Draft Genome Sequences of Rhodotorula mucilaginosa Strains Isolated from the International Space Station

Daudu, Robert; Parker, Ceth W.; Singh, Nitin Kumar; Wood, Jason M.; Debieu, Marilyne; O’Hara, Niamh B.; Mason, Christopher E.; Venkateswaran, Kasthuri

doi:10.1128/mra.00570-20

Cited by 9 publications

(6 citation statements)

References 15 publications

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“…Rhodotorula is a genus of eukaryotic microorganisms in the family Sporidiobolaceae (order Sporidiales , class Pucciniomycetes , phylum Basidiomycota ) that is widely found in animals, plants, rivers, lakes, and oceans (Falces-Romero et al, 2016 ; Cudowski and Pietryczuk, 2019 ; Daudu et al, 2020 ). Cells of Rhodotorula not only are rich in conventional nutrients such as carotenoids, proteins, and polysaccharides but also contain amino acids, polyunsaturated fatty acids, vitamin E, nucleotides, and astaxanthin (Gupta et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Effects of Rhodotorula mucilaginosa on the Immune Function and Gut Microbiota of Mice

Ge¹,

Huang²,

Xie³

et al. 2021

Front. Fungal Biol.

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As a dominant species among marine yeasts, Rhodotorula benthica accounts for ~50% of all marine yeasts. Rhodotorula is rich in a variety of bioactive substances and commonly used in the production of carotenoids by microbial fermentation and is worth developing. Therefore, the present study used a strain of Rhodotorula mucilaginosa isolated from the coastal waters of the South China Sea as the target yeast to investigate its impact on the immune function and gut microbiota of mice. A total of 200 mice were randomly divided into gavage groups and control group and garaged for 30 consecutive days at different concentration. Samples were collected on day 15 and day 30 of gavage administration. The results showed that R. mucilaginosa ZTHY2 could increase the thymus and spleen indices of mice, and its effect on the thymus index was more significant after long-term gavage administration. Short-term (15 days) gavage administration of R. mucilaginosa suspension enhanced delayed hypersensitivity in mice, increased serum IgG, IgA, and IL-2. Long-term (30 days) gavage administration of R. mucilaginosa suspension significantly enhanced the phagocytosis of macrophages in mice and significantly increased serum TNF-α and INF-γ. R. mucilaginosa ZTHY2 altered the structure of the gut microbiota of mice at the phylum and genus levels, leading to an increased relative abundance of Firmicutes and Lactobacillus and a decreased relative abundance of Bacteroidetes. This strain increased the beneficial intestinal bacteria and reduced the harmful intestinal bacteria in mice. This study provides experimental evidence and lays the foundation for the future development and application of this strain as a microecological source of carotenoids.

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Section: Introductionmentioning

confidence: 99%

Effects of Rhodotorula mucilaginosa on the Immune Function and Gut Microbiota of Mice

Ge¹,

Huang²,

Xie³

et al. 2021

Front. Fungal Biol.

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“… Rhodotorula mucilaginosa is a ubiquitous environmental ( 22 ) and human commensal yeast and opportunistic pathogen ( 23 ) that is found in aquatic and built environments, including bathrooms and dishwashers ( 24 , 25 ). It is the most commonly isolated yeast on the ISS ( 26 – 28 ).…”

Section: Announcementmentioning

confidence: 99%

Draft Genome Sequences of Fungi Isolated from the International Space Station during the Microbial Tracking-2 Experiment

Simpson

Urbaniak

Bateh

et al. 2021

Microbiol Resour Announc

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“…Until the mid-2010s, monitoring of bacteria and fungi aboard the ISS was mainly done via traditional culturing and identification using Sanger sequencing methods, to ensure crew health and food and water safety (La Duc et al, 2004 ; Checinska et al, 2015 ). More recently, a series of NASA Space Biology-funded missions known as Microbial Tracking 1 and 2 investigated the metagenomes of ISS surfaces and performed WGS on all isolated bacteria and fungi (Checinska Sielaff et al, 2016 ; Venkateswaran et al, 2017 ; Urbaniak et al, 2018 ; Bijlani et al, 2020 ; Daudu et al, 2020 ; Solomon et al, 2020 ; Blachowicz et al, 2021 ; Simpson et al, 2021a , b ), in order to fully characterize the ISS microbiome and how it changes over time (Singh et al, 2018 ; Checinska Sielaff et al, 2019 ; Urbaniak et al, 2022 ). In the ongoing Microbial Tracking investigation of the ISS, spore-forming bacteria have been isolated multiple times from the genera Bacillus and Paenibacillus ; however, this is the first instance that a bacterium from the genus Cohnella has been isolated from an ISS surface.…”

Section: Introductionmentioning

confidence: 99%

Comparative genomic analysis of Cohnella hashimotonis sp. nov. isolated from the International Space Station

et al. 2023

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A single strain from the family Paenibacillaceae was isolated from the wall behind the Waste Hygiene Compartment aboard the International Space Station (ISS) in April 2018, as part of the Microbial Tracking mission series. This strain was identified as a gram-positive, rod-shaped, oxidase-positive, catalase-negative motile bacterium in the genus Cohnella, designated as F6_2S_P_1T. The 16S sequence of the F6_2S_P_1T strain places it in a clade with C. rhizosphaerae and C. ginsengisoli, which were originally isolated from plant tissue or rhizosphere environments. The closest 16S and gyrB matches to strain F6_2S_P_1T are to C. rhizosphaerae with 98.84 and 93.99% sequence similarity, while a core single-copy gene phylogeny from all publicly available Cohnella genomes places it as more closely related to C. ginsengisoli. Average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values to any described Cohnella species are <89 and <22%, respectively. The major fatty acids for strain F6_2S_P_1T are anteiso-C15:0 (51.7%), iso-C16:0 (23.1%), and iso-C15:0 (10.5%), and it is able to metabolize a wide range of carbon compounds. Given the results of the ANI and dDDH analyses, this ISS strain is a novel species within the genus Cohnella for which we propose the name Cohnella hashimotonis, with the type strain F6_2S_P_1T (=NRRL B-65657T and DSMZ 115098T). Because no closely related Cohnella genomes were available, this study generated the whole-genome sequences (WGSs) of the type strains for C. rhizosphaerae and C. ginsengisoli. Phylogenetic and pangenomic analysis reveals that F6_2S_P_1T, C. rhizosphaerae, and C. ginsengisoli, along with two uncharacterized Cohnella strains, possess a shared set of 332 gene clusters which are not shared with any other WGS of Cohnella species, and form a distinct clade branching off from C. nanjingensis. Functional traits were predicted for the genomes of strain F6_2S_P_1T and other members of this clade.

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Draft Genome Sequences of Rhodotorula mucilaginosa Strains Isolated from the International Space Station

Cited by 9 publications

References 15 publications

Effects of Rhodotorula mucilaginosa on the Immune Function and Gut Microbiota of Mice

Effects of Rhodotorula mucilaginosa on the Immune Function and Gut Microbiota of Mice

Draft Genome Sequences of Fungi Isolated from the International Space Station during the Microbial Tracking-2 Experiment

Comparative genomic analysis of Cohnella hashimotonis sp. nov. isolated from the International Space Station

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