Draft Genome Sequences of Burkholderia contaminans FFI-28, a Strain Isolated from a Contaminated Pharmaceutical Solution

Haim, María Sol; Mollerach, Marta; Domselaar, Gary Van; Teves, S.; Degrossi, José; Cardona, Silvia T.

doi:10.1128/genomea.01177-16

Cited by 6 publications

(1 citation statement)

References 18 publications

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“…Bcc bacteria are known to survive in pharmaceutical and disinfectant materials ( 1 , 48 , 49 ). B. stabilis strains sharing MLST types can be isolated from the natural environment, hospitals, and patients ( 50 ), implicating the natural environment as a source of opportunistic Burkholderia and emphasizing the versatility of Bcc to survive and grow under diverse conditions.…”

Section: Discussionmentioning

confidence: 99%

Phenotypic and Genomic Analyses ofBurkholderia stabilisClinical Contamination, Switzerland

Seth-Smith¹,

Casanova²,

Sommerstein³

et al. 2019

Emerg. Infect. Dis.

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A recent hospital outbreak related to premoistened gloves used to wash patients exposed the difficulties of defining Burkholderia species in clinical settings. The outbreak strain displayed key B. stabilis phenotypes, including the inability to grow at 42°C; we used whole-genome sequencing to confirm the pathogen was B. stabilis . The outbreak strain genome comprises 3 chromosomes and a plasmid, sharing an average nucleotide identity of 98.4% with B. stabilis ATCC27515 BAA-67, but with 13% novel coding sequences. The genome lacks identifiable virulence factors and has no apparent increase in encoded antimicrobial drug resistance, few insertion sequences, and few pseudogenes, suggesting this outbreak was an opportunistic infection by an environmental strain not adapted to human pathogenicity. The diversity among outbreak isolates (22 from patients and 16 from washing gloves) is only 6 single-nucleotide polymorphisms, although the genome remains plastic, with large elements stochastically lost from outbreak isolates.

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Section: Discussionmentioning

confidence: 99%

Phenotypic and Genomic Analyses ofBurkholderia stabilisClinical Contamination, Switzerland

Seth-Smith¹,

Casanova²,

Sommerstein³

et al. 2019

Emerg. Infect. Dis.

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Whole Genome Sequence Analysis of Burkholderia contaminans FFH2055 Strain Reveals the Presence of Putative β-Lactamases

et al. 2019

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Burkholderia contaminans is a member of the Burkholderia cepacia complex (Bcc), a pathogen with increasing prevalence among cystic fibrosis (CF) patients and the cause of numerous outbreaks due to the use of contaminated commercial products. The antibiotic resistance determinants, particularly β-lactamases, have been poorly studied in this species. In this work, we explored the whole genome sequence (WGS) of a B. contaminans isolate (FFH 2055) and detected four putative β-lactamase-encoding genes. In general, these genes have more than 93% identity with β-lactamase genes found in other Bcc species. Two β-lactamases, a class A (Pen-like, suggested name PenO) and a class D (OXA-like), were further analyzed and characterized. Amino acid sequence comparison showed that Pen-like has 82% and 67% identity with B. multivorans PenA and B. pseudomallei PenI, respectively, while OXA-like displayed strong homology with class D enzymes within the Bcc, but only 22–44% identity with available structures from the OXA family. PCR reactions designed to study the presence of these two genes revealed a heterogeneous distribution among clinical and industrial B. contaminans isolates. Lastly, blaPenO gene was cloned and expressed into E. coli to investigate the antibiotic resistance profile and confers an extended-spectrum β-lactamase (ESBL) phenotype. These results provide insight into the presence of β-lactamases in B. contaminans, suggesting they play a role in antibiotic resistance of these bacteria.

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Prevalencia de especies del complejo Burkholderia cepacia en pacientes con fibrosis quística en Argentina durante el período 2011-2015

Cipolla

Rocca

Martínez

et al. 2018

Enfermedades Infecciosas y Microbiología Clínica

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Draft Genome Sequences of Burkholderia contaminans FFI-28, a Strain Isolated from a Contaminated Pharmaceutical Solution

Cited by 6 publications

References 18 publications

Phenotypic and Genomic Analyses ofBurkholderia stabilisClinical Contamination, Switzerland

Phenotypic and Genomic Analyses ofBurkholderia stabilisClinical Contamination, Switzerland

Whole Genome Sequence Analysis of Burkholderia contaminans FFH2055 Strain Reveals the Presence of Putative β-Lactamases

Prevalencia de especies del complejo Burkholderia cepacia en pacientes con fibrosis quística en Argentina durante el período 2011-2015

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