Bacteria and Biofilms as Natural Inhabitants of Our Body

Ray, Rina Rani; Lahiri, Dibyajit; Chatterjee, Abarna; Banerjee, Prateek

doi:10.1007/978-981-16-0745-5_3

Cited by 3 publications

(1 citation statement)

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“…The relationship between acne vulgaris and fluctuations in sex hormone level is well established [50] , and some data are now available on how the stress hormones affect C. acnes [29] , [30] . We focused our efforts primarily on C. acnes biofilms because this lifestyle appears to be predominant for bacteria especially in the skin microenvironment [51] , and C. acnes biofilms remain a relatively understudied object. Despite the lack of significant differences between hormone-treated and untreated C. acnes planktonic cultures, shown by the CV staining method, intrinsic processes induced by epinephrine were revealed using omics techniques.…”

Section: Discussionmentioning

confidence: 99%

Proteomic and transcriptomic analyses of Cutibacterium acnes biofilms and planktonic cultures in presence of epinephrine

Gannesen,

Schelkunov,

Ziganshin

et al. 2024

AIMSMICRO

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<abstract> Transcriptomic and proteomic analysis were performed on 72 h biofilms of the acneic strain <italic>Cutibacterium acnes</italic> and planktonic cultures in the presence of epinephrine. Epinephrine predominantly downregulated genes associated with various transporter proteins. No correlation was found between proteomic and transcriptomic profiles. In control samples, the expression of 51 proteins differed between planktonic cultures and biofilms. Addition of 5 nM epinephrine reduced this number, and in the presence of 5 µM epinephrine, the difference in proteomic profiles between planktonic cultures and biofilms disappeared. According to the proteomic profiling, epinephrine itself was more effective in the case of <italic>C. acnes</italic> biofilms and potentially affected the tricarboxylic acid cycle (as well as alpha-ketoglutarate decarboxylase Kgd), biotin synthesis, cell division, and transport of different compounds in <italic>C. acnes</italic> cells. These findings are consistent with recent research on <italic>Micrococcus luteus</italic>, suggesting that the effects of epinephrine on actinobacteria may be universal. </abstract>

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