Draft Genome Sequences of Elizabethkingia anophelis Strains R26
            <sup>T</sup>
            and Ag1 from the Midgut of the Malaria Mosquito Anopheles gambiae

Kukutla, Phanidhar; Lindberg, Bo G.; Pei, Dong; Rayl, Melanie; Yu, Wanqin; Steritz, Matthew J.; Faye, Ingrid; Xu, Jianchu

doi:10.1128/genomea.01030-13

Cited by 33 publications

(32 citation statements)

References 14 publications

(13 reference statements)

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“…We found various antimicrobial resistance genes consistent with their resistance phenotypes, including metallo-β-lactamase ( bla GOB-1 and blaB14 in strain HKU36 and a novel bla GOB and blaB1 in strains HKU37 and HKU38) and extended-spectrum β-lactamase ( blaA CME-1 in strains HKU37 and HKU38 and a potential novel blaA CME-1 variant in strain HKU36). A comparison of these β-lactamases to their corresponding orthologs in E. meningoseptica genomes revealed only 74%–85% amino acid identities, indicating that E. anophelis and related bacteria are potential reservoirs of novel β-lactamase genes ( 19 , 34 , 35 ) . Other antimicrobial resistance genes found included multidrug-resistance efflux pumps (ATP binding cassette superfamily, major facilitator superfamily, resistance-nodulation-division families, multidrug and toxic-compound extrusion family) that potentially carry resistance to a variety of compounds; chloramphenicol acetyltransferase; aminoglycoside 6-adenyltransferase; and tetracycline resistant gene.…”

Section: Resultsmentioning

confidence: 99%

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Evidence forElizabethkingia anophelisTransmission from Mother to Infant, Hong Kong

Lau¹,

Wu²,

Teng³

et al. 2015

Emerg. Infect. Dis.

118

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Section: Resultsmentioning

confidence: 99%

“…E. anophelis strains R26 T and Ag1 were isolated from mosquitoes ( 35 ). Compared with those strains , the genomes of the 3 strains we identified possessed 33 unique hypothetical proteins.…”

Section: Resultsmentioning

confidence: 99%

Evidence forElizabethkingia anophelisTransmission from Mother to Infant, Hong Kong

Lau¹,

Wu²,

Teng³

et al. 2015

Emerg. Infect. Dis.

118

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“…Recently, the genomes of several mosquito derived bacterial strains have been sequences, including Asaia sp., Elizabethkingia anophelis , Enterobacter sp., Pseudomonas sp., Serratia sp., Stenotrophomonas maltophila , and Staphylococcus hominis 55565758596061. These genome data allow one to analyse the microbial genetic repertoire that may be associated with symbiosis.…”

Section: Discussionmentioning

confidence: 99%

Diversity of bacteriome associated with Phlebotomus chinensis (Diptera: Psychodidae) sand flies in two wild populations from China

Chen

Jiang

et al. 2016

Sci Rep

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Sand fly Phlebotomus chinensis is a primary vector of transmission of visceral leishmaniasis in China. The sand flies have adapted to various ecological niches in distinct ecosystems. Characterization of the microbial structure and function will greatly facilitate the understanding of the sand fly ecology, which would provide critical information for developing intervention strategy for sand fly control. In this study we compared the bacterial composition between two populations of Ph. chinensis from Henan and Sichuan, China. The phylotypes were taxonomically assigned to 29 genera of 19 families in 9 classes of 5 phyla. The core bacteria include Pseudomonas and enterobacteria, both are shared in the sand flies in the two regions. Interestingly, the endosymbionts Wolbachia and Rickettsia were detected only in Henan, while the Rickettsiella and Diplorickettsia only in Sichuan. The intracellular bacteria Rickettsia, Rickettsiella and Diplorickettsia were reported for the first time in sand flies. The influence of sex and feeding status on the microbial structure was also detected in the two populations. The findings suggest that the ecological diversity of sand fly in Sichuan and Henan may contribute to shaping the structure of associated microbiota. The structural classification paves the way to function characterization of the sand fly associated microbiome.

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“…Previous several studies have also reported a similar pattern of gut microbe enrichment (Tchioffo, Boissiere et al 2015;Muturi, Dunlap et al 2019), but nature of gutmicrobe interactions, especially microbial proteins facilitating blood meal digestion, remains poorly known (Chen, Blom et al 2017). Available draft genome sequence of cultured bacterial species predicts several metabolic pathways, but no functional relation has been established (Kukutla, Lindberg et al 2013;Pei, Hill-Clemons et al 2015).…”

Section: Fig 8 Relative Quantification Of Gut Immune Transcripts Inmentioning

confidence: 99%

Altered gut microbiota and immunity defines Plasmodium vivax survival in Anopheles stephensi

Sharma

Rani

Chauhan

et al. 2019

Preprint

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AbstractBlood feeding-enriched gut-microbiota boosts mosquitoes’ anti-Plasmodium immunity. Here, we ask how Plasmodium vivax alters microbiota, anti-Plasmodial immunity and impact tripartite Plasmodium-mosquito-microbiota interactions in the gut lumen. Using a metagenomics analysis, we predominantly detect Elizabethkingia meningitis and Pseudomonas sps. in naïve mosquitoes. Naïve blood fed gut shows a heightened presence of Elizabethkingia, Pseudomonas and Serratia. A parallel RNAseq analysis of blood-fed midguts identify Elizabethkingia-transcripts, which may have role in iron metabolism. Post, a Plasmodium vivax infected blood-meal, however, we do not detect bacterial until circa 36 hours. Intriguingly, transcriptional expression of a selected array of antimicrobial arsenal cecropins 1-2, defensin-1 and gambicin remains low during the first 36 hours–a time frame when ookinietes/early oocysts invade gut. We conclude during the preinvasive phase, Plasmodium vivax outcompetes midgut-microbiota. Suppression of important immune factors, likely due to altered microbiota, may enhance Plasmodium vivax survival. Additional finding of a novel Wolbachia association warrants further research to design ‘paratransgenesis’ tools for malaria control.Author SummarySuccessful malaria transmission relies on the competitive interactions of Plasmodium and mosquito’s tissue specific immune potential. Within 24hrs of blood meal gut-microbiota grows exponentially and lead to robust enhancement of mosquito immune response, which is detrimental to parasite survival and development. But the mechanism how Plasmodium manages to evade this pre-invasive immune barrier is not well known. We investigated the influence of tripartite gut-microbiome-parasite interaction on human malaria parasite Plasmodium vivax in its natural/native vector Anopheles stephensi. Surprisingly we found that infectious blood meal lead to dramatic suppression in gut-bacteria population, a plausible strategy of P. vivax ookinetes to avoid immune responses. Our study suggests that for its own survival Plasmodium vivax causes early suppression of bacterial population, possibly by scavenging Fe from the blood meal which is indispensable for bacterial growth. Disruption and manipulation of this gut-microbe-interaction may help to design new ‘paratransgenesis’ molecular tool for malaria control.

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Draft Genome Sequences of Elizabethkingia anophelis Strains R26 ^T and Ag1 from the Midgut of the Malaria Mosquito Anopheles gambiae

Cited by 33 publications

References 14 publications

Evidence forElizabethkingia anophelisTransmission from Mother to Infant, Hong Kong

Evidence forElizabethkingia anophelisTransmission from Mother to Infant, Hong Kong

Diversity of bacteriome associated with Phlebotomus chinensis (Diptera: Psychodidae) sand flies in two wild populations from China

Altered gut microbiota and immunity defines Plasmodium vivax survival in Anopheles stephensi

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Draft Genome Sequences of Elizabethkingia anophelis Strains R26 T and Ag1 from the Midgut of the Malaria Mosquito Anopheles gambiae

Cited by 33 publications

References 14 publications

Evidence forElizabethkingia anophelisTransmission from Mother to Infant, Hong Kong

Evidence forElizabethkingia anophelisTransmission from Mother to Infant, Hong Kong

Diversity of bacteriome associated with Phlebotomus chinensis (Diptera: Psychodidae) sand flies in two wild populations from China

Altered gut microbiota and immunity defines Plasmodium vivax survival in Anopheles stephensi

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Product

Resources

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Draft Genome Sequences of Elizabethkingia anophelis Strains R26 ^T and Ag1 from the Midgut of the Malaria Mosquito Anopheles gambiae