Draft Genome Sequences of Four Propionibacterium acnes Strains Isolated from Implant-Related Infections

Aubin, Guillaume Ghislain; Kambarev, Stanimir; Guillouzouic, A.; Lepelletier, Didier; Bémer, Pascale; Corvec, Stéphane

doi:10.1128/genomea.01395-16

Cited by 5 publications

(1 citation statement)

References 15 publications

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“…WGS was performed on a subset of isolates to highlight candidate genes potentially involved in the differences in phenotypic behavior observed previously ( Table 1 ). The ATCC6919, BL, Ntes, HB, 2003-1719 and 2004-10708 strains yielded 2617, 2469, 2416, 2410, 2463 and 2410 predicted coding sequences (CDSs), respectively 31 32 . Slightly less than half (44 to 47%) of the CDSs in each strain could be functionally categorized into 315 to 331 subsystems.…”

Section: Resultsmentioning

confidence: 99%

Interaction of Cutibacterium ( formerly Propionibacterium) acnes with bone cells: a step toward understanding bone and joint infection development

Aubin

Baud’huin

Lavigne

et al. 2017

Sci Rep

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Cutibacterium acnes (formerly Propionibacterium acnes) is recognized as a pathogen in foreign-body infections (arthroplasty or spinal instrumentation). To date, the direct impact of C. acnes on bone cells has never been explored. The clade of 11 C. acnes clinical isolates was determined by MLST. Human osteoblasts and osteoclasts were infected by live C. acnes. The whole genome sequence of six isolates of this collection was analyzed. CC36 C. acnes strains were significantly less internalized by osteoblasts and osteoclasts than CC18 and CC28 C. acnes strains (p ≤ 0.05). The CC18 C. acnes ATCC6919 isolate could survive intracellularly for at least 96 hours. C. acnes significantly decreased the resorption ability of osteoclasts with a major impact by the CC36 strain (p ≤ 0.05). Genome analysis revealed 27 genes possibly linked to these phenotypic behaviors. We showed a direct impact of C. acnes on bone cells, providing new explanations about the development of C. acnes foreign-body infections.

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Section: Resultsmentioning

confidence: 99%