Diversity of bacteriome associated with Phlebotomus chinensis (Diptera: Psychodidae) sand flies in two wild populations from China

Li, Kaili; Chen, Huiying; Jiang, Jinjin; Li, Xiangyu; Xu, Jianchu; Ma, Yajun

doi:10.1038/srep36406

Cited by 35 publications

(34 citation statements)

References 67 publications

(71 reference statements)

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“…Three of these new genera -Pluralibacter, Pseudocitrobacter, and Rosenbergiella -belong to the Enterobacteriaceae. Pluralibacter was recently differentiated from the Enterobacter genus (Brady et al, 2013) and was also found in the sand fly Phlebotomus chinensis Newstead (Li et al, 2016), the Mediterranean fruit fly (medfly), C. capitata (Papanicolaou et al, 2016), and the mosquito Anopheles albimanus Wiedemann (Dada et al, 2018). In contrast, Pseudocitrobacter and Rosenbergiella have never been reported as insect gut microbes before.…”

Section: Discussionmentioning

confidence: 99%

The effects of geographic origin and antibiotic treatment on the gut symbiotic communities ofBactrocera oleaepopulations

Koskinioti

Ras

Augustinos

et al. 2019

Entomologia Exp Applicata

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The olive fruit fly, Bactrocera oleae (Rossi) (Diptera: Tephritidae), is the major insect pest of olive orchards (Olea europaea L.), causing extensive damages on cultivated olive crops worldwide. Due to its economic importance, it has been the target species for a variety of population control approaches including the sterile insect technique (SIT). However, the inefficiency of the current mass‐rearing techniques impedes the successful application of area‐wide integrated pest management programs with an SIT component. It has been shown that insect mass rearing and quality of sterile insects can be improved by the manipulation of the insect gut microbiota and probiotic applications. In order to exploit the gut bacteria, it is important to investigate the structure of the gut microbial community. In the current study, we characterized the gut bacterial profile of two wild olive fruit fly populations introduced in laboratory conditions using next generation sequencing of two regions of the 16S rRNA gene. We compared the microbiota profiles regarding the geographic origin of the samples. Additionally, we investigated potential changes in the gut bacteria community before and after the first exposure of the wild adult flies to artificial adult diet with and without antibiotics. Various genera – such as Erwinia, Providencia, Enterobacter, and Klebsiella – were detected for the first time in B. oleae. The most dominant species was Candidatus Erwinia dacicola Capuzzo et al. and it was not affected by the antibiotics in the artificial adult diet used in the first generation of laboratory rearing. Geographic origin affected the overall structure of the gut community of the olive fruit fly, but antibiotic treatment in the first generation did not significantly alter the gut microbiota community.

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Section: Discussionmentioning

confidence: 99%

The effects of geographic origin and antibiotic treatment on the gut symbiotic communities ofBactrocera oleaepopulations

Koskinioti

Ras

Augustinos

et al. 2019

Entomologia Exp Applicata

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“…The majority of bacteria identified by sequencing a 16S rDNA clone library obtained from the gut contents of adult females of this species were found to be from the families Coxiellaceae, Enterobacteriaceae mostly from the genus Enterococcus , and Pseudomonadaceae mostly from the genus Pseudomonas . Additionally, the intracellular Diplorickettsia , Rickettsia , Rickettsiella , Spiroplasma and Wolbachia were identified with these methods in the same study (Li et al , 2016 ). Curiously, Rickettsia and Wolbachia were found only in sand flies collected from a locality where anthroponotic visceral leishmaniasis occurs and dogs and other animals are the reservoirs (Li et al , 2016 ).…”

Section: Sand Fly Microbiotamentioning

confidence: 97%

“…Additionally, the intracellular Diplorickettsia , Rickettsia , Rickettsiella , Spiroplasma and Wolbachia were identified with these methods in the same study (Li et al , 2016 ). Curiously, Rickettsia and Wolbachia were found only in sand flies collected from a locality where anthroponotic visceral leishmaniasis occurs and dogs and other animals are the reservoirs (Li et al , 2016 ). Diplorickettsia , Rickettsiella and Spiroplasma were found only in sand flies collected from a locality where zoonotic visceral leishmaniasis occurs and humans are the identified reservoirs.…”

Section: Sand Fly Microbiotamentioning

confidence: 97%

Leishmania, microbiota and sand fly immunity

et al. 2018

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In this review, we explore the state-of-the-art of sand fly relationships with microbiota, viruses and Leishmania, with particular emphasis on the vector immune responses. Insect-borne diseases are a major public health problem in the world. Phlebotomine sand flies are proven vectors of several aetiological agents including viruses, bacteria and the trypanosomatid Leishmania, which are responsible for diseases such as viral encephalitis, bartonellosis and leishmaniasis, respectively. All metazoans in nature coexist intimately with a community of commensal microorganisms known as microbiota. The microbiota has a fundamental role in the induction, maturation and function of the host immune system, which can modulate host protection from pathogens and infectious diseases. We briefly review viruses of public health importance present in sand flies and revisit studies done on bacterial and fungal gut contents of these vectors. We bring this information into the context of sand fly development and immune responses. We highlight the immunity mechanisms that the insect utilizes to survive the potential threats involved in these interactions and discuss the recently discovered complex interactions among microbiota, sand fly, Leishmania and virus. Additionally, some of the alternative control strategies that could benefit from the current knowledge are considered.

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“…Many sand y species are known to rely on a broad range of species for blood meals (19,20,(27)(28)(29) , typically mammals (20,(30)(31)(32)(33) but also various cold-blooded animals (21). In this study, we investigated the blood sources of sand ies by ampli ed speci c animal's mtDNA cyt b fragments using PCR.…”

Section: Discussionmentioning

confidence: 99%

Leishmania infection and blood sources analysis in Phlebotomus chinensis (Diptera: Psychodidae) along extension region of the Loess Plateau, China

Chen¹,

Feng²,

Gao³

et al. 2020

Preprint

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Background Visceral leishmaniasis (VL) was one of the most important parasitic diseases in China, caused by Leishmania protozoans and transmitted by sand flies. Recently VL cases have reappeared in China, including the extension region of the Loess Plateau. The purpose of this study was to collect fundamental data on the host-vector VL system in the Loess Plateau to assist in the development of prevention and control measures.Methods Sand flies were collected by light traps from rural areas in Shanxian, Henan, China in 2015, as well as in Wuxiang and Yangquan, Shanxi, China in 2017. The blood sources of sand flies were analyzed by PCR detecting the host-specific mitochondrial cytochrome b (mtDNA Cyt b) gene fragments. Leishmania infection in sand flies was detected by amplifying and sequencing ribosomal DNA internal transcribed spacer 1 (ITS1). The Leishmania specific antibodies in the sera of local dogs were detected by ELISA kit. Results Blood sources showed diversity in the extension region of the Loess Plateau, including human, chicken, dog, cattle, pig and goat. Multiple blood sources within a sand fly were observed in samples from Yangquan (17/118, 14.4%) and Wuxiang (12/108, 11.1%). Leishmania DNA was detected in sand flies collected from Yangquan with minimum infection rate of 1.00%. The ITS1 sequences were conserved with the Leishmania donovani complex. The positive rate of Leishmania specific antibodies in dogs was 5.97%. Conclusions This study detected the blood sources and Leishmania parasites infection of sand flies by molecular methods in the extension region of Loess Plateau, China. A high epidemic risk of leishmaniasis is currently indicated by the results as the infection of Leishmania in sand flies, the extensive blood sources of sand flies including humans, and positive antibody of Leishmania in local dog sera. Given the recent increase of VL cases, asymptomatic patients, dogs and other potential infected animals should be screened and treated. Furthermore, the density of sand flies needs to be controlled and personal protection should be strengthened.

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Diversity of bacteriome associated with Phlebotomus chinensis (Diptera: Psychodidae) sand flies in two wild populations from China

Cited by 35 publications

References 67 publications

The effects of geographic origin and antibiotic treatment on the gut symbiotic communities ofBactrocera oleaepopulations

The effects of geographic origin and antibiotic treatment on the gut symbiotic communities ofBactrocera oleaepopulations

Leishmania, microbiota and sand fly immunity

Leishmania infection and blood sources analysis in Phlebotomus chinensis (Diptera: Psychodidae) along extension region of the Loess Plateau, China

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