Pasteurella multocida which is the common cause of respiratory disease in cattle causing high economic losses. To achieve this objective, this study succeeded in isolating a total of 27 of P. multocida isolates from 280 samples (9.6%). Lung tissues showed the highest percentage of P. multocida isolation (13.1%), followed by Nasal Swabs (5%). These isolates were confirmed microscopically to be P. multocida by staining with Gram's and Leishman's stains, then biochemically by traditional test like indole, catalase, oxidase, urease, citrate utilization, sugar fermentation and gelatin liquefaction tests. Also, the results were confirmed using commercial test API 20E and PCR. For common gene and capsular serotyping of P. multocida by using multiplex capsular PCR typing system, a total of 9 representative isolates were tested and 5 isolates were positive for common gene of P. multocida and positive for capsular type E. Positive P. multocida isolates were tested for presence of Phenotypic And Genotyping Characterization Of … Tamer I. Bostan et., al.