Draft Genome Sequence of the First NDM-1-Producing Providencia stuartii Strain Isolated in Portugal

Manageiro, Vera; Sampaio, Daniel A.; Pereira, Patrícia; Rodrigues, Paulo M. M.; Vieira, Luı́s; Palos, Carlos; Caniça, Manuela

doi:10.1128/genomea.01077-15

Cited by 23 publications

(26 citation statements)

References 7 publications

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“…Providencia species are frequently found in environmental settings, but are also opportunistic human pathogens, mainly as the causative agents of urinary tract infections ( Wie 2015 ). They are not among the most significant or prevalent human threats, but recently they have been attracting interest due to increasing reports of P. rettgeri NDM-positive isolates found around the world ( Barrios et al 2013 ; Carvalho-Assef et al 2013 ; Mataseje et al 2014 ; Pollett et al 2014 ; Tada et al 2014 ; Carmo Junior et al 2015 ; Manageiro et al 2015 ; Nachimuthu et al 2015 ; Wailan, Paterson, et al 2016). Here, we also found P. rettgeri to be the most frequent bacteria harboring the bla NDM-1 gene in three hospitals at distant locations from each other in Colombia.…”

Section: Discussionmentioning

confidence: 99%

“…NDM-positive Enterobacteriaceae , particularly Providencia spp. play an increasingly important role in multidrug resistant infections and dissemination of bla NDM around the world, as evidenced by the rapidly accumulating reports of isolation of this bacteria harboring this gene ( Carvalho-Assef et al 2013 ; Mataseje et al 2014 ; Pollett et al 2014 ; Tada et al 2014 ; Carmo Junior et al 2015 ; Manageiro et al 2015 ; Nachimuthu et al 2015 ; Wailan, Paterson, et al 2016). Previously, we reported the first South American bla NDM-1 outbreak, which occurred in Colombia in Klebsiella pneumonia , as well as an NDM-1-positive Providencia rettgeri outbreak in Mexico, both of which occurred in 2011–2012, without any link to the Indian subcontinent ( Barrios et al 2013 ; Escobar Perez et al 2013 ).…”

Section: Introductionmentioning

confidence: 99%

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Genomic Epidemiology of NDM-1-Encoding Plasmids in Latin American Clinical Isolates Reveals Insights into the Evolution of Multidrug Resistance

Márquez-Ortiz

Haggerty

Olarte³

et al. 2017

Genome Biology and Evolution

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Bacteria that produce the broad-spectrum Carbapenem antibiotic New Delhi Metallo-β-lactamase (NDM) place a burden on health care systems worldwide, due to the limited treatment options for infections caused by them and the rapid global spread of this antibiotic resistance mechanism. Although it is believed that the associated resistance gene blaNDM-1 originated in Acinetobacter spp., the role of Enterobacteriaceae in its dissemination remains unclear. In this study, we used whole genome sequencing to investigate the dissemination dynamics of blaNDM-1-positive plasmids in a set of 21 clinical NDM-1-positive isolates from Colombia and Mexico (Providencia rettgeri, Klebsiella pneumoniae, and Acinetobacter baumannii) as well as six representative NDM-1-positive Escherichia coli transconjugants. Additionally, the plasmids from three representative P. rettgeri isolates were sequenced by PacBio sequencing and finished. Our results demonstrate the presence of previously reported plasmids from K. pneumoniae and A. baumannii in different genetic backgrounds and geographically distant locations in Colombia. Three new previously unclassified plasmids were also identified in P. rettgeri from Colombia and Mexico, plus an interesting genetic link between NDM-1-positive P. rettgeri from distant geographic locations (Canada, Mexico, Colombia, and Israel) without any reported epidemiological links was discovered. Finally, we detected a relationship between plasmids present in P. rettgeri and plasmids from A. baumannii and K. pneumoniae. Overall, our findings suggest a Russian doll model for the dissemination of blaNDM-1 in Latin America, with P. rettgeri playing a central role in this process, and reveal new insights into the evolution and dissemination of plasmids carrying such antibiotic resistance genes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genomic Epidemiology of NDM-1-Encoding Plasmids in Latin American Clinical Isolates Reveals Insights into the Evolution of Multidrug Resistance

Márquez-Ortiz

Haggerty

Olarte³

et al. 2017

Genome Biology and Evolution

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“…Their association with β-lactamases, such as the bla NDM , has described in K. pneumoniae, E. coli, and Providencia stuartii isolates from countries as diverse as India, Canada, Kenya, New Zealand, Australia, and the United States. [35][36][37] Although the contig bearing the A/C 2 replicase gene did not harbor the class 1 integrase and associated gene cassettes, we would not be surprised if further genomic sequencing proves this to be the case.…”

Section: Genomic Locationmentioning

confidence: 94%

Genomic analysis of a multidrug‐resistant clinical Providencia rettgeri (PR002) strain with the novel integron ln1483 and an A/C plasmid replicon

Mbelle

Sekyere

Amoako

et al. 2019

Annals of the New York Academy of Sciences

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Whole‐genome sequence analysis was performed on a multidrug‐resistant Providencia rettgeri PR002 clinical strain isolated from the urine of a hospitalized patient in Pretoria, South Africa, in 2013. The resistome, mobilome, pathogenicity island(s), as well as virulence and heavy‐metal resistance genes of the isolate, were characterized using whole‐genome sequencing and bioinformatic analysis. PR002 had a genome assembly size of 4,832,624 bp with a GC content of 40.7%, an A/C2 plasmid replicase gene, four integrons/gene cassettes, 17 resistance genes, and several virulence and heavy metal resistance genes, confirming PR002 as a human pathogen. A novel integron, In1483, harboring the gene blaOXA‐2, was identified, with other uncharacterized class 1 integrons harboring aacA4cr and dfrA1. Aac(3′)‐IIa and blaSCO‐1, as well as blaPER‐7, sul2, and tet(B), were found bracketed by composite Tn3 transposons, and IS91, IS91, and IS4 family insertion sequences, respectively. PR002 was resistant to all antibiotics tested except amikacin, carbapenems, cefotaxime‐clavulanate, ceftazidime‐clavulanate, cefoxitin, and fosfomycin. PR002 was closely related to PR1 (USA), PRET_2032 (SPAIN), DSM_1131, and NCTC7477 clinical P. rettgeri strains, but not close enough to suggest it was imported into South Africa from other countries. Multidrug resistance in P. rettgeri is rare, particularly in clinical settings, making this case an important incident requiring urgent attention. This is also the first report of an A/C plasmid in P. rettgeri. The array, multiplicity, and diversity of resistance and virulence genes in this strain are concerning, necessitating stringent infection control, antibiotic stewardship, and periodic resistance surveillance/monitoring policies to preempt further horizontal and vertical spread of these resistance genes.

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“…This MDR phenotype can be further exaggerated in clinical isolates, a majority of which were shown to feature plasmid-encoded extended-spectrum β-lactamases (ESBLs) [17]. More recently, clinical isolates presenting carbapenemase activities were isolated in Afghanistan [18] and Portugal [19]. P .…”

Section: Introductionmentioning

confidence: 99%

Providencia stuartii form biofilms and floating communities of cells that display high resistance to environmental insults

Khatib¹,

Tran²,

Nasrallah³

et al. 2017

PLoS ONE

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Biofilms are organized communities of bacterial cells that are responsible for the majority of human chronic bacterial infections. Providencia stuartii is a Gram-negative biofilm-forming bacterium involved in high incidence of urinary tract infections in catheterized patients. Yet, the structuration of these biofilms, and their resistance to environmental insults remain poorly understood. Here, we report on planktonic cell growth and biofilm formation by P. stuartii, in conditions that mimic its most common pathophysiological habitat in humans, i.e. the urinary tract. We observed that, in the planktonic state, P. stuartii forms floating communities of cells, prior to attachment to a surface and subsequent adoption of the biofilm phenotype. P. stuartii planktonic and biofilm cells are remarkably resistant to calcium, magnesium and to high concentrations of urea, and show the ability to grow over a wide range of pHs. Experiments conducted on a P. stuartii strain knocked-out for the Omp-Pst2 porin sheds light on the role it plays in the early stages of growth, as well as in the adaptation to high concentration of urea and to varying pH.

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Draft Genome Sequence of the First NDM-1-Producing Providencia stuartii Strain Isolated in Portugal

Cited by 23 publications

References 7 publications

Genomic Epidemiology of NDM-1-Encoding Plasmids in Latin American Clinical Isolates Reveals Insights into the Evolution of Multidrug Resistance

Genomic Epidemiology of NDM-1-Encoding Plasmids in Latin American Clinical Isolates Reveals Insights into the Evolution of Multidrug Resistance

Genomic analysis of a multidrug‐resistant clinical Providencia rettgeri (PR002) strain with the novel integron ln1483 and an A/C plasmid replicon

Providencia stuartii form biofilms and floating communities of cells that display high resistance to environmental insults

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