Draft Genome Sequence of Pseudoalteromonas luteoviolacea HI1, Determined Using Roche 454 and PacBio Single-Molecule Real-Time Hybrid Sequencing

Asahina, Audrey; Hadfield, Michael G.

doi:10.1128/genomea.01590-14

Cited by 8 publications

(6 citation statements)

References 16 publications

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“…Nineteen genomes of P . luteoviolacea strains have been isolated and sequenced from oceans around the world (Rypien et al ., 2010; Tran and Hadfield, 2011; Asahina and Hadfield, 2015; Maansson et al ., 2016; Thøgersen et al ., 2016) and display a significant diversity in gene content (Maansson et al ., 2016; Busch et al ., 2019). The chemical activity, stimulatory nature, genetic tractability and genomic diversity of P .…”

Section: Introductionmentioning

confidence: 99%

Genetic examination of the marine bacterium Pseudoalteromonas luteoviolacea and effects of its metamorphosis‐inducing factors

Alker

Delherbe

Purdy

et al. 2020

Environmental Microbiology

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Summary Pseudoalteromonas luteoviolacea is a globally distributed marine bacterium that stimulates the metamorphosis of marine animal larvae, an important bacteria–animal interaction that can promote the recruitment of animals to benthic ecosystems. Recently, different P. luteoviolacea isolates have been shown to produce two stimulatory factors that can induce tubeworm and coral metamorphosis; Metamorphosis‐Associated Contractile structures (MACs) and tetrabromopyrrole (TBP) respectively. However, it remains unclear what proportion of P. luteoviolacea isolates possess the genes encoding MACs, and what phenotypic effect MACs and TBP have on other larval species. Here, we show that 9 of 19 sequenced P. luteoviolacea genomes genetically encode both MACs and TBP. While P. luteoviolacea biofilms producing MACs stimulate the metamorphosis of the tubeworm Hydroides elegans, TBP biosynthesis genes had no effect under the conditions tested. Although MACs are lethal to larvae of the cnidarian Hydractinia symbiologicarpus, P. luteoviolacea mutants unable to produce MACs are capable of stimulating metamorphosis. Our findings reveal a hidden complexity of interactions between a single bacterial species, the factors it produces and two species of larvae belonging to different phyla.

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Section: Introductionmentioning

confidence: 99%

Genetic examination of the marine bacterium Pseudoalteromonas luteoviolacea and effects of its metamorphosis‐inducing factors

Alker

Delherbe

Purdy

et al. 2020

Environmental Microbiology

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“…Preparations of C. lytica were also examined with scanning electron microscopy (SEM) to verify the origin of the vesicles. In addition, the full genomes of the Hawaiian strains of both P. luteoviolacea 43 and C. lytica were available 44 , as well as, the genomes for the types strains of both Gram-positive bacteria, allowing a search for the tailocin gene assembly. This study further addresses the question, if tailocins are not transcribed in other inductive bacterial species, what are the mechanisms by which they induce larvae of the tubeworm Hydroides elegans to attach to surfaces and metamorphose?…”

mentioning

confidence: 99%

Induction of Invertebrate Larval Settlement; Different Bacteria, Different Mechanisms?

Freckelton

Nedved

Hadfield

2017

Sci Rep

Self Cite

101

102

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Recruitment via settlement of pelagic larvae is critical for the persistence of benthic marine populations. For many benthic invertebrates, larval settlement occurs in response to surface microbial films. Larvae of the serpulid polychaete Hydroides elegans can be induced to settle by single bacterial species. Until now, only Pseudoalteromonas luteoviolacea had been subjected to detailed genetic and mechanistic studies. To determine if the complex structures, termed tailocins, derived from phage-tail gene assemblies and hypothesized to be the settlement cue in P. luteoviolacea were present in all inductive bacteria, genomic comparisons with inductive strains of Cellulophaga lytica, Bacillus aquimaris and Staphylococcus warneri were undertaken. They revealed that the gene assemblies for tailocins are lacking in these other bacteria. Negatively stained TEM images confirmed the absence of tailocins and revealed instead large numbers of extracellular vesicles in settlement-inductive fractions from all three bacteria. TEM imaging confirmed for C. lytica that the vesicles are budded from cell surfaces in a manner consistent with the production of outer membrane vesicles. Finding multiple bacteria settlement cues highlights the importance of further studies into the role of bacterial extracellular vesicles in eliciting settlement and metamorphosis of benthic marine larvae.

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“…The bacterium Pseudoalteromonas luteoviolacea strain HI1, a highly inductive bio lm species, isolated and genetically characterized in the Had eld lab 13,33,34 presents a challenge to interpret. However, examination of the bacterial cues themselves provides important insight and criteria for assessing the potential for inductive cues to explain bacterial induction of metamorphosis on a broad scale.…”

Section: Discussionmentioning

confidence: 99%

Bacterial polysaccharide cues invertebrate settlement and metamorphosis

Hadfield,

Freckelton,

Nedved

2023

Preprint

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Metamorphosis for many marine invertebrates depends on exposure to an external cue, commonly produced by bacteria. For larvae of the marine tubeworm Hydroides elegans, lipopolysaccharide (LPS) from the biofilm-dwelling, Gram-negative bacterium Cellulophaga lytica was previously demonstrated to induce metamorphosis. To determine whether LPS is a common metamorphosis-inducing factor in this species, we compared LPS from inductive and non-inductive Gram-negative marine biofilm bacteria (Cellulophaga lytica, Thalassotalea euphilliae, Pseudoalteromonas luteoviolacea, and Tenacibaculum aiptasiae) with commercial LPS from human pathogens (Escherichia coli, Salmonella enterica, and Pseudomonas aeruginosa). LPS from inductive bacteria triggered metamorphosis, while LPS from non-inductive bacteria did not. Furthermore, we show that the inductive property of LPS resides within the polysaccharide (O-antigen) component from multiple species, suggesting a crucial role for conserved polysaccharide elements in triggering metamorphosis in H. elegans. These results provide insights into bacterial involvement in animal development and how marine benthic communities are established and maintained.

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Draft Genome Sequence of Pseudoalteromonas luteoviolacea HI1, Determined Using Roche 454 and PacBio Single-Molecule Real-Time Hybrid Sequencing

Cited by 8 publications

References 16 publications

Genetic examination of the marine bacterium Pseudoalteromonas luteoviolacea and effects of its metamorphosis‐inducing factors

Genetic examination of the marine bacterium Pseudoalteromonas luteoviolacea and effects of its metamorphosis‐inducing factors

Induction of Invertebrate Larval Settlement; Different Bacteria, Different Mechanisms?

Bacterial polysaccharide cues invertebrate settlement and metamorphosis

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