Biological control against malaria and its transmission is currently a considerable challenge. plantassociated bacteria of the genus Asaia are frequently found in nectarivorous arthropods, they thought to have a natural indirect action on the development of plasmodium in mosquitoes. However, virtually nothing is known about its natural cycle. Here, we show the role of nectar-producing plants in the hosting and dissemination of Asaia. We isolated Asaia strains from wild mosquitoes and flowers in Senegal and demonstrated the transmission of the bacteria from infected mosquitoes to sterile flowers and then to 26.6% of noninfected mosquitoes through nectar feeding. Thus, nectar-producing plants may naturally acquire Asaia and then colonize Anopheles mosquitoes through food-borne contamination. finally, Asaia may play an indirect role in the reduction in the vectorial capacity of Anopheles mosquitoes in a natural environment (due to Plasmodium-antagonistic capacities of Asaia) and be used in the development of tools for Asaia-based paratransgenetic malaria control.Nectarivory is extremely common in insects. Nectar is a sugar-rich liquid specially produced by plant glands (nectaries) to attract pollinating animals. The majority of nectarivorous insect species belong to the families Diptera, Coleoptera, Lepidoptera, Hymenoptera and Hemiptera. Nectar consumption has been established for many lower dipteran taxa, including not only the Tipulidae (crane flies), Cecidomyiidae (gall midges), Sciaridae (root gnats) and Mycetophilidae but also blood-feeding species, such as Ceratopogonidae (biting midges) and Culicidae (mosquitoes) 4 . The Diptera possess an elongated tube, which has characteristic morphological and functional adaptations to feed on either floral nectar (e.g., Bombyliidae, Syrphidae, and Nemestrinidae), vertebrate blood (e.g., Tabanidae and Glossinidae) or both (Culicidae, Simuliidae, and Ceratopogonidae) 5 .Male and female mosquitoes require sugar to obtain the energy they need to survive in their natural environment. Their sugar requirements are generally fulfilled from flower nectar near larval breeding sites or around adult resting sites. Plant nectar is a nutritional supplement that females often use between blood meals and when hosts are absent or rare. It is also used as an energy source and thus allows both male and female mosquitoes to survive in their environment 6 . Thus, plants play a very important role in mosquito survival in their natural environment, providing them with an easily accessible meal, which increases their life span and reduces contact with humans 6 . Plants also provide male mosquitoes with enough energy to successfully fertilize females, thus ensuring species perennity 7 . Mosquitoes are attracted by the odors emitted by plants and use those odors to locate plants that can provide the sugar and/or chemicals they need to survive in their natural environment 6 .Several studies have discussed the importance of arthropod vector microbiota for the prevention of certain vector-borne dis...