Draft Genome Sequence of the
            <scp>d</scp>
            -Xylose-Fermenting Yeast
            <i>Spathaspora arborariae</i>
            UFMG-HM19.1A
            <sup>T</sup>

Lobo, Francisco Pereira; Gonçalves, Davi L.; Alves, Sérgio L.; Gerber, Alexandra Lehmkuhl; Vasconcelos, Ana Tereza Ribeiro de; Basso, Luiz Carlos; Franco, Glória Regina; Soares, Marco A.; Cadete, Raquel M.; Rosa, Carlos A.; Stambuk, Boris U.

doi:10.1128/genomea.01163-13

Cited by 14 publications

(9 citation statements)

References 18 publications

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“…This “make-accumulate-consume” strategy provides a powerful ecological advantage by exploiting the rich reserves of simple sugars in sap, fruit, and other sources [30–33]. Other genera, such as Scheffersomyces and Spathaspora , have adapted to living in the guts of wood-consuming beetles and are capable of fermenting xylose, the second most abundant monosaccharide in woody plant material, which is of critical importance to the lignocellulosic biofuel industry [11,34,35]. Some traits are unique, such as a requirement for carbon dioxide in Cyniclomyces [36], while others, such as temperature preferences, evolve quickly [27–29].…”

Section: Metabolic Diversity Ecology and Biotechnologymentioning

confidence: 99%

Genomics and the making of yeast biodiversity

Hittinger

Rokas

Bai

et al. 2015

Current Opinion in Genetics & Development

102

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Yeasts are unicellular fungi that do not form fruiting bodies. Although the yeast lifestyle has evolved multiple times, most known species belong to the subphylum Saccharomycotina (syn. Hemiascomycota, hereafter yeasts). This diverse group includes the premier eukaryotic model system, Saccharomyces cerevisiae; the common human commensal and opportunistic pathogen, Candida albicans; and over 1,000 other known species (with more continuing to be discovered). Yeasts are found in every biome and continent and are more genetically diverse than angiosperms or chordates. Ease of culture, simple life cycles, and small genomes (~10–20 Mbp) have made yeasts exceptional models for molecular genetics, biotechnology, and evolutionary genomics. Here we discuss recent developments in understanding the genomic underpinnings of the making of yeast biodiversity, comparing and contrasting natural and human-associated evolutionary processes. Only a tiny fraction of yeast biodiversity and metabolic capabilities has been tapped by industry and science. Expanding the taxonomic breadth of deep genomic investigations will further illuminate how genome function evolves to encode their diverse metabolisms and ecologies.

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Section: Metabolic Diversity Ecology and Biotechnologymentioning

confidence: 99%

Genomics and the making of yeast biodiversity

Hittinger

Rokas

Bai

et al. 2015

Current Opinion in Genetics & Development

102

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“…Recently, genome sequencing of several yeasts has been reported, including for Spathaspora passalidarum (Wohlbach et al 2011), Scheffersomyces stipitis (Jeffries et al 2007), Candida tenuis (Wohlbach et al 2011) and M. guilliermondii (Butler et al 2009). The genus Spathaspora has been described recently (Nguyen et al 2006), and only a few species have been identified of which only a few genomes are available (Lobo et al 2014;Lopes et al 2017). Moreover, among these, only S. passalidarum presents a high quality genome annotation (Wohlbach et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Physiological and comparative genomic analysis of new isolated yeasts Spathaspora sp. JA1 and Meyerozyma caribbica JA9 reveal insights into xylitol production

Trichez

Steindorff

Soares

et al. 2019

FEMS Yeast Research

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Xylitol is a five-carbon polyol of economic interest that can be produced by microbial xylose reduction from renewable resources. The current study sought to investigate the potential of two yeast strains, isolated from Brazilian Cerrado biome, in the production of xylitol as well as the genomic characteristics that may impact this process. Xylose conversion capacity by the new isolates Spathaspora sp. JA1 and Meyerozyma caribbica JA9 was evaluated and compared with control strains on xylose and sugarcane biomass hydrolysate. Among the evaluated strains, Spathaspora sp. JA1 was the strongest xylitol producer, reaching product yield and productivity as high as 0.74 g/g and 0.20 g/(L.h) on xylose, and 0.58 g/g and 0.44 g/(L.h) on non-detoxified hydrolysate. Genome sequences of Spathaspora sp. JA1 and M. caribbica JA9 were obtained and annotated. Comparative genomic analysis revealed that the predicted xylose metabolic pathway is conserved among the xylitol-producing yeasts Spathaspora sp. JA1, M. caribbica JA9 and Meyerozyma guilliermondii, but not in Spathaspora passalidarum, an efficient ethanol-producing yeast. Xylitol-producing yeasts showed strictly NADPH-dependent xylose reductase and NAD+-dependent xylitol-dehydrogenase activities. This imbalance of cofactors favors the high xylitol yield shown by Spathaspora sp. JA1, which is similar to the most efficient xylitol producers described so far.

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“…passalidarum and Sp. arborariae genome sequences [21,37], we designed primers to amplify the SaXYL1 and SpXYL1.1 genes (both open reading frames -ORFswith 957 nucleotides, encoding for proteins of 318 amino acids), and the SpXYL2.2 gene (an ORF with 1089 nucleotides, encoding for a protein of 362 amino acids), and cloned these three genes into the pPGK multicopy plasmid for expression in the S. cerevisiae CEN.PK2-1C yeast strain. As shown in Table 3, these genes were functional in S. cerevisiae, with a xylose reductase activity encoded by the SaXYL1 gene that accepts both co-substrates, with a NADH-dependent activity that is~30% the activity measured with NADPH, while the xylose reductase encoded by the SpXYL1.1 gene accepted only NADPH as co-substrate.…”

Section: Resultsmentioning

confidence: 99%

“…Based on the genome sequence of Sp. arborariae [37] and Sp. passalidarum [21], primers were designed (Table 1) to amplify the xylose reductase encoded by the SaXYL1 or SpXYL1.1 genes, and the xylitol dehydrogenase encoded by the SpXYL2.2 gene, introducing restriction sites for cloning into multicopy shuttle vectors containing strong and constitutive promoters and terminators (pPGK, p423-TEF and p423-GPD, Table 1), as well as the URA3 or HIS3 genes used as selective markers.…”

Section: Molecular Biology Techniquesmentioning

confidence: 99%

Combining Xylose Reductase from Spathaspora arborariae with Xylitol Dehydrogenase from Spathaspora passalidarum to Promote Xylose Consumption and Fermentation into Xylitol by Saccharomyces cerevisiae

et al. 2020

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In recent years, many novel xylose-fermenting yeasts belonging to the new genus Spathaspora have been isolated from the gut of wood-feeding insects and/or wood-decaying substrates. We have cloned and expressed, in Saccharomyces cerevisiae, a Spathaspora arborariae xylose reductase gene (SaXYL1) that accepts both NADH and NADPH as co-substrates, as well as a Spathaspora passalidarum NADPH-dependent xylose reductase (SpXYL1.1 gene) and the SpXYL2.2 gene encoding for a NAD+-dependent xylitol dehydrogenase. These enzymes were co-expressed in a S. cerevisiae strain over-expressing the native XKS1 gene encoding xylulokinase, as well as being deleted in the alkaline phosphatase encoded by the PHO13 gene. The S. cerevisiae strains expressing the Spathaspora enzymes consumed xylose, and xylitol was the major fermentation product. Higher specific growth rates, xylose consumption and xylitol volumetric productivities were obtained by the co-expression of the SaXYL1 and SpXYL2.2 genes, when compared with the co-expression of the NADPH-dependent SpXYL1.1 xylose reductase. During glucose-xylose co-fermentation by the strain with co-expression of the SaXYL1 and SpXYL2.2 genes, both ethanol and xylitol were produced efficiently. Our results open up the possibility of using the advantageous Saccharomyces yeasts for xylitol production, a commodity with wide commercial applications in pharmaceuticals, nutraceuticals, food and beverage industries.

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Draft Genome Sequence of the d -Xylose-Fermenting Yeast Spathaspora arborariae UFMG-HM19.1A ^T

Cited by 14 publications

References 18 publications

Genomics and the making of yeast biodiversity

Genomics and the making of yeast biodiversity

Physiological and comparative genomic analysis of new isolated yeasts Spathaspora sp. JA1 and Meyerozyma caribbica JA9 reveal insights into xylitol production

Combining Xylose Reductase from Spathaspora arborariae with Xylitol Dehydrogenase from Spathaspora passalidarum to Promote Xylose Consumption and Fermentation into Xylitol by Saccharomyces cerevisiae

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Draft Genome Sequence of the d -Xylose-Fermenting Yeast Spathaspora arborariae UFMG-HM19.1A T

Cited by 14 publications

References 18 publications

Genomics and the making of yeast biodiversity

Genomics and the making of yeast biodiversity

Physiological and comparative genomic analysis of new isolated yeasts Spathaspora sp. JA1 and Meyerozyma caribbica JA9 reveal insights into xylitol production

Combining Xylose Reductase from Spathaspora arborariae with Xylitol Dehydrogenase from Spathaspora passalidarum to Promote Xylose Consumption and Fermentation into Xylitol by Saccharomyces cerevisiae

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Draft Genome Sequence of the d -Xylose-Fermenting Yeast Spathaspora arborariae UFMG-HM19.1A ^T