Discussionmentioning

confidence: 99%

“…Whole chromosome comparisons for mutations search were run using the chromosome comparisons tool within BioNumerics version 8.1, with default parameters. Candidate nonsense mutations were checked by analysing previously published short reads sequence data using GeniousPrime version 2019.2.3 (bioproject PRJNA870100, sequence read archive SRR21146809) [ 7 , 12 ].…”

Section: Methodsmentioning

confidence: 99%

“…mediasiatica is significantly more geographically widespread than previously established. Subsp mediasiatica strains were found in a natural focus of tularemia in Altai (Russia) and more recently the same mediasiatica lineage was recovered in Siberia a few hundred kilometers east of the Altai focus [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…Genetic analysis of subsp mediasiatica strains of different geographical origin allowed to divide the subspecies into three very closely related subgroups: MI, represented by "classic" strains from Central Asia; MII, combining Altai and Siberian strains, and MIII. MIII subgroup is currently represented by only one strain 60(B)57 isolated in Karakalpakstan, Uzbekistan in 1960 [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

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Avirulence of a spontaneous Francisella tularensis subsp. mediasiatica prmA mutant

Timofeev,

Bakhteeva,

Titareva

et al. 2024

PLoS ONE

Self Cite

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Francisella tularensis, the causative agent of tularemia, is divided into three subspecies. Two of these, subspecies holarctica and tularensis, are highly pathogenic to humans and consequently relatively well studied. The third subspecies, mediasiatica, is rarely isolated and remains poorly studied. It is distributed in the sparsely populated regions of Central Asia and Siberia. Curently this subspecies is not known to have been responsible for human infections in spite of its high virulence in laboratory animals. Subspecies mediasiatica is currently divided into three subgroups—MI, present in Central Asia, MII, present in southern Siberia, and MIII represented by a unique strain, 60(B)57, isolated in Uzbekistan in 1960. We describe here the unexpected observation that MIII strain 60(B)57 is avirulent and immunogenic. We observed that infection with this strain protected mice from challenge 21 days later with a virulent subsp. mediasiatica strain. With an increase of this interval, the protection for mice was significantly reduced. In contrast, guinea pigs were protected from challenge with strains of the subspecies holarctica and mediasiatica (but not subsp. tularensis) 90 days after infection with 60(B)57. We performed genome assembly based on whole genome sequencing data obtained using the Nanopore MinION for strain 60(B)57 and two subsp. mediasiatica strains representing the Central Asian MI and Siberian MII phylogenetic subgroups. The prmA gene is truncated due to a nonsense mutation in strain 60(B)57. The deletion of gene prmA has previously been shown to induce a loss of virulence in Francisella novicida the closest model organism suggesting that the observed mutation might the cause of the avirulence of strain 60(B)57.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Avirulence of a spontaneous Francisella tularensis subsp. mediasiatica prmA mutant

Timofeev,

Bakhteeva,

Titareva

et al. 2024

PLoS ONE

Self Cite

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Genetic Homogeneity of Francisella tularensis subsp. mediasiatica Strains in Kazakhstan

Shevtsov,

Izbanova,

Amirgazin

et al. 2024

Pathogens

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Tularemia is an acute febrile disease caused by the Gram-negative bacillus Francisella tularensis. Based on genetic and phenotypic characteristics, three subspecies are distinguished: tularensis, holarctica, and mediasiatica. F. tularensis subsp. mediasiatica remains the least studied subspecies. Over the past decade, new foci of distribution of F. tularensis subsp. mediasiatica have been discovered in Russia (Siberia), expanding the possible distribution area by thousands of kilometers. This article provides whole genome single nucleotide polymorphism (wgSNP) and polymorphic tandem repeats (MLVA) analyses of 28 mediasiatica strains isolated between 1965 and 2004 in Kazakhstan. Despite high genetic homogeneity, MLVA with eleven loci (MLVA11) demonstrates a high discriminatory ability (diversity index, 0.9497). The topological structure of the trees based on wgSNP and MLVA is not comparable; however, clustering remains congruent for most outbreaks, with the exception of two strains from one outbreak that are identical in terms of wgSNP but differ at three tandem repeat loci. Based on wgSNP, the strains are assigned to one of the three currently known mediasiatica sublineages, lineage M.I, together with other historical strains maintained in collections in Russia and Sweden. wgSNP shows limited previously unknown genetic diversity, with the M.I lineage size being only 118 SNPs. The wgSNP genotype is not strongly correlated with year and place of isolation.

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Phylogenetic characteristics of strains of the causative agent of tularemia in the Rostov region and identification of erythromycin-sensitive genovariants

Sorokin,

Pavlovich,

Tsymbalistova

et al. 2024

A&Ch

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Актуальность. Изучение вопросов филогеографии туляремийного микроба представляет не только практический, но и научный интерес, так как позволяет оценить эволюционные процессы Francisella tularensis в современных условиях. Цель. Филогенетический анализ штаммов возбудителя туляремии на территории Ростовской области и выявление эритромициночувствительных геновариантов. Материал и методы. Культурально-морфологические, биохимические и биологические свойства туляремийного микроба изучали в соответствии с МУ 3.1.2007-05. Антибиотикочувствительность исследуемых культур изучали диско-диффузионным методом, согласно МУК 4.2.2495-09. Для генотипирования штаммов применён метод VNTR анализа по 5 локусам. Кластерный анализ и построение филогенетического дерева проводили с использованием программы GrapeTree. Результаты. Проведено исследование репрезентативной коллекции 122 культур, изолированных из различных источников в период с 1945 по 2022 гг. Как установлено, все изученные штаммы возбудителя туляремии обладали типичными культурально-морфологическими и биологическими характеристиками. Результаты изучения антибиотикограмм бактерий показали, что все штаммы были устойчивы к бета-лактамам, полимиксину, клиндамицину и чувствительны к аминогликозидам, рифампицину и фторхинолонам. В отношении эритромицина обнаружены только 2 эритромициночувствительных штамма. Филогенетический анализ 34 европейских и российских штаммов F. tularensis позволил определить принадлежность этих штаммов к подгруппе В.7 основной генетической группы В.6. Заключение. Впервые в Целинском районе Ростовской области зарегистрировано наличие «нетипичных» геновариантов туляремийного микроба, принадлежащих к подгруппе В.7 основной группы В.6 подвида holarctica, согласно современной схеме генетического типирования F. tularensis subsp. holarctica.

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The First Finding of Francisella tularensis subsp. mediasiatica in Krasnoyarsk Territory, Siberia, and an Update of the Subspecies Genetic Diversity

Cited by 3 publications

References 16 publications

Avirulence of a spontaneous Francisella tularensis subsp. mediasiatica prmA mutant

Avirulence of a spontaneous Francisella tularensis subsp. mediasiatica prmA mutant

Genetic Homogeneity of Francisella tularensis subsp. mediasiatica Strains in Kazakhstan

Phylogenetic characteristics of strains of the causative agent of tularemia in the Rostov region and identification of erythromycin-sensitive genovariants

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